LOCUS       sequence-465304-        5618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an mCherry-tagged Prohibitin2/PHB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne
            R, Tramier M
  TITLE     Mitochondrial Aurora kinase A induces mitophagy by interacting with 
            MAP1LC3 and Prohibitin 2.
  JOURNAL   Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 
            10.26508/lsa.202000806. Print 2021 Jun.
  PUBMED    33820826
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-04-5"; volume: "4(6):e202000806. doi"; 
            pages: " 10.26508/lsa.202000806. Print 2021 Jun"
COMMENT     Sequence Label: PHB2-mCherry
FEATURES             Location/Qualifiers
     source          1..5618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(621..640)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    665..712
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1041..1629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1530..1549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1804..2107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2108..2311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2262..2282
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3310..3319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3316..4026
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3461..3479)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3729..3748
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4149..4270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4186..4205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4240..4259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4277..4732)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4414..4435)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4626..4645
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4759..4863
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4865..5222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4865..5056
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4888..4908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5073..5208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5257..5618,1..433)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(5257..5618,1..433)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5311..5330)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
       61 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      121 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      181 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      241 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      301 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      361 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      421 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
      481 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      541 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      601 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
      661 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
      721 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
      781 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      841 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
      901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     1741 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1801 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1861 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1921 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1981 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2041 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2101 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2161 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2221 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2281 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     2341 ctaccggact cagatctcga gatggcccag aacttgaagg acttggcggg acggctgccc
     2401 gccgggcccc ggggcatggg cacggccctg aagctgttgc tgggggccgg cgccgtggcc
     2461 tacggtgtgc gcgaatctgt gttcaccgtg gaaggcgggc acagagccat cttcttcaat
     2521 cggatcggtg gagtgcagca ggacactatc ctggccgagg gccttcactt caggatccct
     2581 tggttccagt accccattat ctatgacatt cgggccagac ctcgaaaaat ctcctcccct
     2641 acaggctcca aagacctaca gatggtgaat atctccctgc gagtgttgtc tcgacccaat
     2701 gctcaggagc ttcctagcat gtaccagcgc ctagggctgg actacgagga acgagtgttg
     2761 ccgtccattg tcaacgaggt gctcaagagt gtggtggcca agttcaatgc ctcacagctg
     2821 atcacccagc gggcccaggt atccctgttg atccgccggg agctgacaga gagggccaag
     2881 gacttcagcc tcatcctgga tgatgtggcc atcacagagc tgagctttag ccgagagtac
     2941 acagctgctg tagaagccaa acaagtggcc cagcaggagg cccagcgggc ccaattcttg
     3001 gtagaaaaag caaagcagga acagcggcag aaaattgtgc aggccgaggg tgaggccgag
     3061 gctgccaaga tgcttggaga agcactgagc aagaaccctg gctacatcaa acttcgcaag
     3121 attcgagcag cccagaatat ctccaagacg atcgccacat cacagaatcg tatctatctc
     3181 acagctgaca accttgtgct gaacctacag gatgaaagtt tcaccagggg aagtgacagc
     3241 ctcatcaagg gtaagaaaaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     3301 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     3361 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3421 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3481 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     3541 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3601 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3661 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     3721 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     3781 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     3841 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     3901 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     3961 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     4021 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     4081 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4141 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4261 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4321 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4381 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4441 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4501 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4561 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4621 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4681 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     4741 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4801 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4861 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4981 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5041 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5101 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5161 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5221 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5281 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5401 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     5461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5581 cctgccgaga aagtatccat catggctgat gcaatgcg
//
