LOCUS       sequence-466132-        6435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6435)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_53BP1IEDI_AAAA_FLAG
FEATURES             Location/Qualifiers
     source          1..6435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          21..117
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             211..234
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             250..273
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6258..6392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6282..6301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6336..6355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaaaaccaga aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat
       61 ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc
      121 tgtacggaag tgttacttct gctctaaaag ctgcggaatt gtacccgcgg gcccaccatg
      181 tggctagcgt ttaaacttaa gcttaccatg gactacaagg acgacgatga caaggtacca
      241 ggatctgggg attataaaga tgatgatgat aaagggtcgg ccgccagcct tatggccatg
      301 gaggcccgaa ttcggtcgac aatggaccct actggaagtc agttggattc agatttctct
      361 cagcaagata ctccttgcct gataattgaa gattctcagc ctgaaagcca ggttctagag
      421 gatgattctg gttctcactt cagtatgcta tctcgacacc ttcctaatct ccagacgcac
      481 aaagaaaatc ctgtgttgga tgttgtgtcc aatcctgaac aaacagctgg agaagaacga
      541 ggagacggta atagtgggtt caatgaacat ttgaaagaaa acaaggttgc agaccctgtg
      601 gattcttcta acttggacac atgtggttcc atcagtcagg tcattgagca gttacctcag
      661 ccaaacagga caagcagtgt tctgggaatg tcagtggaat ctgctcctgc tgtggaggaa
      721 gagaagggag aagagttgga acagaaggag aaagagaagg aagaagatac ttcaggcaat
      781 actacacatt cccttggtgc tgaagatact gcctcatcac agttgggttt tggggttctg
      841 gaactctccc agagccagga tgttgaggaa aatactgtgc catatgaagt ggacaaagag
      901 cagctacaat cagtaaccac caactctggt tataccaggc tgtctgatgt ggatgctaat
      961 actgcaatta agcatgaaga acagtccaac gaagatatcc ccatagcaga acagtccagc
     1021 aaggacatcc ctgtgacagc acagcccagt aaggatgtac atgttgtaaa agagcaaaat
     1081 ccaccacctg caaggtcaga ggacatgcct tttagcccca aagcatctgt tgctgctatg
     1141 gaagcaaaag aacagttgtc tgcacaagaa cttatggaaa gtggactgca gattcagaag
     1201 tcaccagagc ctgaggtttt gtcaactcag gaagacttgt ttgaccagag caataaaaca
     1261 gtatcttctg atggttgctc tactccttca agggaggaag gtgggtgttc tttggcttcc
     1321 actcctgcca ccactctgca tctcctgcag ctctctggtc agaggtccct tgttcaggac
     1381 agtctttcca cgaattcttc agatcttgtt gctccttctc ctgatgcttt ccgatctact
     1441 ccttttatcg ttcctagcag tcccacagag caagaaggga gacaagataa gccaatggac
     1501 acgtcagtgt tatctgaaga aggaggagag ccttttcaga agaaacttca aagtggtgaa
     1561 ccagtggagt tagaaaaccc ccctctcctg cctgagtcca ctgtatcacc acaagcctca
     1621 acaccaatat ctcagagcac accagtcttc cctcctgggt cacttcctat cccatcccag
     1681 cctcagtttt ctcatgacat ttttattcct tccccaagtc tggaagaaca atcaaatgat
     1741 gggaagaaag atggagatat gcatagttca tctttgacag ttgagtgttc taaaacttca
