LOCUS       sequence-465435-       10087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10087)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG/MBP-VPS15(R103K)-TSF
FEATURES             Location/Qualifiers
     source          1..10087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4110..4130)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4194..5294)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MGIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     complement(4197..4220)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      5291..5300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5348..5367)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5375..6391
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5429..5451)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        6662..7041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7072..7176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7177..7245,7246..8037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7395..7414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8208..8796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8697..8716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8950..8967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9035..9169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9059..9078)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9113..9132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(9175..9370)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9189..9324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    9429..9450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9465..9470,9471..9488,9489..9495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9503..9519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9508..9530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9527..9543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9527..9543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9886..9905
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    9904..9959
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     10005..10024
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 tgcacctgag gagtgaattc ctcgagtcac ttccacactt tcacgatgcc gtctctggag
       61 gcggtcacga tgaagccctg ggtagtctgg aatgtagcca catcggtaat gatgtcatgg
      121 tgtcccactg gcagtgactc aggtcccctc cttggagtat cgtcacttgg tcccactttc
      181 tgcttgttct ggatttcctg caccacttct gtgccctcaa tgatcttccg atagtagctc
      241 actgaggggg agctggttga gccggccacc acataactgc gctctgggta agccagatcc
      301 caaaacctaa ttttcatgtc agagccggcg gtcagcagga ttggatttcc atcagcaggg
      361 gaacagtaga tcccgtgcac tgaatgtgga ctaggctgca gctcagacag gggtggggct
      421 gaactagccc acagtgtgaa tctccgatct ccggtctcca tgtcccacat ggacacttca
      481 ttgttcccct gcacagcggc gatgacccaa ctctgataca gagggtgcat agacagcctc
      541 ctaatcctag ccctgcttgg gtgacaatga gaggagattg gcagctgaaa gcgcatgtcc
      601 cagcaagcca tagtcccgct tgatgtgccg atacacagcc agcactggtg aatatccacg
      661 gcgaaagagg tgatcaggcc ggatttcagg tcatgcttca gagtccaagc gttactagag
      721 gagcgcagat cccatcccac cagagaccca ttcactgtag cgtaggccag cactgactgg
      781 gccccactgt tgaaatggtg catgtccacc acgcagccat cctccttctg gtccagaatc
      841 ctggactgca gggggtggat cttagggctt ttgggcagct ttgaggcttc aattcccagc
      901 agctgcacag ccccgttgtc ggaagcgatg gccaggtaat ggctgccctg acaaaatgtc
      961 agggtcttca ctctgcctcc aatccgacta taggtcagga tagaccttgt ggtagttgtt
     1021 ttgccctcca tcttctgaga attccaaatc ttcacagttc catcgttgga gcatgtggcg
     1081 aacagagagt gctcgtcgga cactctgatc cgattcacgg cgcttttgtg ttcatgcagg
     1141 tgagccacca gcagtccctt tggcctccat ccaggtggtg gaggtttact ttcccactcg
     1201 gcattctcca tcatctgctt ggcaattctt tcagcgttac actgctcccg cttctgctgg
     1261 atcagctgct gcagttcggt tttgcaggta gtaatcctga tctggtatgt gcttggcaga
     1321 atggtgcttg acagcactgg gatcacaggt ttcttgttct gcactgtggt cacctcaggc
     1381 acctgactag atgtgctcag gggcacacaa atgccggcgg agctctctga tcttggtggc
     1441 ttcccggtct ggatcacttc ctgatcgctg ccttttggca gagcctgagg catattaggg
