LOCUS       sequence-466012-       21127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 eCadh in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21127)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 21127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF5-Elen0515-eCadh-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..21127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      14751..14778
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14910..14937
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(15369..16220)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(16207..17046)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17557..18528)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19767..19854
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      20196..20590
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20753..20796)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20892..21001
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcag
     1501 tcgatttctt gcacgaggtt gatcagctct tgctgggagt gaatccccag cttttggtag
     1561 atgtgctggc gatgggtgga aaccgtgctc ttcgacaggc atagctggtc ttgcacgtac
     1621 ggcaggttgc gtcccttcgc gaacatgatg agaatctcgg tttcgcgttc ggtcagaccg
     1681 taccgatcga ccaccttcag gcatttttcc tggaagcggc gcttgcgttc aagcggaatg
     1741 atgtccatcg ctttcagcag atccgattcg gtgaacacgg cgctgctcac cagcagaatg
     1801 accaaagccg aaaccagcat gatgggaaaa gctgaagcaa gctggatgcc gtgctggtga
     1861 agcacgaacc ggcccagcag catgcccagc agcggtccga ccgcccatgc ggcacgcgcg
     1921 aatgaaaacg tgcgtatcct gaccgacgcg tatcgattgc atacgcccgt cacgatgacc
     1981 cacataaaca agctgaagca ttggtaggcg gccgcattcg cggcgtaggg aaggtaggcc
     2041 gacgaatcgg ggtatacgag aggcagcggc agcagaagcg cgccgatgac gaggaacacg
     2101 acgatcgcgt ggtagcagtt tttcgccatg cgctcggatc ttgagttgag aaggatgagc
     2161 gcgatggcga tcgctccggc ggtcactccg aatgcgacaa gggcctgggt gaacgcgtag
     2221 acggtgcctc cgacgctcgc gatgcccatt tgtatgtaga gggttcggac ggcctcgttc
     2281 gcgaatccca ccaaaagggc gcacagcccg agcctcgtga cgaggtgggc ccagtcgcgt
     2341 cgctcgagcg ggtcttcgat gggcgtctgc tggtcgagcg ggcatggcgc ctcctgcggc
     2401 gccaacccga tgttcttcat gccgaactcg agcaatacgg ctgcgatcag gggcatgctg
     2461 gcggcaaaca cgcatgcctc gaaaggcgag aacagcaaga acagggcgaa aagaagcgtc
     2521 gacaggatgt gggccgatgc ggcgcccgtc acgatggcgc gcatgggcag gaggctgaag
     2581 ctcatgccga atctgatgag gaacagtccc gaaaacacgc ccgacaaaac gcccgccaag
     2641 ctcacgaacg cggtgcccgc gattccctcg aaccccgcaa gcggcataag cagcgtggac
     2701 gcgcacacgc cgaaagggag caggtaaagc gacagcttgg atttcaagaa acgtcccgta
     2761 gccctgtcgg ctataccggc aagcgcaagc gtcaacgtga cggcgatgat ggatatcagg
     2821 taggtggtat cgagcaggct ttcgccgtga tggtagcttg cgaacaggct catgctcttg
     2881 aagctcatcg acacccaggc gatgttgcag cccagcgctg ctcctacggt gatttctgca
     2941 gctgttggga agaccctttt tggattgacc ggctccatgg atttgtcccc atcccccccc
     3001 cgaatgcggg ctttccgccg tccgcaagcg aacggctccg agatgcctat tgtaccgcgc
     3061 tctcgtgccg gtcgagttcc gattgcgaca aaaagactgg gagatttgga aacgcgatca
     3121 gcgtttctcc aggtgaacgg ccatcgtgcg atgcgcacga taccgatttg atcgaaaacg
