LOCUS       sequence-46553-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pCol-TGM
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..106)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(103..141)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(174..192,193..215,216..234,235..257,258..276,277..299,
                     300..318,319..341,342..360,361..383,384..402,403..425,
                     426..444)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    456..590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    710..844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggtcaattcg gggccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
       61 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc
      121 ctcccaccgt acacgcctac ctcgacccgg gtaccgagct cgactttcac ttttctctat
      181 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      241 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      301 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
      361 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
      421 acttttctct atcactgata gggagtggta aactcgatcc agacatgata agatacattg
      481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      661 aaaacctcta caaatgtggt atggctgatt atgatcctct agagtcgcag atccagacat
      721 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      781 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      841 agttaacaac aacaattgca ttcattttat gtttcag
//
