LOCUS       sequence-466515-       15145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suppressor screen mutant in full-length Prp8 on a TRP plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15145)
  TITLE     Hoskins lab plasmids 2025
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS424 Prp8 G1868R
FEATURES             Location/Qualifiers
     source          1..15145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(6447..6464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7263..7282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7264..7282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7292..7309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7292..7308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7301..7323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7449..7904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7536..7555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7746..7767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8007..8681)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(8682..8963)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(8984..9003)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9103..9125
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9163..9181)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      9222..10564
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9554..9601
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        10591..10695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10696..10764,10765..11556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10914..10933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11727..12315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12216..12235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12469..12486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12603..12624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12639..12644,12645..12662,12663..12669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12677..12693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12682..12704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12701..12717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12701..12717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12736..12756
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12738..12756
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atcagccgaa acataaatgt tttttaacct caaatataat agtgtattag caattgcaga
       61 ttttctccat tcattctttg aactaaacgt ttgactttcg taatcagcgg aagctactac
      121 aactatctcc tcaccttgcg cattgatagt tttagttttc ataacagttg atgcacctgc
      181 agcttcttcg tcgttctgct tttctgacct ggcagcttct aattcagcca ttttttgtct
      241 cttcacagat ggtgccttga tattttgacc caatataata tctttaattt ccgtttgagt
      301 taaggcagat atattgacat tgtattttct gccgtactca gtcaaaatca aatctctcat
      361 ttgagattcg atagtgatcc attgttcatc agtgaatgaa ggccataaat ggtacgattt
      421 gatagtgatc gtggggtcac tgagtagaat cattttggca ctttcctcgt tcgtctttaa
      481 agctcttaaa agtagtgtaa gtctagaaaa tgcagtgtac gatgaaatac ggtctaacca
      541 atcatcataa atgttaaaca gaaccatctg aggttcagta gctttcatca caacatcaga
      601 aagtttatct attgacattg cagctgaaaa tggtagtcgc agctctgttg gcctgattgc
      661 tatgttagga aagtcaagca tgtgaacctc taaaggatca agcattgcct ttcttgtgac
      721 gataatttgt tttggctgct cttctttggg caaagatctc acaagagcac taacttcctc
      781 agctgttttc catttggcca attggcttaa acgtttttga ccggcccata cagaggtatg
      841 gataattttc agaaacagat gcccagtctt tggatttaaa gtgaaaatgc aaccattaat
      901 tgcttttgta gcaacgttcc tttcaaaagt cttgtggaca gtaactctat acacattagt
      961 gtcgtcaacg aagagtttaa tgtcgttatt gaataactcg gcatagtttg aggagtttaa
     1021 aaatggttcc tgtacactag attgatatat ttgaagacct tttctaatac gttcacgaag
     1081 gacatataat gctgggttag ctttcataat tgttctcata ctattttgta tcaaaggttt
     1141 taatccgttg aaccaattac cataggcatc atacatgtta taagccaaat cgataccgat
     1201 cataacacct gttggggagg gatacatgct gacattatct gttgtataat cgaggaattt
     1261 tgccctcaca taacgagata tatcatgaga atcataatca ccgtatctca attgtacatc