     3181 tgcgaccagg ccgatgtgcg acggcgacgg gatccatagg gtgaagcgca tcggagagga
     3241 cgatgaaagg aggaggaatg ggcgaactgc tcatgacccg acgtgcgttt gcgaaggtga
     3301 tggccgtgac ggctgcggcc gccggtttca cgggggctca gtcggcgttg gcggataccg
     3361 agccggctgc ctcgtcgggc gaagtcaagc gcatacgctc ggcctgtcgc ggatgcggca
     3421 agatggagtg cggcgtttgg gtgaccgttc aggatgggcg ggttgtcaaa acggagggcg
     3481 atgaaagcgc tttccagtcg gcgggaaacc attgcgcgaa agggcaggcg tccttgcagg
     3541 cggcgtatca tcccgaccgt ctcatgtacc cgctcaagcg cacgaatccc aaagggcaag
     3601 agcccggttg ggtgcgcatc agctgggatg aggcgtatcg atccaccgtc gaggcaatcc
     3661 ataagaacca ggaaaagtac ggcaacgaga cgtgcttctt catgggcggc acgtcgcgta
     3721 tctgggccat gggcccttat ggcgcgctga agcagtgctt cgggtcgccg aacggcatac
     3781 aggccaacga gatatgcaaa ggcccgcgct tctacgctac gaagctgaac gattcgaacg
     3841 cctacagctg gatggaagtg gtggggcgtc cgcgcgtgta cgtgcaatgg ggcggcgcgt
     3901 cggagctgtc caattacgac gatagctgtc gcaccacggt cgacgtggcg acgcgcgccg
     3961 acaagcacat cctggtcgat ccgcgccaga cgaacttggg gaaagaggcg gatatctggg
     4021 tgaaccttcg tcccggaacc gatggggcag tggcgaactg ctgggccaac gtgatcatcg
     4081 agaacgagct gtacgacgat ttgtacgtac ggaagtggat gaacgctccc atgttggtgg
     4141 tggaagatga gagcttcgag ccaacccctt cctcgtcgag cgcgcagacg gccaaggtgc
     4201 gtacgcgcct tctcaaagag tccgacctcg tcgagggcgg ggcggatacc cgcttcatgg
     4261 tgctcaacga gatcacaaac gagctgagct ggtacgatgc cggcggcgac aaccccggct
     4321 gggagggcga ggactgggtt cccgcaaccg agggcaaaga agctcatcag ccgggattgg
     4381 atctgacggg tcagacgcag ggcttcgtgc ttgactacgt accgtttccc gacgggctgc
     4441 ttcccgcgct gcataccccc gaaggcggct tcgaggtgga gttgaaagac ggctccagcg
     4501 tgcacgtgcg cacggtgtgg gagcgctata tcgagttctt ggaagactac actcccgaaa
     4561 aggtgtcgga gatatcgggc gttgacgtcg aagtgctaaa agaggcggcc atcacgtacg
     4621 caacgcgcgt cgacccttcg accgggtacg gcaatggcgg aatccagtac atgctggcgt
     4681 tggaacacgc gtgcaattcg acgcagaaca accgtgcctg cgacctgctg gcgggcataa
     4741 cgggcaacat ggacactccg ggcggcatgc gcggctcgac tcccggttgg cccgtctacg
     4801 acctcggcat gtgcgtgccc gactccggca aaaccaccga gtacaccatc gagaagattt
     4861 tgggcaaaga gcgttttccg atgatcggct cggactgcaa ccccagctgg gcggatgcga
     4921 cgtcggtgta cgacgctatc gagagcggcg agccttataa cgtgacgtgc ggcatcgggc
     4981 aaacgggcga cttcatgaac cagtcgaact ccctctttgc cgcggagcag cttcagaagc
     5041 tcgacttctg gtgctcgatc gatctgtggc acaccccgtg cgtggatatg atggccgata
     5101 tcgcaatgcc cgccgctcat tggctggagc tcgattgcat tcgcaaaagc cagggctcgt
     5161 cgggtgcgtt cggcgctacg gtgaaagcgg tggagcctcc cggagaagcg aagaacgatc
     5221 tggagatcgt ggttggcctg tataaggccg ccggggtccc gtacttcgac gaggagtatc
     5281 acggtgcggc gtggctcgag ggggatgaag ccgtggacgc gtgcaacaac gttgcgctca
     5341 aaagcttccg cattcccgat tggaatgact acaagaagga attccaagag aagggctggt
     5401 tcgattccaa ggtggagaag cccgacgatt ggggcgtcta ccggcgctat caaaccggaa
     5461 acggccacat caacggaggg ttcccgccca atccgaacca gcatcaaggc tggaacacga
     5521 ccacgcacaa gcaggagatc tggtcgacgg tgctggaatc atggttgccc ggcgagggcg
     5581 aggagttccc gaaattcgtg gaggctccgc atggccccgt cgccgacccc gatttgttca
     5641 cggacgacaa ctcgttcttg atgaccaccg ggcgtcgtca aggaacctac ttccattccg