     1801 gagattgaac caaagaattc ccctgaggat cttgggctat ctttgacagg ggattcttgc
     1861 aagttgatgc tttctacaag tgaatatagt cagtccccaa agatggagag cttgagttct
     1921 cacagaattg atgaagatgg agaaaacaca cagattgagg atacggaacc catgtctcca
     1981 gttctcaatt ctaaatttgt tcctgctgaa aatgatagta tcctgatgaa tccagcacag
     2041 gatggtgaag tacaactgag tcagaatgat gacaaaacaa agggagatga tacagacacc
     2101 agggatgaca ttagtatttt agccactggt tgcaagggca gagaagaaac ggtagcagaa
     2161 gatgtttgta ttgatctcac ttgtgattcg gggagtcagg cagttccgtc accagctact
     2221 cgatctgagg cactttctag tgtgttagat caggaggaag ctatggaaat taaagaacac
     2281 catccagagg aggggtcttc agggtctgag gtggaagaaa tccctgagac accttgtgaa
     2341 agtcaaggag aggaactcaa agaagaaaat atggagagtg ttccgttgca cctttctctg
     2401 actgaaactc agtcccaagg gttgtgtctt caaaaggaaa tgccaaaaaa agaatgctca
     2461 gaagctatgg aagttgaaac cagtgtgatt agtattgatt cccctcaaaa gttggcaata
     2521 cttgaccaag aattggaaca taaggaacag gaagcttggg aagaagctac ttcagaggac
     2581 tccagtgttg tcattgtaga tgtgaaagag ccatctccca gagttgatgt ttcttgtgaa
     2641 cctttggagg gagtggagaa gtgctcagat tcccagtcat gggaggatat tgctccagaa
     2701 atagaaccat gtgctgagaa tagattagac accaaggaag aaaagagtgt agaatatgaa
     2761 ggagatctga aatcagggac tgcagaaaca gaacctgtag agcaagattc ttcacagcct
     2821 tccttacctt tagtgagagc agatgatcct ttaagacttg accaggagtt gcagcagccc
     2881 caaactcagg agaaaacaag taattcatta acagaagact caaaaatggc taatgcaaag
     2941 cagctaagct cagatgcaga ggcccagaag ctggggaagc cctctgccca tgcctcacaa
     3001 agcttctgtg aaagttctag tgaaacccca tttcatttca ctttgcctaa agaaggtgat
     3061 atcatcccac cattgactgg tgcaacccca cctcttattg ggcacctaaa attggagccc
     3121 aagagacaca gtactcctat tggtattagc aactatccag aaagcaccat agcaaccagt
     3181 gatgtcatgt ctgaaagcat ggtggagacc catgatccca tacttgggag tggaaaaggg
     3241 gattctgggg ctgccccaga cgtggatgat aaattatgtc taagaatgaa actggttagt
     3301 cctgagactg aggcgagtga agagtctttg cagttcaacc tggaaaagcc tgcaactggt
     3361 gaaagaaaaa atggatctac tgctgttgct gagtctgttg ccagtcccca gaagaccatg
     3421 tctgtgttga gctgtatctg tgaagccagg caagagaatg aggctcgaag tgaggatccc
     3481 cccaccacac ccatcagggg gaacttgctc cactttccaa gttctcaagg agaagaggag
     3541 aaagaaaaat tggagggtga ccatacaatc aggcagagtc aacagcctat gaagcccatt
     3601 agtcctgtca aggaccctgt ttctcctgct tcccagaaga tggtcataca agggccatcc
     3661 agtcctcaag gagaggcaat ggtgacagat gtgctagaag accagaaaga aggacggagt
     3721 actaataagg aaaatcctag taaggccttg attgaaaggc ccagccaaaa taacatagga
     3781 atccaaacca tggagtgttc cttgagggtc ccagaaactg tttcagcagc aacccagact
     3841 ataaagaatg tgtgtgagca ggggaccagt acagtggacc agaactttgg aaagcaagat
     3901 gccacagttc agactgagag ggggagtggt gagaaaccag tcagtgctcc tggggatgat
     3961 acagagtcgc tccatagcca gggagaagaa gagtttgata tgcctcagcc tccacatggc
     4021 catgtcttac atcgtcacat gagaacaatc cgggaagtac gcacacttgt cactcgtgtc
