LOCUS       sequence-465262-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ZDHHC13 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Zhang Z, Ren C, Xiao R, Ma S, Liu H, Dou Y, Fan Y, Wang S, Zhan P, 
            Gao C, Yue X, Li C, Gao L, Liang X, Wu Z, Ma C
  TITLE     Palmitoylation of TIM-3 promotes immune exhaustion and restrains 
            antitumor immunity.
  JOURNAL   Sci Immunol. 2024 Nov 15;9(101):eadp7302. doi: 
            10.1126/sciimmunol.adp7302. Epub 2024 Nov 15.
  PUBMED    39546589
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2024-11-15"; volume: "9(101):eadp7302. doi"; 
            pages: " 10.1126/sciimmunol.adp7302. Epub 2024 Nov 15"
COMMENT     Sequence Label: Flag-ZDHHC13
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..169
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     161..178
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     162..178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        339..718
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        719..922
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     873..893
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             952..1017
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2943..3565
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3142..3161)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3589..3609)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3594..3923
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3774..3909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3961..3980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3962..3980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3994..4010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3994..4010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4007..4029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4018..4034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4042..4048,4049..4066,4067..4072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4087..4108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4225..4242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4396..4984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4476..4495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5155..5946,5947..6015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5778..5797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6016..6120)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6147..6575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6234..6253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6444..6465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
       61 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
      121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
      181 caagctgatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat
      241 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta
      301 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta
      361 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      421 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      481 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      541 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
      601 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      661 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      721 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
      781 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      841 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
      901 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag aattaaccat ggactacaaa
      961 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt
     1021 gcggccgcga attcaatgga ggggccgggg ctgggctcgc agtgcaggaa tcacagccat
     1081 ggcccccacc ctccaggatt tggtcgatat ggcatctgtg cacatgaaaa caaagaactt
     1141 gccaatgcaa gagaagctct tcctcttata gaggactcta gtaactgtga cattgtcaaa
     1201 gctactcaat acggaatttt tgaacgatgt aaagagttgg tagaagcagg atatgatgtc
     1261 aggcaaccag ataaagaaaa tgtgtcgctt cttcattggg ctgctattaa caacagactg
     1321 gatcttgtaa agttttatat ttcaaaaggt gctgttgtag atcagttggg tggagattta
     1381 aattcaactc ctcttcactg ggccatccga caaggacatt tacctatggt catattatta
     1441 ctccagcatg gtgcagaccc cactcttatt gatggagagg gattcagcag catccacctg
     1501 gcagtattgt ttcaacacat gcctattata gcatatctca tctcaaaggg acagagtgtg
     1561 aatatgacag atgtaaatgg gcagacacct ctcatgttat cagctcacaa agtaattggg
     1621 ccagaaccaa ctggatttct tttaaagttt aatccttctc tcaatgtggt tgataaaata
     1681 caccaaaaca ctccacttca ctgggcagtt gcagcaggaa atgttaatgc agttgataag
     1741 cttttggaag ctggttctag cctggatatc cagaatgtta agggagaaac acctcttgat
     1801 atggctctac aaaacaaaaa tcagctcatt attcatatgc taaaaacaga agccaaaatg
     1861 agagccaacc aaaagttcag actttggagg tggctgcaga aatgcgagct cttcctgctg
     1921 ctgatgcttt ctgtgattac catgtgggct attggataca tattggactt caattcagat
     1981 tcttggcttt taaaaggatg tcttctagta acactgtttt ttctgacatc tttgtttcca
     2041 aggttcttgg ttgggtataa gaaccttgta tacttaccaa cagcctttct gctaagttct
     2101 gttttttgga tatttatgac ttggttcatc ttattttttc ctgatttagc aggagcccct
     2161 ttctatttca gtttcatttt cagcatagta gcctttctat actttttcta taagacttgg
     2221 gcaactgatc caggcttcac taaggcttct gaagaagaaa agaaagtgaa tatcatcacc
     2281 cttgcagaaa ctggctctct ggacttcagg acattttgta catcatgtct tataaggaag
     2341 ccattaaggt cactccactg ccatgtatgc aactgctgtg tggctcgata tgatcaacac
     2401 tgcctgtgga ctggacggtg cataggtttt ggcaaccatc actattacat attcttcttg
     2461 tttttccttt ccatggtatg tggctggatt atatatggat ctttcatcta tttgtccagt
     2521 cattgtgcca caacattcaa agaagatgga ttatggactt acctcaatca gattgtggcc
     2581 tgttcccctt gggttttata tatcttgatg ctagcaactt tccatttctc atggtcaaca
     2641 tttttattat taaatcaact ctttcagatt gcctttctgg gcctgacctc ccatgagaga
     2701 atcagcctgc agaagcagag caagcatatg aaacagacgt tgtccctcag gaagacacca
     2761 tacaatcttg gattcatgca gaacctggca gatttctttc agtgtggctg ctttggcttg
     2821 gtgaagccct gtgtggtaga ttggacatca cagtatacca tggtctttca cccagccagg
     2881 gagaaggttc ttcgctcagt atgaagatct gatatcggta ccagtcgact ctagaggatc
     2941 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     3001 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     3061 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     3121 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     3181 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     3241 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     3301 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     3361 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     3421 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
     3481 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
     3541 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc
     3601 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3661 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3721 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3841 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3901 ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt
     3961 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     4021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6121 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     6181 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6241 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6301 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6361 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6421 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6481 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6541 aaatgagctg atttaacaaa aatttaacgc gaattttaac a
//
