LOCUS       sequence-46638-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-EZH2_2
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           138..152
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           180..306
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(400..419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(401..419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(473..496)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
       61 taggcgccgg aattagatct ctcgagaagg tatattgctg ttgacagtga gcgcagaggg
      121 aaagtgtatg ataaatagtg aagccacaga tgtatttatc atacactttc cctctttgcc
      181 tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
      241 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
      301 cacttttttc aattggaaga ctaatgcggc cggccagatt gtgatttttg tggggggctg
      361 gtatatttcg gtccgggctt atccggtctt tcttttgggc cctatagtga gtcgtattac
      421 ctaggacgcg ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      481 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      541 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      601 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      661 acaaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagc
//