     5701 agcaccggca gctgccatgg tgccgcgagc tgtggcccgt tcctcggctt gagatgaatc
     5761 ccgttgacgc cgagcgactt ggtctcgagc agggggattg ggtatggatc gaaaccgatc
     5821 agcacaagat tcgcgaagtg gtcgatctgt actacggcat cgccccgggt gtggtgaacg
     5881 ccgagcacca atggtggtac cccgagctca atcagcccga tcacgggttc aaattgtccg
     5941 gcgtgaactg tttgatcgat cgccatgccc aagatcgcat catcgggtcg tcgaacctgc
     6001 gtgcttacgg tgtgaaggtg tacaaggcca cgcccgagaa ttcgccgttc ggcaaccccg
     6061 tgccgtgcgg agacgacggg acgcccatca tccacacctg cgacgatccg cgcctgaagg
     6121 aatggcaacc gttgtacgag gggagggagt gacctatggc tcgttatgcg attctgaccg
     6181 atctcaacaa atgcgtcggc tgccttgcgt gctcgatagc gtgcaaggtg gtgaacagcg
     6241 tgccggtggg gtcgtattgg aacaaggtct tgcgcatcgg accgaacccg cgcaccaagg
     6301 gcggccaatg gcccgacgtg tacacctatt tcctcaccgt gcagtgccaa cactgcgaga
     6361 atcccgagtg cgtgaaggtg tgcccgaccg gcgcctctca caagctggag gacggcaccg
     6421 tgcagatcga caagtcgaag tgcatcggct gccagttctg cgcgatgtcg tgcccgtaca
     6481 gcgttcgcta cttgaacgag gaggagggcg tcgtggagaa gtgcacgctg tgcgagcaga
     6541 agatcgccca gggcgagctg ccgcaatgcg tgagcgaatg cggcggtcgc gctcggttct
     6601 tcggcgattt ggaccagggg ctcgagagct ttgaggcgcc ggggtgcgat ctgcaggacc
     6661 cgtcgtacga tgcgcaggcg aaggccagga tgaagtgggg cgatttgata ggcgactcat
     6721 gcgactatcc ggtgaagcct tacgacgaga acgaagtgta ccaccttccc gacgtgggca
     6781 acgcccccag cttcgtctac atcctgcgaa acgagaagtg gcaaggaggc ggtgaatgat
     6841 ggaactccaa tggcctttga ttttgttcac cacgctggtg gcgtggagcg cggggctgtt
     6901 cgggacgcag gctctgatgg cggtgttcgg ggtggggaag cgggcgcagg tcccggcttg
     6961 ggttgcgtcg gcggtgctgc tcgcggccgg cgggatcgcg gtgttcttcc acctggagca
     7021 ctgggagcgg atcttcaacg ggttcgggca cctgacgagc gggatcacgc aggaattgat
     7081 cgcgatcgtg gtgctggcgg tcgtcgcggt cgcgtatctc gtgctcatgc gcaagtccga
     7141 cgacggcgcg agcgtgccga agtggctggc ctgggtcgcc gtcgccttgt cggtggtgct
     7201 cgtggccgtc atggcgcact cgtacacgat ggccgcgagg ccggcgtggg actcggtgct
     7261 ctggatcctc tacgtcctcg gcaacgcctg cgtcctgggc cccgcgactt tcctcctggt
     7321 cttggctgca gggggacccg gggaccagcc cgccgatcgc gcagccgacg ccggggcccc
     7381 cgcaggtagg acggccctcg ccggcgccgc cctcaacgcc ctcgcggccc tcgccttcgc
     7441 gatcttcctc cagctctccg ccggctcctt cgccgacgtc ggcctctact tcgacccgac
     7501 gcaccccacc aaggccatgg ccgacgcagc cgcgacggtc gcctcccagg ccccgctcct
     7561 gtggctgggc gccgtggccg tcggcgcgat cgtccccctg gccgccgcct tcctaggcag
     7621 gcgcacgggg aactggaagc tctgggcccc ggcagccata atcgccgccc tcgtcggagc
     7681 cgtctgcatg cgcgtcgtct tctacaacct ggggctctcg gtcttcatgt tctactagac
     7741 tagtccgctg gttgccacga cgccctcgaa gtctccttcg agggcgttct gtcgtttgca
     7801 agaagggtct ggagaggcaa atcgcgctcg aaggggcttc gaggccgggg taggtcggga
     7861 gggtggaatc cggatgtttc acgtgaaaca tccgttcttt tacggccggt gtttcgcgtg
     7921 aaacattttg cggaaaggac tccttccctt cccgtgcgcc accccaacgc cccgctccta
     7981 gcgctcgtac acctggtcgc agttgagcag atccttcatg gagtgccaga tctggtgcgg
     8041 gttgcccggc gggttcttga gcggcttcat cggatagcgc tcctgcgcgc ccgacagcag
     8101 atcgacggcg aacacttcgt cggacagctc gagcgcgcct tccgggcaga tgtcggtgca
     8161 gcagcggcac ttcacgcaat cgaccggacg atgcgtcacg ccgatcgagc cgtcctcacc
