LOCUS       sequence-465270-        6531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human atlastin GTPase 2 (ATL2) 
            fused to myc-tag under the spleen focus-forming virus (SFFV) 
            promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-ATL2-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..6531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..128
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        418..436
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     418..435
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             461..694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(789..1502,1503..1505,1506..1508))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(834..855)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1183..1202
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1442..1463
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1505..1514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1688..1707)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     polyA_signal    1954..2088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1978..1997)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2032..2051
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2513..2533)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2663..2728
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(3862..4191)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3876..4011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4176..4196
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(4266..4283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4437..5025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4517..4536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5196..5987,5988..6056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5819..5838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6057..6161)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6229..6247
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6285..6307)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6407..6426
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
       61 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
      121 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
      181 gtagaggatc tggctagcga tgaccctgct gattggttcg ctgaccattt ccgggtgcgg
      241 gacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta
      301 cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat
      361 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      421 taggtgacac tatagaatac acggaattaa ttctagatgc tgctagagat tttccacact
      481 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
      541 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
      601 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
      661 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
      721 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct cgaattaatt
      781 ccctcgagtt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      841 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      901 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      961 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcgggtctt gaagttcacc
     1021 ttgatgccgt tcttctgctt gtcggcggtg atatagacct tgtggctgtt gtagttgtac
     1081 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     1141 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     1201 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     1261 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcacgccgta ggtcaaggtg
     1321 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     1381 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     1441 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     1501 ctcaccatgg tggcgaattg atccgagctc ggtaccaagc ttcgtctaac aaaaaagcca
     1561 aaaacggcca gaatttagcg gacaatttac tagtctaaca ctgaaaatta catattgacc
     1621 caaatgatta catttcaaaa ggtgcctaaa aaacttcaca aaacacactc gccaaccccg
     1681 agcgcatagt tcaaaaccgg agcttcagct acttaagaag ataggtacat aaaaccgacc
     1741 aaagaaactg acgcctcact tatccctccc ctcaccagag gtccggcgcc tgtcgattca
     1801 ggagagccta ccctaggccc gaaccctgcg tcctgcgacg gagaaaagcc taccgcacac
     1861 ctaccggcag gtggcccaca ggtaattggc agtggtaata gggtatgggg tgggaagttt
     1921 gggatgattt tggttagctt gagttatcca gttgatccag acatgataag atacattgat
     1981 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2101 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2161 aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
     2221 attccttatt aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt
     2281 tattaatagc agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat
     2341 aactgttatg cctacttata aaggttacag aatatttttc cataattttc ttgtatagca
     2401 gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca
     2461 cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc
     2521 tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg
     2581 gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt
     2641 catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac
     2701 attatacact taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt
     2761 ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatccggggc ccactcataa
     2821 atccagttgc cgccacggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg
     2881 gcatcgctca tcacaatacg tgcctggacg tcgaggattt cgcgtgggtc aatgccgcgc
     2941 cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg
     3001 gccgactgca caggcggttg tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca
     3061 ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca
     3121 gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc
     3181 cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc
     3241 catatcccac ggctgttcaa tccaggtatc ttgcgggata tcaacaacat agtcatcaac
     3301 cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc
     3361 acgaatcgca accgcagtac caccggtatc caccaggtca tcaataacga tgaagccttc
     3421 gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct
     3481 ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg
     3541 tacagaccgc cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg
     3601 cttgcgagtt tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc
     3661 atgtgaagtg tcccagcctg tttatctacg gcttaaaaag tgttcgaggg gaaaataggt
     3721 tgcgcgagat tatagagatc tggcgcacta aaaaccagta tttcacatga gtccgcgtct
     3781 ttttacgcac tgcctctccc tgacgcggga taaagtggta ttctcaaaca tatctcgcaa
     3841 gcctgtcttg tgtccaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     3901 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     3961 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4021 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4081 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     4141 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     4201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6181 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6241 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6301 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6361 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6421 attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca ttaggaagca
     6481 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg c
//
