LOCUS       sequence-466649-        9225 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged,
            optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal 
            sequence to help target construct to the ER .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(977..1384)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(1685..2140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1822..1843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2034..2053
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2266..2288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2280..2297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2281..2297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2307..2326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2307..2325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2344..2591)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2557..2575
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(4038..4067)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4068..4097)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     sig_peptide     complement(4836..4895)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     complement(5669..5689)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5669..5687)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5708..5724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5708..5724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5721..5743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5732..5748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5756..5762,5763..5780,5781..5786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5801..5822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5939..5956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6110..6698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6190..6209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6869..7660,7661..7729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7492..7511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7730..7834)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7871..8374
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8416..8434
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8472..8494)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8594..8613
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(9107..9225,1..976))
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca
       61 agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa ccgtggcatg
      121 gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca
      181 acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt
      241 tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag
      301 aatcaatcaa ttgatgttga accttcaatg tagggaattc gttcttgatg gtttcctcca
      361 cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag
      421 gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt
      481 ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct
      541 taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa
      601 attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta
      661 agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac
      721 taccggtacc ccatttagga ccacccacag cacctaacaa aacggcatca gccttcttgg
      781 aggcttccag cgcctcatct ggaagtggaa cacctgtagc atcgatagca gcaccaccaa
      841 ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa
      901 ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct
      961 tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat attgctgaaa
     1021 tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca
     1081 ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa
     1141 cgcttctcta ttctatatga aaagccggtt ccggcgctct cacctttcct ttttctccca
     1201 atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc
     1261 agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa
     1321 aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac
     1381 agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg gccaaacaac
     1441 caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt ttgaacacac
     1501 atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg atgtaattgt
     1561 tgggattcca tttttaataa ggcaataata ttaggtatgt agatatacta gaagttctcc
     1621 tcgaccggtc gatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     1681 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     1741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1921 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1981 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     2041 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     2101 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     2161 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     2221 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     2281 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
     2341 gccgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat
     2401 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt
     2461 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac
     2521 tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc gtgacataac
     2581 taattacatg actcgagtta agggcctgca gtattttctg aactatgttc gtttgagctt
     2641 gaattctctt cttttgaagc tggagagtag caaagacttt ccccttcaaa attggagctt
     2701 gcagaattat gaagcttttc tgaataatcg agagtaactt ttgagaatcc tccaagtgat