     8221 cgcgtacttc gcgatggccc cggtggggca gaacgtggcg cacatctggc acgacgagca
     8281 cttctcggga tcgatgatca catggcccca caagcgcgtg tccatgagca catcctgcgg
     8341 ctgccccaac gaatcgagcg cggccagcaa acgttcgcgg cggtcgggaa tgaagtgagg
     8401 cagcgtgccg tccttgccca ccttcacgaa cctcggctcc cgccgctcct ccacacccaa
     8461 tgcgtccttg acggcgtagt cagccgtcac cgccgcaacg ttcttagcct cgtccttcat
     8521 gctggagaag aagctccgac ggctcgcgtc gtagccctcg tcgcccgccc ggcgcacgga
     8581 tgagggaagc ttttccgcca ggtcgatgcg catatcgttg ttccaggttg caagcagcgc
     8641 gcttgccgtg tcgcacacaa cccgcgccat ctcgaaaccc gagacatgct cgcattgcgc
     8701 gcagcgcgcc tgcacgaggc tcacgcggcg cacgcccaac gccgccagcg tgaccagcag
     8761 gctctcctcc acgcgcccca ggcacgtgac gcccaccacc ttctcggggt cgtacaaccc
     8821 gtcggccgcg gcaagcagct gatcgcaggc gataaccacc tgtccgtcgg ccacgtcagc
     8881 cgccgcgcgg catgcttgca gaagctcggc atcgttcgga cgatgcgctt caagcgcgca
     8941 ggtgggacac acggtggcgc aggttccgca cccgatgcac cgctcgggtt cgatgaccag
     9001 ctcgttgtcg tcataggaga tgcaaccgct cgtgcacacg tcggcgcact tcatgcacgt
     9061 tgcgttgcga ttgcgcacaa tagcgcaacg accctgatga accgtaatgt cggcgctctg
     9121 aagttgttcg agcagaccga ggaaattcgt cacgtgagcc atgacttcac cctttctcgt
     9181 ctaccccctt gcaccgcaca gtataaccac tttcgccgtc tcacgcgaga aaaaacgcgc
     9241 ttgacacctt cgacaggcct ccctatcatg tggccttggg gagtccgccg gcacgctgat
     9301 cgcgtgcgaa gcggacattg tttgcaaaag tgggaacctc aggggtgagg tttcgcggtg
     9361 ctgtgcgccg taagtgcaaa caatgtctga cgaggggcgg aacatgactc acgattccac
     9421 gaaccagcat ctttctcttg agcccgacta cgacgggttc gatccacttg aagatttcga
     9481 agacgatgcg accgaggtcg aaggccgtgc cgacgagttc gtcgccacgg tgagcgccgc
     9541 cgaagacgcg ctcgctcacg acgcgatggc acccgccacg cccgccatct gcgaaaagcc
     9601 cgctgaagag cgcatcgccg acttgttgga ggccatggcg ccgcgccgca cggtgctgct
     9661 gggcatcctg gccgcctgcg ccgagccgca gccggcatcc gccgtgaacg ctcgcgtcga
     9721 cgagctgcag gagaacaact tctcggtgta cagcgcggcc aacctgtgct ccttgctgga
     9781 gaaggcgggc gccatcgagc gcgtcacggc cgagggcgaa cccgcggagg acatcgaaat
     9841 cgagccacag accgtcgtcg tcgacggcgt cgaatacctg gaagcccgcg aacccgtcga
     9901 gatctactgg cacatcaccg agccgggccg cgcggcgctt gaggccgaca aaccgctcga
     9961 gcgcctgcgc gccctcctgg acgaagatgc cgcctacgcg cccatctacc agcgcatcct
    10021 ccgcttgtgc acagcagacg gcggcgccac gacacccgcc atcaacaacg cggtcgacca
    10081 caacccgctg gtgcagaagc cgcgcttcta cgcgccccac ttcgtcgacc gcctggagaa
    10141 atgcgacgcg ctggcctgga agaaggcgtg gtgcatcacc gacatcggac gcgccggcct
    10201 cgatatgctg gcagacgtcg tagacgagaa cgcgcccgcc acccagcccg aaacccccgc
    10261 aacccctgat cccgccgcat ccaaggagga ctaaccatga ccgaagcaac cgcgcccgca
    10321 accgagaccc tcgccgacga agcgaccatg caggagctcg tcaaactgaa cgagcagcgc
    10381 gccgccacgt acggcctgct gtcgcgcctg taccgcgtgg aggtggacca gccccttctc
    10441 gacgagctgc gcagcatgcg cttccccgcc aagacgggca acgagaacgt ggacgagggc
    10501 taccgcctgc tggccacgta cctgagcaac acctgggaca acagcgtcac cgacctcgcc
    10561 atcgactacg tgcgcgtgtt catcggacac ggcatcgacg cgttctcggc cgcctacccg
    10621 ttcgaatcgg tgtacacctc cgagaagcgc ctgctcatgc aggaagcacg cgacgaggtg