     1321 aaaccacatt ttgttggtaa tcaatccctt aaatgagtca tttgtttcat gtaataggga
     1381 gggttttgaa acttcccatt catgaacact ttccatagtt atgtcagcgg cagatgaatt
     1441 catcttgtac gatttgcggg gatgcactgt ttctttagtc acagattcaa tttgcaaaac
     1501 atctagctca ccatcgagaa tttgacaaat atcaaaaact atactttcat gtattttttg
     1561 ccacaaatga gcacggaata tttgaattaa agatattttt aacgtaggaa ttttaccatg
     1621 gaggaagata ccagtcagat ccaattgcac caagaaaccg acatagacat ttgctctgtt
     1681 aatagttggt gaccaccata aggtaaatct acgatttgga atttggctta aaccagttct
     1741 ttgagcatga gttagcttct taaattgcat ggaatcctca aacccggacg ccttttccca
     1801 aaacaaacct tcccatgaat taaaaccagt ccccttaaat aaagtgtgtt ccaaaattgt
     1861 ttcaatacct cctagggctt gaatcacatc agttctatag gcatttaaat tccataattt
     1921 accgtcatgg tgggagtttg tccaccaaaa ggggctgttt ctctctagag agtactgttt
     1981 aaattcccta cggattctgt gacctctgtc atacgctaga gtatgtcgat ctctttgaaa
     2041 taaagtgcta atacgaggta taccacgatc ccacgatcct tcaagttctt caaaagctaa
     2101 cctcctattc tgctgaatag cttcctgcct tttcgttgca tactcggccc acaccctttg
     2161 agagtctaaa aattcatttt cccaagtagt gatgtaacgg aagatagttg ggattaattt
     2221 ttcatcctcg tgcgtcatac cagcacgaaa atgcgtaatt ccagtatcgg tctgttttga
     2281 ccaacttaag tcagatgcag gaattaaaat atgagaagca ctaatcatac ccaacccacc
     2341 aagttctttt ggtgtataaa aaacagcagg tggaaatctt gttggcattt tcgaatttaa
     2401 accaagtttg acacggtttt gaattcttgt ttctcctttc accaggatat ctaataacgg
     2461 ctccgtggca acaatggctt ctctaaagta tgtgaaaagg gatatcagtg aagtgttcca
     2521 tttagcggcc actttagtaa aagtggttga accggaggcc atcaatatgc ctcttattct
     2581 gctgtcaaat tttttgattt cttcttcaga tacctttaaa tatgcttttg cagttctttg
     2641 cttggttctt tcgtcaacta aatcccaaac accttcatcg ttagaaacca cctcttccat
     2701 tctttgtctt ggtaatattc tgacttcaaa accacacata gagaagagta agttcggatt
     2761 gtttttactg tatacggaaa caaaagcatt ttcccattta attgacgtta acgaagttgg
     2821 caccctactt tgaatttccc aaaacactgc tcgccctagg ttaacgtcct gacgtattag
     2881 cctcatgcgc gaatctttgg gccagcattt tctattattg tatccgatgc tgttttcaaa
     2941 gtttggatct ggattctctg ccaagtattc atcagtcaat tcctctccct catcttcttc
     3001 aaagtgaaaa agcatatata tacgatctaa atatctggtg taaagtctga tcggatgtgc
     3061 cttttctact tctttgctct tgaattgcat aaattcattt ggattgttag ctggaccagc
     3121 caaatctgta gccctttcct gacccaatag taaaagatct ataactagtc cgtaatattg
     3181 gaatatgaaa gaagcgaact tcaacccgcg tattaagcca tatttgttga cgtgactcat
     3241 atctttaaag ttgataacaa cattattttt tgcagtgata tagtcagcaa tattaggatc
     3301 tacaatcagg cgaagtaatc tattaagtaa agtaaagtca attttttcgg ccatttcacc
     3361 caaagtagtc tcaagcaaaa ctgcagattg gcctctggat acatcccaaa tttcagatag
     3421 gttgtttata ccctgagtcc acttgtatac cagaagaggt ggtatctctg aatcacttgg
     3481 tttaatccag tttgggaaaa gctttcgttg gtcagcttcg taccataaat actgatcaag
     3541 atatgcatcg gtaatctttt ccaagggatc aactgaataa acaggggaaa tgttttgata
     3601 gttttccatc atggttatat caacaggctt aaaaactcgc tgggtcaaca agtacttttt
     3661 gattctgttc aaagtatcgt gaggattatc ataagcctcc tctatcaacg caagttcttc
     3721 cctttcagat gcatttaaac gtacttttga tgcgtagaca tcttttaggt cttcgagggc
     3781 aagaactaat atcttagtat catttttgta agttaaaggt ggaaatggta ttggtgaaaa
     3841 gctccttgat tcaagccact caaccataac agaaaatatg gtcgtagcct cttcgggagt
     3901 aatttcggga ccgtttttct gtatttgcct ttgacgttcc tgctcatttt tgatccaaag
     3961 tctcgttaaa cgccccagat tttttttaac catagtcttt tcgacgtgag ctccacgctt
     4021 tatacgttct cgattgtaat gagctgcaga tacccaggca tcagctttgg attttatata
     4081 ccgttcaatt atttttttga ttggggctgg cataccagga acatcccatg gaatattagc
     4141 cttccaacat ctccaagcct cacttaaatg ttgcagtata gttcttgcct ttttctgtcg
     4201 aatactctca ggcatcattt ctaaaatgtc atccatgacc gagtttcgta actcaagatc
     4261 gtagtaggcg tcaagtcttt gtttagttgt agttttgaca atttcattag aacggccttc
     4321 aaattgacgt gtgataaggt ttccaatata tctttcgagt agtggtatag tgcctcttaa
     4381 gaaatttagc cacactctcc acgcaggttg ccaaaatcca caaccaggtc cttttcccaa
     4441 atttttatta aacttataat atatgatatg tttcagatct ttacatgcgc ggatttgatg
     4501 cattacttta tatttataac gataaatacc ggtcaattgt cctatgtggt tcaaaatata
