LOCUS       sequence-466133-        4953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4953)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_53BP1DeltaMFF_FLAG
FEATURES             Location/Qualifiers
     source          1..4953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          21..117
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             211..234
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             250..273
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4776..4910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4800..4819)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4854..4873
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaaaaccaga aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat
       61 ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc
      121 tgtacggaag tgttacttct gctctaaaag ctgcggaatt gtacccgcgg gcccaccatg
      181 tggctagcgt ttaaacttaa gcttaccatg gactacaagg acgacgatga caaggtacca
      241 ggatctgggg attataaaga tgatgatgat aaagggtcgg ccgccagcct tatggccatg
      301 gaggcccgaa ttcggtcgac aatggaccct actggaagtc agttggattc agatttctct
      361 cagcaagata ctccttgcct gataattgaa gattctcagc ctgaaagcca ggttctagag
      421 gatgattctg gttctcactt cagtatgcta tctcgacacc ttcctaatct ccagacgcac
      481 aaagaaaatc ctgtgttgga tgttgtgtcc aatcctgaac aaacagctgg agaagaacga
      541 ggagacggta atagtgggtt caatgaacat ttgaaagaaa acaaggttgc agaccctgtg
      601 gattcttcta acttggacac atgtggttcc atcagtcagg tcattgagca gttacctcag
      661 ccaaacagga caagcagtgt tctgggaatg tcagtggaat ctgctcctgc tgtggaggaa
      721 gagaagggag aagagttgga acagaaggag aaagagaagg aagaagatac ttcaggcaat
      781 actacacatt cccttggtgc tgaagatact gcctcatcac agttgggttt tggggttctg
      841 gaactctccc agagccagga tgttgaggaa aatactgtgc catatgaagt ggacaaagag
      901 cagctacaat cagtaaccac caactctggt tataccaggc tgtctgatgt ggatgctaat
      961 actgcaatta agcatgaaga acagtccaac gaagatatcc ccatagcaga acagtccagc
     1021 aaggacatcc ctgtgacagc acagcccagt aaggatgtac atgttgtaaa agagcaaaat
     1081 ccaccacctg caaggtcaga ggacatgcct tttagcccca aagcatctgt tgctgctatg
     1141 gaagcaaaag aacagttgtc tgcacaagaa cttatggaaa gtggactgca gattcagaag
     1201 tcaccagagc ctgaggtttt gtcaactcag gaagacttgt ttgaccagag caataaaaca
     1261 gtatcttctg atggttgctc tactccttca agggaggaag gtgggtgttc tttggcttcc
     1321 actcctgcca ccactctgca tctcctgcag ctctctggtc agaggtccct tgttcaggac
     1381 agtctttcca cgaattcttc agatcttgtt gctccttctc ctgatgcttt ccgatctact
     1441 ccttttatcg ttcctagcag tcccacagag caagaaggga gacaagataa gccaatggac
     1501 acgtcagtgt tatctgaaga aggaggagag ccttttcaga agaaacttca aagtggtgaa
     1561 ccagtggagt tagaaaaccc ccctctcctg cctgagtcca ctgtatcacc acaagcctca
     1621 acaccaatat ctcagagcac accagtcttc cctcctgggt cacttcctat cccatcccag
     1681 cctcagtttt ctcatgacat ttttattcct tccccaagtc tggaagaaca atcaaatgat
     1741 gggaagaaag atggagatat gcatagttca tctttgacag ttgagtgttc taaaacttca
     1801 gagattgaac caaagaattc ccctgaggat cttgggctat ctttgacagg ggattcttgc
     1861 aagttgatgc tttctacaag tgaatatagt cagtccccaa agatggagag cttgagttct
     1921 cacagaattg atgaagatgg agaaaacaca cagattgagg atacggaacc catgtctcca
     1981 gttctcaatt ctaaatttgt tcctgctgaa aatgatagta tcctgatgaa tccagcacag
     2041 gatggtgaag tacaactgag tcagaatgat gacaaaacaa agggagatga tacagacacc
     2101 agggatgaca ttagtatttt agccactggt tgcaagggca gagaagaaac ggtagcagaa
     2161 gatgtttgta ttgatctcac ttgtgattcg gggagtcagg cagttccgtc accagctact