    10681 ctggccatct accgcagcgc aggcctcgac aagcaggaca catggaaaga gggcgaggat
    10741 catatcgcgc tcgagctgga attcgagcag atcatggcca cgcgcacggt cgaagcgctg
    10801 cgcaaaggcg acgaagacga ggccgtcgcg ctgctgacca cgcagaagaa ctttctcgaa
    10861 gaccatctgg cggcatgggc tcccatgatg acctccgaca tgaagcgatt cgcgcaaacc
    10921 gacttctacc ggggcttggc gtacttgacg gacgggttcc tccaaaccga ccaaacgttc
    10981 ctcgacgacg tgctggccga agactaactg cacaggatcg aaagaaggag cgatggacaa
    11041 caagcaggag aaaacctacg ccggcatgac gcgacgcacg ctgtgcctgg gcgcaggcgg
    11101 cgctgcggtc atgctaggtc taggcggatt gaagtacgcg ggatcgcaac cgctcgtgcg
    11161 cccgccggga ggccaggacg aggaacggct catctcggcc tgcatccgct gcgagaagtg
    11221 ctacgagatc tgcccgcgcg acgtcatcgc gcccgcccac ctcgaggacg gtgtgctgaa
    11281 catgcgcacc cccacgttcg acttctcggc caactggtgc gactggtgcg ccgaagagca
    11341 cgacggcacc ccgttgtgcg tgagcgcctg ccccaccgaa gcgctcaagc tgcccgacga
    11401 cgccacggcc gagaacacca tcatcggcaa agccgttatc aacacggact ggtgcttggc
    11461 gtacaagctg atcggatgcc ggttttgcta cgacgcctgt ccctacgagg ccatggagct
    11521 cgacgagaac aaccgccccg tcgtgatcga ggacaagtgc aacggctgcg gcgcgtgcga
    11581 gagcgtgtgc gtctcgctgc agaacggctc catctccgaa ggagccaccg cgcgcgccat
    11641 cgtcgtccac ccgcttgacg aagttgaagg ataaggtgcc ctcatgaaga agaactccaa
    11701 gaagctgcgc accctcgtcg cactggccat cgtggccgtc gtcgccgtcg gcttcgtcac
    11761 gaacgtcggg atcggcacca tgtcggcacc gggcatctgg gacatctcca tcctatgccc
    11821 gctgggcgcc ttgggaacga tgctcgcctc caagatgatg gtgccgcgcg ccgtaatctc
    11881 gctgatcgtg atggtgctgc tcatcgttct gttcgcccgc gcgttctgcg gatggatttg
    11941 ccccgtgccg ctcgtgcaga agttgcgcgg cttgttcgcg aagaagcaac ccgtcgaacg
    12001 cggcgacgat gccgatcctg ccgcggcgcc gctcacgaaa gtcgagatcg acgcgctggg
    12061 cgtttcatgc tcgaaggggt cgaagaatgg cagcggatgc gcgtcctgcg cctccaagcg
    12121 cggagccgcg ctcgatgctc gccacttcgt gctgggcggc gcgctggcgt ccaccttcat
    12181 ctttggattc ccggtgttct gcctcgtctg ccccatcgga ctcacgttcg ccaccatcct
    12241 gctggtcatc aacctgttct cacacggcga tgtcacctgg tcgctcatcg tggtgcccgc
    12301 gctgcttatc gccgaggtgc tgctgttcaa gaagtggtgc cataagctgt gcccgctttc
    12361 ggcgttcatg agcctgatcg cgaaggcgaa caagaccttc gtgcccacga tcgacgatgc
    12421 gaagtgcctg gaaacggcga agggcggcgc gtgcggggcc tgcggccgcg tctgcgagga
    12481 gggcatcgac ccgcgccacc cgcagctttc gcaggcggca tggagcgagt gcaccaagtg
    12541 ccgcgcctgc gtggacgcat gccctgcgaa cgcgatcacc atgccgctgc tgccgcggcg
    12601 aacgccgtct accgcagaac ctgtcgccgt gccaacgaac gccgagaaga tcgacgcgga
    12661 gaatgcgcgc cccgttccgt aaagcccctc ctgcgcgacc cggctcgcag ctgcagcggt
    12721 ctggtcgcgc atccggaatc gggccgagcc cgaccccggg caaaaggcgg cttggcaagg
    12781 gctttggggg acgagccgcc ttttgcccgg gagcgacatc cccgctcccc ctccctgcga
    12841 aaagagggat gccgcttccg gccttcatct gacaaccacg acgtgggagg acgaaaaccc
    12901 atggcatacg aaacgacaag cgaatcgacc gaggccctgc tcgtcaccgg aggcgtcggc
    12961 accggcaaaa cgcaacgtct cgtcgagcgc gctgccgcgc tgctggccga aaacgccgcc
    13021 gcgggcaacg tgctcgtgct gtgcgcgacg ccccaggccg cacgcctgtt ctcggagcgg
    13081 ttgacagccc gcgtcggtgc caagcgcgct gccggcgcaa ccgtcgccac cgcacgcgcc
    13141 ttagcgctcg acgtgctggc cgacgacggc gcgcgccgct ggtcggggcg cgagccgcga