     4561 atgtatacca tctgctaact ggaaggcatc tacatttcct aacctaaact gcacatgtgt
     4621 atctacaatc agtttcatca tttttagcaa ttcacgcatc aaatgaaatg agtttccaag
     4681 tcttgacttt ttacgttctt ttgtggttaa ggttttagtt ggttttaaat tgaaattgta
     4741 gtcgagatgt aaataagtta ggccctttct gtggattaat aaatttaaca tattgtgtcc
     4801 ttgtcgacat agctggagtc ctgcctctac ccaatcaatt gtagtttgtt gaaaatactt
     4861 ggtatttttt aaactcttca ataacttagt tttgttgtgg tttgttggca aagtagggtg
     4921 caattcattt agcacgtagt tcttcaagag cttttggtaa gagaccttaa ctttaacggg
     4981 atactcctcg tcaggatgtt gcaaaaacca ttttttagcc agggcaacat cttgtgctct
     5041 caccattttt cctttagttc tattaaatgg aaaaggtgaa tgatataacg acatcgcatc
     5101 ttttgtattt ggcaaaataa gttcttcttc ttcagccaaa agaggtgcaa aatcttcagg
     5161 taaagtgaaa tcaccgtttt cctttgtatt tgatacgttg agggatgaat tattatcaat
     5221 aaagtgaggt attgggttca gcgatggatc aaaaaaaagt gcaggcgtgt cgtactcctc
     5281 atcgttctgg ataatacacg acactggatt attataccat ggtatgcgca ctgaacgggg
     5341 ccgggaatta tatagatgag gaaacgctac cttatattcg cttctaatag gaaccctaaa
     5401 tattactcta tctatagagt tgaattcgtt ataatcttcc tcctcttctc ttgggtataa
     5461 aggttcaaat ttaggacctc cagggattgc gttattaagg gctttaccgt taaaaaaaga
     5521 ttttttatcg aaaagataat agtaattttt gtcagttact tcatctctaa gtggcgttga
     5581 taacctatat aagttcgaca tttcaggtaa atcaaaactc cactttttat aagatgtacc
     5641 attgaccttt ttactatcct cctccaaggg ccgggaatcg tagagccaat ctttaacata
     5701 ttcatcatct tgagagtcta agggaagatt tataggatcc agtggctcga tattttctat
     5761 gtgctgttca tatgacagag gtggttcgtc atcatcgaaa ggtggaaatc tcattctttt
     5821 aaaatgtgtt ctatctcgtt tttctctcct cattgctatc caagtagctg accattgggc
     5881 agtatataca ggctctatta ctcttggtgt ttcattcaca aatgttatgg ctcctgaagt
     5941 gtgatataaa actttaactt ctttggcttg ctcccatgga tgaggcatgt tctctaacaa
     6001 ttttaaaatc gcgtgcggta aatactttag agcgcctaga aaagcctttt tatcagtatt
     6061 gtacattttg gaggccatgt cactgtgagt attaataatc tttcgcagat gttccggtgg
     6121 catctcggct ttaggagtat ataaattgct tctctttgcc tttttggtca ttttttttgc
     6181 acgctttctt gattttcgag tgacgaaagt atcttttcct atgtccaatt ttctctttcc
     6241 atgtaattct actttctttt ctgctttagt ctctaattca tctagacctg gagggggtgg
     6301 tggcggtggt aatgtgaagt ccacaatttc ttcagcgttt atttcgaagt tgcttggagg
     6361 aggtggtgga ggcggaagga atgtatcctc atttaccgat gatggcacca tcggattatc
     6421 cagttcttcg atttcgtatc caggcggtgg tggtggtggt ggtggaagtg ctaagtcgct
     6481 gtcctcttca aaaccaggag gtgggggcgg tagtccactc atctttcttt gacgctattt
     6541 tttttttgga aggaagttca atcgcacaag tattttctat ggtcttcgtt tctcaaattg
     6601 tattcctaac tttgttgctt ttgtactagc cgtattacct cggttcatcc ttctgccatg
     6661 gtcactttaa atagctatat agcctggcat ttataaaaat ttcatatcat ttagtacgaa
     6721 tagctatgtg tatgttcact tcactacata tgccttctgc actcccgagc cagcatacga
     6781 gcctcttcaa tctcttgaga cgttccacta ggtaccccca tggcataatc ataagccaat
     6841 tggtagtttc ctgccttaat ctcaaatatt gccagccaaa gcctagcctt cacggtttca
     6901 tctgttacta taccttccag aagttcttcc acatctacac atttcatcat gaacttctta
     6961 cattcttgca agtcttcaag ttcttcatat agttgcgcta accggtaata tattgaagta
     7021 ttttgatcga ccgtttgtga agcttttatg gatcttttgt agcattttat agcttctacc
     7081 ttatttcccg tcttactata acattctccc aatacttgcc aaatccgacg atcccaaggt
     7141 ttcaaagtgc aagctttctg gaagtagtaa agagaatata aatgcatgtc caggagagca
     7201 taagcctggc ccaaaccaaa ccatgctttg aagtctcgag ggggggcccg gtacccaatt
     7261 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7321 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7381 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7441 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7501 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7561 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7621 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7681 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7741 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7801 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7861 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     7921 ctccttacgc atctgtgcgg tatttcacac cgcatagatc ggcaagtgca caaacaatac