     2221 cgatctgagg cactttctag tgtgttagat caggaggaag ctatggaaat taaagaacac
     2281 catccagagg aggggtcttc agggtctgag gtggaagaaa tccctgagac accttgtgaa
     2341 agtcaaggag aggaactcaa agaagaaaat atggagagtg ttccgttgca cctttctctg
     2401 actgaaactc agtcccaagg gttgtgtctt caaaaggaaa tgccaaaaaa agaatgctca
     2461 gaagctatgg aagttgaaac cagtgtgatt agtattgatt cccctcaaaa gttggcaata
     2521 cttgaccaag aattggaaca taaggaacag gaagcttggg aagaagctac ttcagaggac
     2581 tccagtgttg tcattgtaga tgtgaaagag ccatctccca gagttgatgt ttcttgtgaa
     2641 cctttggagg gagtggagaa gtgctcagat tcccagtcat gggaggatat tgctccagaa
     2701 atagaaccat gtgctgagaa tagattagac accaaggaag aaaagagtgt agaatatgaa
     2761 ggagatctga aatcagggac tgcagaaaca gaacctgtag agcaagattc ttcacagcct
     2821 tccttacctt tagtgagagc agatgatcct ttaagacttg accaggagtt gcagcagccc
     2881 caaactcagg agaaaacaag taattcatta acagaagact caaaaatggc taatgcaaag
     2941 cagctaagct cagatgcaga ggcccagaag ctggggaagc cctctgccca tgcctcacaa
     3001 agcttctgtg aaagttctag tgaaacccca tttcatttca ctttgcctaa agaaggtgat
     3061 atcatcccac cattgactgg tgcaacccca cctcttattg ggcacctaaa attggagccc
     3121 aagagacaca gtactcctat tggtattagc aactatccag aaagcaccat agcaaccagt
     3181 gatgtcatgt ctgaaagcat ggtggagacc catgatccca tacttgggag tggaaaaggg
     3241 gattctgggg ctgccccaga cgtggatgat aaattatgtc taagaatgaa actggttagt
     3301 cctgagactg aggcgagtga agagtctttg cagttcaacc tggaaaagcc tgcaactggt
     3361 gaaagaaaaa atggatctac tgctgttgct gagtctgttg ccagtcccca gaagaccatg
     3421 tctgtgttga gctgtatctg tgaagccagg caagagaatg aggctcgaag tgaggatccc
     3481 cccaccacac ccatcagggg gaacttgctc cactttccaa gttctcaagg agaagaggag
     3541 aaagaaaaat tggagggtga ccatacaatc aggcagagtc aacagcctat gaagcccatt
     3601 agtcctgtca aggaccctgt ttctcctgct tcccagaaga tggtcataca agggccatcc
     3661 agtcctcaag gagaggcaat ggtgacagat gtgctagaag accagaaaga aggacggagt
     3721 actaataagg aaaatcctag taaggccttg attgaaaggc ccagccaaaa taacatagga
     3781 atccaaacca tggagtgttc cttgagggtc ccagaaactg tttcagcagc aacccagact
     3841 ataaagaatg tgtgtgagca ggggaccagt acagtggacc agaactttgg aaagcaagat
     3901 gccacagttc agactgagag ggggagtggt gagaaaccag tcagtgctcc tggggatgat
     3961 acagagtcgc tccatagcgc cctggaagag cagagagggc ctttgcctct caacaagacc
     4021 ttgtttctgg gctacgcatt tctccttacc atggccacaa ccagtgacaa gttggccagc
     4081 cgctccaaac tgccagatgg tcctacagga agcagtgaag aagaggagga atttttggaa
     4141 attcctcctt tcaacaagca gtatacagaa tcccagcttc gagcaggagc tggctatatc
     4201 cttgaagatt tcaatgaagc ccagtgtaac acagcttacc agtgtcttct aattgcggat
     4261 cagcattgtc gaacccggaa gtacttcctg tgccttgcca gtgggattcc ttgtgtgtct
     4321 catgtctggg tccatgatag ttgccatgcc aaccagctcc agaactaccg taattatctg
     4381 ttgccagctg ggtacagcct tgaggagcaa agaattctgg actggcaacc ccgtgaaaat
     4441 cctttccaga atctgaaggt actcttggta tcagaccaac agcagaactt cctggagctc
     4501 tggtctgaga tcctcatgac tggtggtgca gcctctgtga agcagcacca ttcaagtgcc
     4561 cataacaaag atattgcttt aggggtattt gatgtggtgg tgacggaccc ctcatgccca
     4621 gcctcggtgc tgaagtgtgc tgaagcattg cagctgcctg tggtgtcaca agagtgggtg
     4681 atccagtgcc tcattgttgg ggagagaatt ggattcaagc agcatccaaa atataaacac
     4741 gattatgttt ctcactaagg taccgcggcc gcggggatcc agacatgata agatacattg
     4801 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4861 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4921 attgcattca ttttatgttt caggttcagg ggg
//
