LOCUS       sequence-466128-        1677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1677)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3_GMCL1-FLAG
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1588..1611
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1627..1650
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agacccaagc ttggtaccgt gtgctggatg ggatcgttga gcagccgggt gctgcgccag
       61 ccaagaccag cccttgccca gcaggcgcag ggtgccaggg cggggggctc ggcccggagg
      121 ccggacactg gagacgatgc ggcgggccac ggattctgtt actgtgcggg cagccacaag
      181 cgcaagcgga gcagcgggtc cttctgctac tgtcaccctg actcggagac ggacgaggat
      241 gaggaggagg gggacgagca gcagcggctc ctcaacaccc ctcgaaggaa aaaattaaag
      301 agtacatcta aatatattta tcaaacatta tttttgaatg gtgaaaacag tgacattaag
      361 atttgtgctc taggagaaga atggagctta cacaaaatat atttatgtca atctggctac
      421 ttttctagta tgttcagtgg ttcttggaaa gaatccagca tgaatattat tgaactggag
      481 attcctgacc agaacattga tgtagaagca ctgcaggttg catttggttc actgtatcga
      541 gatgatgtct tgataaagcc cagtcgagtt gttgccattt tggcagcagc ttgtttgctg
      601 cagttggacg gtttaataca gcagtgtggt gagacaatga aggaaacagt taatgtgaaa
      661 actgtatgtg gctattacac atcagcaggg acctatggat tagattctgt aaagaaaaag
      721 tgccttgaat ggcttctaaa caatttgatg actcaccaga atgttgaact ttttaaagaa
      781 ctcagtataa atgtcatgaa acagctcatt ggttcatcta acttatttgt gatgcaagtg
      841 gagatggata tatacactgc tctaaaaaag tggatgttcc ttcaacttgt gccttcttgg
      901 aatggatctt taaaacagct tttgacagaa acagatgtct ggttttctaa acagaggaaa
      961 gattttgaag gtatggcctt tcttgaaact gaacaaggaa aaccatttgt gtcagtattc
     1021 agacatttaa ggttacaata tattatcagt gatctggctt ctgcaagaat tattgaacaa
     1081 gatgctgtag taccttcaga atggctctct tctgtgtata aacagcagtg gtttgctatg
     1141 ctgcgggcag aacaggacag tgaggtgggg cctcaagaaa tcaataaaga agaactagag
     1201 ggaaacagca tgaggtgtgg tagaaagctt gccaaagatg gtgaatactg ctggcgttgg
     1261 acaggtttta acttcggctt cgacctactt gtaacttaca ccaatcgata catcattttc
     1321 aaacgcaata cactgaatca gccatgtagc ggatctgtca gtttacagcc tcgaaggagc
     1381 atagcattta gattacgttt ggcttctttt gatagtagtg gaaaactaat atgtagtaga
     1441 acaactggct atcaaatact tacacttgaa aaggatcagg aacaagtggt gatgaacttg
     1501 gacagcaggc ttctgatctt ccctttatat atctgctgta acttcttgta tatatcacca
     1561 gaaaaaaaga atggaggatc aggaatggac tacaaggacg acgatgacaa ggtaccagga
     1621 tctatggatt ataaagatga tgatgataaa gggtcggccg ccagctaggg atccact
//