    13201 ctactgaccg ccttcgaaga gacgttcctc atggaggaca tgaaggtgag cgggctgcgg
    13261 ccgaagcggc tgcgcgagat gctcaagttc ttctaccgct cgtggaccga gctggcggac
    13321 gacgatcccg attggctgct accgggcgag gaggccgatg tccatacgct gttgaaagag
    13381 aacctggtct tcatgcgcgc cgtcatagaa cccgaagcgg cgaaccttgc tgcacgctac
    13441 ctccgagaac acgacgaagc gcgggcggcg cattcctacg cgcacgtgct gatggacgac
    13501 tacgcctgcg caagccgtgc ctcgcagacg ctggcgaatc tcgtcgcaac ctgctcgatc
    13561 accgtggcgg gcgacgcgga aacctgcatc gagacatacg attcgtaccc ctatgccgcc
    13621 ggaatcgacg aattcctcgc aacgcatccc gatgctgctt gcgagcacct ggatgccgac
    13681 gcgcccgatg ccgtcgaacg ggtcgctctg gcaagcgaca cgcccgaagc cgagttcgaa
    13741 caggtggcgc gcacggtggc cgaagccgtc gccgcaggaa cgcccgcacg gcagatcgtc
    13801 gtggccgtac cgaacagcgt gtggtcgcgc aacatcgcaa ccgctttgca cgcacgaggc
    13861 gtgccaaccg aagcgctcgc catctcgcag cccctgcgcg gcgacgtgcg ggacaacgcc
    13921 cgctgcacgc ctgcgcgcat cgccacggcc ctcaacctcg tggcgaaccc agacgacacc
    13981 gcggcttggc ggtgctggtg tggattcggc gactacctgg tgaacagcgc ggctttcgcc
    14041 agcctgcgga cttgcgcgaa ggagcgcagt atcggcctcg tggacgcttt ggagatgcta
    14101 agcgaaaacg actgcgagca cgtagtgggc gcgcaacgcg tggcgaccgc ctacaaggac
    14161 ggtcgcgcgc tcatcgagca ggtacgagga ttgcggggca cggcactgct ggacgagctg
    14221 acgcgcatcg tcaccgacgg agcggaatcc caagcgcccg ccgttctgcg cgagctctgc
    14281 ctcgagcacg gcaacgcagc cgatgaaaac ccggaaagcc tttacgatgc cgatgctgcg
    14341 gcgatggcgc ggcgcatggc cgagcgcctg ctcgcgccca cgctggaggt tgccgacgcg
    14401 gtagccgtcg tcccctacga cttcgcctgc ggcttctcgc ccgacctgct gatcgtcgcc
    14461 ggcttcgtga acggtttcat cccctcccgc gactatttcg acgccacggt gaccccgccc
    14521 gagcgccagg agaagatgcg cgccgtcgac gaacgacgcg tgcgcgcgct cacaggaaag
    14581 gccggccgcg cggtggcgct gtcgtacttc acgtccaccg acctcgaaag cgccggcaag
    14641 ctgaagctca agatcagtcg catccgcctc aagaacggta tccgcacctg cactatagag
    14701 cccagcctat tcctggagca aatcgcagcg caggagtagc gactcacact ggctcacctt
    14761 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat
    14821 ccggcttttt tattatttaa ttcctgttat aaaaaaagga tcaattttga actctctccc
    14881 aaagttgatc ccttaacgat ccctgcagga gaaggccatc ctgacggatg gcctttttga
    14941 agctttctag aatcgggggc ccccgggggg attctagaac aatggaagtc ctcgtctcgc
    15001 cctcgaatta gcccgcttaa tgagcgggct ttttttgaat taattctcgc gagctggcac
    15061 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagcgc
    15121 gaattgcaag ctggccgacg cgctgggcta cgtcttgctg gcgttcggga gcagaagagc
    15181 atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag
    15241 gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca
    15301 attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt
    15361 ggggggggtc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg
    15421 gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc
    15481 ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg
    15541 gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg
    15601 gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca
    15661 cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg
    15721 tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg
