LOCUS       sequence-46616-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY013
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(553..573)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(580..645)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(652..705)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ccaattaaaa gctcttctac actaagaaca gctccattac aattagtgat atgatttaat
       61 cgtgtaagct gagctttgta gtttccttta aagtgaccac tcacaaataa aaacttaaat
      121 tccgttacag aagatggata gactttccac cattcattag ggttgagatg tttgtttcgt
      181 gtttgatttt cttcgacata tcgctccatt tcatctgctt ggccaattgg cagattataa
      241 cctccgctat aagctttagt atccacgatc acaccgtaat caataggaga tccgacagta
      301 taaattgctc cgtccggttt ccttgatcca cccaaatgtt tacctctata tccataaact
      361 ttcataaaaa attccattac cttcatttca agaattctat cctgagtgga atttctggca
      421 atttcaatta attcaatata ttcatgaggc acatatttca atttatgacg aagttcagat
      481 ttcttctcct ccagttcact tttgactagt tgagatctaa gcttactagc tagccggcac
      541 cccgtgaatg cccaccttcc tcttcttctt gggggccatc ttgtcatcgt catccttgta
      601 atcgatgtca tgatctttat aatcaccgtc atggtctttg tagtccatcc tagggccggg
      661 attctcctcc acgtcaccgc atgttagaag acttcctctg ccctctccgc cgccggacct
      721 gaaattgatt tcaccattgt tgaacttgcg gcgcacctct tccagggtca gggtaccagc
      781 cttgatcatc tcgccgccga tcagcagttc ctccacggac agcacggcgc cgttgcagtt
      841 gg
//