     4081 attacagatg tgtattatgt ggatggaaca gaagtagaaa gaaaagtaac tgaggagact
     4141 gaagagccaa ttgtagagtg tcaggagtgt gaaactgaag tttccccttc acagactggg
     4201 ggctcctcag gtgacctggg ggatatcagc tccttctcct ccaaggcatc cagcttacac
     4261 cgcacatcaa gtgggacaag tctctcagct atgcacagca gtggaagctc agggaaagga
     4321 gccggaccac tcagagggaa aaccagcggg acagaacccg cagattttgc cttacccagc
     4381 tcccgaggag gcccaggaaa actgagtcct agaaaagggg tcagtcagac agggacgcca
     4441 gtgtgtgagg aggatggtga tgcaggcctt ggcatcagac agggagggaa ggctccagtc
     4501 acgcctcgtg ggcgtgggcg aaggggccgc ccaccttctc ggaccactgg aaccagagaa
     4561 acagctgtgc ctggcccctt gggcgcagcg gccgcttcac ctaacttgtc accagatgat
     4621 aaatccttca gccgtgtcgt gccccgagtg ccagactcca ccagacgaac agatgtgggt
     4681 gctggtgctt tgcgtcgtag tgactctcca gaaattcctt tccaggctgc tgctggccct
     4741 tctgatggct tagatgcctc ctctccagga aatagctttg tagggctccg tgttgtagcc
     4801 aagtggtcat ccaatggcta cttttactct gggaaaatca cacgagatgt cggagctggg
     4861 aagtataaat tgctctttga tgatgggtac gaatgtgatg tgttgggcaa agacattctg
     4921 ttatgtgacc ccatcccgct ggacactgaa gtgacggccc tctcggagga tgagtatttc
     4981 agtgcaggag tggtgaaagg acataggaag gagtctgggg aactgtacta cagcattgaa
     5041 aaagaaggcc aaagaaagtg gtataagcga atggctgtca tcctgtcctt ggagcaagga
     5101 aacagactga gagagcagta tgggcttggc ccctatgaag cagtaacacc tcttacaaag
     5161 gcagcagata tcagcttaga caatttggtg gaagggaagc ggaaacggcg cagtaacgtc
     5221 agctccccag ccacccctac tgcctccagt agcagcagca caacccctac ccgaaagatc
     5281 acagaaagtc ctcgtgcctc catgggagtt ctctcaggca aaagaaaact tatcacttct
     5341 gaagaggaac ggtcccctgc caagcgaggt cgcaagtctg ccacagtaaa acctggtgca
     5401 gtaggggcag gagagtttgt gagcccctgt gagagtggag acaacaccgg tgaaccctct
     5461 gccctggaag agcagagagg gcctttgcct ctcaacaaga ccttgtttct gggctacgca
     5521 tttctcctta ccatggccac aaccagtgac aagttggcca gccgctccaa actgccagat
     5581 ggtcctacag gaagcagtga agaagaggag gaatttttgg aaattcctcc tttcaacaag
     5641 cagtatacag aatcccagct tcgagcagga gctggctata tccttgaaga tttcaatgaa
     5701 gcccagtgta acacagctta ccagtgtctt ctaattgcgg atcagcattg tcgaacccgg
     5761 aagtacttcc tgtgccttgc cagtgggatt ccttgtgtgt ctcatgtctg ggtccatgat
     5821 agttgccatg ccaaccagct ccagaactac cgtaattatc tgttgccagc tgggtacagc
     5881 cttgaggagc aaagaattct ggactggcaa ccccgtgaaa atcctttcca gaatctgaag
     5941 gtactcttgg tatcagacca acagcagaac ttcctggagc tctggtctga gatcctcatg
     6001 actggtggtg cagcctctgt gaagcagcac cattcaagtg cccataacaa agatattgct
     6061 ttaggggtat ttgatgtggt ggtgacggac ccctcatgcc cagcctcggt gctgaagtgt
     6121 gctgaagcat tgcagctgcc tgtggtgtca caagagtggg tgatccagtg cctcattgtt
     6181 ggggagagaa ttggattcaa gcagcatcca aaatataaac acgattatgt ttctcactaa
     6241 ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     6301 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6361 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6421 ttcaggttca ggggg
//