    15781 atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta
    15841 tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca
    15901 tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg
    15961 gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc
    16021 aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg
    16081 tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca
    16141 cggaacaggc cgggggccag acagtgcgcc gggtcgtgtc ggacgtggct gaggctgtgc
    16201 ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc
    16261 gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata
    16321 gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca
    16381 ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac
    16441 cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc
    16501 cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat
    16561 gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg
    16621 cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca
    16681 ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc
    16741 cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat
    16801 ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac
    16861 ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt
    16921 accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac
    16981 gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt
    17041 agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc
    17101 ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg
    17161 gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct
    17221 tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc
    17281 atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa
    17341 ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc
    17401 cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag
    17461 gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta
    17521 ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc
    17581 cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg
    17641 gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc
    17701 gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc
    17761 actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc
    17821 accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg
    17881 gtggcggcta agctgccgct cggacagttc gaggctggcc agcctgcggg ccttctcctg
    17941 ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt
    18001 ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt
    18061 gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct
    18121 ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc
    18181 gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac
    18241 cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc
    18301 caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg
    18361 ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg
    18421 gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat
    18481 ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag
    18541 cgcagccaga tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat
    18601 gcgatcagca gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc
    18661 cagtgccacc cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct
    18721 acctcccgca actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc
    18781 agccactccg ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct
    18841 tcgtcggcca gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg
    18901 atgcctctgg atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc
    18961 ctgtaagtcg atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc
    19021 ggtgcggatg ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag
    19081 gcgctcgatg ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggcctgcccg
    19141 ctctaatgcc cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt
    19201 gggcttgagc gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg
    19261 cttgaaccac tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag
    19321 gtggcagtgc gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgctc
    19381 ggcaccggtc aggtgctggg cgaactcgga cgccagcgcc ttctgctggt cgagggtcag
    19441 ctcgaccggc agggcaaatt cgacctcctt gaacagccgc ccattggcgc gttcatacag
    19501 gtcggcagca tcccagtagt cggcgggccg ctcgacgaac tccggcatgt gcccggattc
    19561 ggcgtgcaag acttcatcca tgtcgcgggc atacttgcct tcgcgctgga tgaagtcggc
    19621 cttggccctg gccgattggc cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat
    19681 cgccatgctg cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc ctcggagagc
    19741 gcaccgcccg aagggtggcc gttaggccag tttctcgaag agaaaccggt aagtgcgccc
    19801 tcccctacaa agtagggtcg ggattgccgc cgctgtgcct ccatgatagc ctacgagaca
    19861 gcacattaac aatggggtgt caagatggtt aaggggagca acaaggcggc ggatcggctg
    19921 gccaagctcg aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg
    19981 gaacagcagc aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt
    20041 ttggccaagg tgaacagcag cgagtggccg gaggatcggc tcatggcggc aatggatgcg
    20101 taccttgaac gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag
    20161 ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc
    20221 gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt
    20281 ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg
    20341 tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag
    20401 ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc
    20461 atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg
    20521 ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg
    20581 caggggttcg tgacagttag tacgggagtg acgggcactg gctggcaatg tctagcaacg
    20641 gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg tatgtaaaca
    20701 cagtattgca aggacgcgga acatgcctca tgtggcggcc ctcgcgagaa ttaattcaga
    20761 taaaaaaaat ccttagcttt cgctaaggat gatttctagc gatgaccctg ctgattggtt
    20821 cgctgaccgt agactggttg gcttggtttc atcagccatc cgcttgccct catctgttac
    20881 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
    20941 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
    21001 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
    21061 atatcgtgcg acatcgcgcg cgcgcgcgcg gatgctagcg gcgcgccaaa aaaaagcccg
    21121 ctcatta
//