     7981 ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt
     8041 ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg
     8101 ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa
     8161 tgtaagcttt cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa
     8221 agttcacctg tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt
     8281 gaagctgcac tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc
     8341 aaaccgagga actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca
     8401 atgccgtaat cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca
     8461 accaagtatt tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc
     8521 cttgcaataa ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag
     8581 tcagcatcgg aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc
     8641 accaatggac cagaactacc tgtgaaatta ataacagaca tactccaagc tgcctttgtg
     8701 tgcttaatca cgtatactca cgtgctcaat agtcaccaat gccctccctc ttggccctct
     8761 ccttttcttt tttcgaccga attaattctt aatcggcaaa aaaagaaaag ctccggatca
     8821 agattgtacg taaggtgaca agctattttt caataaagaa tatcttccac tactgccatc
     8881 tggcgtcata actgcaaagt acacatatat tacgatgctg ttctattaaa tgcttcctat
     8941 attatatata tagtaatgtc gttgatctat ggtgcactct cagtacaatc tgctctgatg
     9001 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9061 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9121 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     9181 ttttataggt taatgtcatg ataataatgg tttcttagta tgatccaata tcaaaggaaa
     9241 tgatagcatt gaaggatgag actaatccaa ttgaggagtg gcagcatata gaacagctaa
     9301 agggtagtgc tgaaggaagc atacgatacc ccgcatggaa tgggataata tcacaggagg
     9361 tactagacta cctttcatcc tacataaata gacgcatata agtacgcatt taagcataaa
     9421 cacgcactat gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg
     9481 cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt
     9541 tcggaaacgc tttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     9601 cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc
     9661 accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa
     9721 agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt
     9781 cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt
     9841 actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg
     9901 aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa
     9961 ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat
    10021 ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct
    10081 tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata
    10141 gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag
    10201 actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa
    10261 tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa
    10321 aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
    10381 aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt
    10441 ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
    10501 tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt
    10561 cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    10621 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    10681 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    10741 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    10801 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10861 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10921 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    10981 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    11041 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    11101 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    11161 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    11221 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    11281 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    11341 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    11401 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    11461 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    11521 