     1501 gggtccaggc ttccaaacat tgacttccat tcctcattca cgttgctgtc ctgtttcacg
     1561 tgcttgcggg cccttttatc gtcgggctcc tgtttagtct tcaccagatc cacctgcctc
     1621 cctgtaatgc ccagagcggc caggtcgatc acgcccttct gtgaactatc atgcaggtga
     1681 ctctggtcca cgatattagc tttggccttg ttagacttca tcatgaaatc tttcagggcc
     1741 agcagcttgt cttcctcttc ctcggtcatc ccctgagaca gcagtttctt cagcagctga
     1801 gcgatggctg gatcctctgg aggtgggcag tcaggcaggg atccgttgcg tttcttctgc
     1861 ctcatatgca ggtgtctaaa cagggaagtg atatccttgc tgcgcagggc gtagtcgaaa
     1921 atactcctag acacaggctc cttcagcacg gacagcagca ccagtttccg ttcaatctga
     1981 atgatgggct gtgtgatata gggatccagg tatggcatca gtttacagta cacgtcggcg
     2041 gtgctaatct gtctagccac cacagtgatg aatcccacgg ccccataccg aatccacaga
     2101 ttggggtggc acagaaatgg ggcgatatcg ctagcgaact cgtacacatg tggcttctgc
     2161 agcagtccca gctgacacat gcaggtcagg gcatacagag ctttcacgat cacaaattcc
     2221 tcggcgtcag acagcccctg ctgcagcaga ggcttcagaa tagaggagct ctgccagccc
     2281 acataagcgg ccactcccac gatgctatca aagaaggcgc cccgcaggtg ccaatcattc
     2341 ttgtcgttca ggaatgtgat catatgtgac agcagcacgt cgttagcttt ctggcgtcca
     2401 aagaacacac acagcctggt gatcccattc tccatcagag tctgcttcac aatgttttct
     2461 ggatcggaca gcagggtcac cactttctgc tgcaccattt cgtgcagggc ctgcagctct
     2521 gtgtcgtaat tcccgttagg atgggtcacc tcatcgattt cctcattgtt ggggtcgttt
     2581 tccatattca ggttcttcag ctgcaccagc tccagaaagc gcagggcggt ttcagccagc
     2641 agggcgatat tctcagcata ggccagcctc acaatagtgg catcgtcctg agccaggtgg
     2701 gcaatgccag gcaggatata ctcggggtag atattgatgt cgttgcgagg cacttccttc
     2761 accagggcca gcactttagt cagtgtcctc agagcctcgg ctctcacccg tggcacagaa
     2821 tcgttggaga agtgcagcag gtaaggggta attctgtcca gcaggatctc cacggacagc
     2881 cttggagcca gatgcaggat cagttccaga gcggccagtt tgctatcaca atacttcaga
     2941 gtctgcaggc aacttgtaat cacagacacc aggatgacca gcccattctc cttaggttcg
     3001 ccctcggctt tttcgggcag gtcgtggccg cacagattat gaatgatgtt tcccagatcc
     3061 tttctgatga ccaggatccg ttcgtcggcg ctcagaaaag tctctttagc gaactgggcc
     3121 atatagggct gcagaaatgt gtagaagatc tctgggaagg cgttgcctct ctgctgcttc
     3181 aggtaatctt cagcctccag gcgtttgtct ggttccctgt ggatcatctg tgtcaccagt
     3241 tcgcgaatgc tatgatcctc gatcttattc agcacctgct ctggaaagaa gtgtccgttc
     3301 ctataggcca gcagctgact cagatcaaac agaggcactc cttcggtgaa cagctcggca
     3361 atcacacacc cagcagaaaa gatgtccata gccctcttca gctctcctct cctcctctga
     3421 ttgctgttca gatccaccag gggtgttgat gggtctctca tgtattccag ctcggtggca
     3481 aacatcccgc catccacgaa ccgctcagga gcgatgtagc aggtccttct ccggctagtg
     3541 tcaaagaaat aattgaaatc ggcagggttg tcttcgggca ggtaagttgg cttaaaggaa
     3601 gcgaagtctg tcagcagcac ccaattccag ctggtcacca tcacgttctc agtcttgata
     3661 tcgccgtgtc tcactccaga tttatgggcc tggtccacag cagtcagaat ctgaaaggcg
     3721 atccaccgct tctcgatatt gttcaggaag ggtcttgtgg aaatccgatc atacaggttg
     3781 tcgcgcacgt actgcttaaa cagcatagcg gctttttcgg aggccttctc gctagctttc
     3841 tggaatggca ggcaattctg ggctgagttc agtctaatct tcagttcctc cagctcctgt
     3901 ttataactgg tcagaggcag agtgggatcc tggatagcga acaccttcac caccaccagg
     3961 ccttctctgt gtttagcccg ggccacctta aagaaccggg tgcttcccag tgatttgtca
     4021 tactcaaaat catggatgtc actgaagtaa gattccacgg acagaatctg gctaggagcg
     4081 atgccggcca gctgatttcc catggtacca ccctgaaaat acaggttttc ggtcgttggg
     4141 atatccccga ggttgttgtt attgttattg ttgttgttgt tcgagctcga attagtctgc
     4201 gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta
     4261 cgcacggcat accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg
     4321 gcgttttcca tagtggcggc aatacgtgga tctttcgcca actcttcctc gtaagacttc
     4381 agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc
     4441 agatagtttt cgaggaactc ttttgccagc tctttgttcg gactggcggc gttaatacct
     4501 gcgctcagca cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg cagtaccgtt
     4561 acaccataat tcactttgct ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc
     4621 atcgctgttt cgcctttatt aaaggcagct tctgcgatgg agtaatcggt gtctgcattc
     4681 atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta
     4741 tccacgccca cgtctttaat gtcgtacttg ccgttttcat acttgaacgc ataacccccg
     4801 tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc
     4861 ttacctttcg ctttcagttc tttatccagc gccgggatct cttcccaggt ttttggcggg
     4921 ttcggcagca gatctttgtt ataaatcagc gataacgctt caacagcgat cgggtaagca
     4981 atcagcttgc cgttgtaacg tacggcatcc caggtaaacg gatacagctt gtcctggaac
     5041 gctttgtccg gggtgatttc agccaacagg ccagattgag cgtagccacc aaagcggtcg
     5101 tgtgcccaga agataatgtc agggccatcg ccagttgccg caacctgtgg gaatttctct
     5161 tccagtttat ccggatgctc aacggtgact ttaattccgg tatctttctc gaatttctta
     5221 ccgacttcag cgagaccgtt atagccttta tcgccgttaa tccagattac cagtttacct
     5281 tcttcgatcc ccatggtggc tatcgatacc gtcgatcgag gaattctttg ccaaaatgat
     5341 gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa
     5401 catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc
     5461 cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg agagggagaa
     5521 ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc catttccttc ctgccggcgc
     5581 cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca gatttcggct
     5641 ccgcacagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc
     5701 aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct
     5761 cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc
     5821 accccccgcc cggcacggcg agccccgcgc cacgccccgt acggagcccc gcacccgaag
     5881 ccgggccgtg ctcagcaact cggggagggg ggtgcagggg gggttgcagc ccgaccgacg
     5941 cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct
     6001 cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg cgcacactgc
     6061 ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc
     6121 cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg ctccccccgc
     6181 acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc
     6241 gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct
     6301 gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg
     6361 gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg
     6421 gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg
     6481 ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc cccccccccc
     6541 gccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat aaatacaaaa
     6601 ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg ctcacctcga
     6661 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     6721 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     6781 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     6841 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     6901 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     6961 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     7021 aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     7081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9001 ctccccgcgc gttggccgat tcattaatgc agcggatcca gacatgataa gatacattga
     9061 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     9121 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta gctttttgca
     9181 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     9241 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     9301 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     9361 attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9421 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9481 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9541 gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga gaagagggac
     9601 agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga
     9661 aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatataaaaa aaatctaacc
     9721 tcaagtcaag gcttttctat ggaataagga atggacagca gggggctgtt tcatatactg
     9781 atgacctctt tatagccaac ctttgttcat ggcagccagc atatgggcat atgttgccaa
     9841 actctaaacc aaatactcat tctgatgttt taaatgattt gccctcccat atgtccttcc
     9901 gagtgagaga cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta
     9961 gccagaagtc agatgctcaa ggggcttcat gatgtcccca taatttttgg cagagggaaa
    10021 aagatctcag tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata
    10081 ggcagcc
//