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    11581 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    11641 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    11761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    11881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    12001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    12061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    12121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    12181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    12241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    12301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    12361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    12421 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    12481 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    12541 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    12601 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    12661 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    12721 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc
    12781 ggtggcggcc gctctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
    12841 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
    12901 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcttcaatt tctcctctcg
    12961 attttgtgtt atcattcttc ccacaacttt cctgggctcc acttttttga ttggtgttgc
    13021 cttgaattgt tatctgactc tcactcttgc gtttctcaac atacttttga gttaaaatat
    13081 ctatcaaaga gtcatcgcca tcgttgctat ctcctgttga atccatttta tctacttctt
    13141 ctgcattcac ctctgctttt ttttcaacgt tgggtgcctt gatgggtgaa gatggcaaat
    13201 cgctgaattt cgttatcgtc ggttttatag ttaaatcgcc ctctatatca tcgagaatat
    13261 catctatatt acggagagac tccctggtag atatttctgc tggtttagga cgcacttgct
    13321 catttccatt ttcttgaatg agttgattta cgtcagcgtt gggtacttgg atatttgtga
    13381 aactaagaac agttttcagt cgattaggga ctataggtgt agaagaggga ttttggacat
    13441 cttcgtagtc atcagagtat tcggcagcgc ttgttgtttt gtcggacata tctcgttgta
    13501 aaggtgaata tttccatatt acttcttcca tggggccgtc ctcgtcttga gtgaaatcgt
    13561 gatgcctact caaattagac atttcccgtt tcggtttaac ttgtgaattt ggtaattgct
    13621 ttacgctctt gctaactgct ttgtttatat cttttgttct tgatgtattt cgtacttgtg
    13681 aaacggctat tgactttagg acacttgcat tggatacctt ggtgttcttc ccatttaagt
    13741 tatttatagg agcaaaagca tacttttttt tcctcttagt ttgcttagat aatatcgcct
    13801 ttgaatcatt ttgtattatt tctttttcct ctgtcttctt cgcaggtgaa acagatatac
    13861 tcgcagacct cttgttcttc tgtggcgttc cgggcatcct gacgatctct tcaattgtca
    13921 gtgtttgctt gcacaaaatg agtactcact tgagtatgtt ttctcccaat tttgttttga
    13981 ggctgccgta atgattgtaa ctttatcttc aaaaatgtaa cacaagtttt gccataaata
    14041 gcgtaagatc agttaaatat atataagttc tttctgccgc acggaattgc tttattgcgc
    14101 gaaatgaaaa caagaatatc caatttctat atgaaggaca taatggaatg ggagtgacaa
    14161 cggaccattt tctgtccgaa cgatgtctat ctcctttggt tactttttga aatagaatca
    14221 cagtcaatga ccaattttca catacttata gcttttcata gtgcggaaag cgtagctttg
    14281 aagtagttcc gaaattataa tacatcatcg tgtggtaggt agcaactacg acgggcataa
    14341 aaaatgcgtt gaatattata catacaaact tatacttaca tatatatcta tgaaataaca
    14401 gattccagtt tattggggaa tatattcagc taaatacatc gatttgttcg gcctctaact
    14461 cttcgtcccc cgccaattcg ctaaactgta aaaagtgtac tggacgatgc atctcattat
    14521 agaattccaa aggaatgccg tatttaaaat tgtagtcacc ttcttggtta aatgctgttc
    14581 ccataaatgt gtagttccaa acgtttccag atggaattat aaaattacct gtgatacggt
    14641 cagagagaag taattgggca tgcgtgctga aagtaggctc aaacccttcc gatagcacgt
    14701 tcattatatc cttattttct tcaccccatt ggtaaccttc gtcggtcagg ttataagcgc
    14761 ttaatgatac tgatcccggg gtggaaaaaa ttgaaatatc aatgcagtct cttttcttat
    14821 ccgcaaaaag tttagaatga gttgctactt cagacgcagc catgaattta agttcttctg
    14881 tctgggtatg gatccaaccg agtagttcaa ggccttcagt atccggaagg tctccgatgt
    14941 cagggatgtt gcttatctga acggatccta cgtggcctaa ttgaggtacc agcacaacag
    15001 tcttgatttc tttcaccttc ggatgatctt ttgctgacat accataaata aaggccgcca
    15061 cctggatttt cacatcggat atctcaataa acttctttaa taaatttttt ggaaggacgt
    15121 aaacattctg ttcctccacg aaatc
//