     2761 atttcgtcgt caaagctctg tccttgcgtg acgtaaggga tgtgcctact ttcttttagg
     2821 tctatttcat caagaatatc atctgtttct ttatcagcaa acaaatattt tttgctttta
     2881 ttttgagatt ttctaggatc atctggaatc ctatagtcga catagaaatg cgcttgtgat
     2941 attggtgtac ctacacaggg agaatagttt tctgtacacg tggagatata gttctcagag
     3001 tctgtagaaa atgggttacg gctctctttg cgtgaggaca gcttgtttag caatatgcct
     3061 actcgttttt ccttattctt atcaatgaat cttttccaca tgtcaagtac aaatcctagt
     3121 ttgatggaac gcaggaattt agagtattaa tgcaaagcat cagaacctaa gccaaagatt
     3181 agaaatacta ggtaagacat caaaacataa agccatatat tataaattgg tctgccaggg
     3241 tcaaatttaa taatggtatt ccagatggtg ctggaatggg tatttttaaa agtatagtgt
     3301 ccttctacct gctgtaaatc ttgaacaaag gtgtaaacag aaaaagggaa catgactaaa
     3361 ataataatga aacagaatat caacagcctt gcgaaccttg tcaggtttaa acctgaattg
     3421 gtacagtgta aaatatccct aacgtccttg cgttttttat aaaacacgaa cagtactaag
     3481 gtggcataaa cagcgcccac aaatgaccat ataagcatcc acatggtata caaaacagtg
     3541 gtaatccatg tcggagacaa taagttttgg caaccgttgt aacgagcgat accatatcta
     3601 aatacttgta acagatatga aaatcccatg accatgacag gtgtgaacaa gctaatcaca
     3661 aggtccttga cgattttcgt ccatgatgaa agatccggta aaacactatc tgccttcaaa
     3721 attgtatgca agttgtaaat gatgttggta acggcacatg atatgccaat attcgcacca
     3781 acctgcaact tgatgacaat atcacaccaa cctttaccat cccatctcgt gaggaaatcg
     3841 tcgtcactcc atattgccgc atctacaata cacgttaaat tcattgtaag aagccatgtt
     3901 atcaaaatga ttgctggaat attcttggta tgtgaatgcc atgctaaagg gggagctaat
     3961 agtatcacag ctagcaaaca aagccctatt attgctgact tgtatgacat cccggggcga
     4021 cccgcacccg cacccgccag gtcctcctcg ctgatcagct tctgctccag gtcctcctcg
     4081 ctgatcagct tctgctcaga caaaacagtc aattgagtac caccaccgaa caagtaaccg
     4141 tcaacgtcag acaaagaaca gtagtattca gcttcgtctt ctggttgagc gtcagagata
     4201 gtcaaagcag ccttagcacc caacaaagaa ccagagaatc tacctggagt ccaagagtac
     4261 ttcttgtcag tgtcgaagat caaagctctt ggagcttgac ctggcttttg ttggaaccag
     4321 ttagcgtagt gaccagaagt aacagcacca gtaccagaac cacaagtcaa gataacagta
     4381 ccacctggag aaacagtaac agatggttct tgagtaacaa cagcttgaga accaccacca
     4441 ccagaaccac caccaccaga accaccacca ccagaaccac caccaccaga caaaacagtc
     4501 aattgagtac caccaccgaa caagtaaccg tcaacgtcag acaaagaaca gtagtattca
     4561 gcttcgtctt ctggttgagc gtcagagata gtcaaagcag ccttagcacc caacaaagaa
     4621 ccagagaatc tacctggagt ccaagagtac ttcttgtcag tgtcgaagat caaagctctt
     4681 ggagcttgac ctggcttttg ttggaaccag ttagcgtagt gaccagaagt aacagcacca
     4741 gtaccagaac cacaagtcaa gataacagta ccacctggag aaacagtaac agatggttct
     4801 tgagtaacaa cagcttgcat ggatccacta gtgtcaccag tggaacctgg aacccagagc
     4861 agcagtaccc atagcaggag tgtgtctgtc tccatatcca aagatacccc ttcttcttta
     4921 gcagcaatgc tggcaatagt agtatttata aacaataacc cgttatttgt gctgttggaa
     4981 aatggcaaaa cagcaacatc gaaatcccct tctaaatcta agtaaccgat gacagcttca
     5041 gccggaattt gtgccgtttc atcttctgtt gtagtgttga ctggagcagc taatgcggag
     5101 gatgctgcga ataaaactgc agtaaaaatt gaaggaaatc tcattctaga gaaaattttg
     5161 atagtatttt gcctttccta cacaaattta attgcctcta aagttaaaaa gcaataaaat
     5221 agaatatgtg aatactgcac aaaatgaata cttgggaatt cagaaattat tcttacctta
     5281 agcatccact catatacata tatatatata tatatgtctt ttcaaaagac ttctgcccaa
     5341 attgtggaga gaactctgcc tctccgtgat aacggccaaa tgaaacgcat ccaatgatgc
     5401 aattattgac aaagtttcaa agtttccgcc attattcctt catttacaca ttgatagtcg
     5461 agaagtttcc taattagtgt cacaatgaca gagaaaattg taccagcgct tctaacgaaa
     5521 attagcaccc atacccagag ttcataaatc tggcaatgtg gtacaataat ataggattgg
     5581 taacatttca attgtaaaat gtttaacgtt tagagaaaag gggagccaga acgcttctgg
     5641 tggtgtaaat gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5701 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5761 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5821 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7861 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
     7921 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
     7981 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
     8041 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
     8101 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
     8161 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
     8221 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
     8281 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
     8341 atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg
     8401 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8461 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8521 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8581 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat cgactacgtc
     8641 gttaaggccg tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg
     8701 gttcaagaag gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt
     8761 tgttgtattt tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt
     8821 catgattttc tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg
     8881 ttaaagtgca attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt
     8941 attcctttac atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt
     9001 ttcgtctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt
     9061 catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt
     9121 tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat
     9181 caccagttct gatacctgca tccaaaacct ttttaactgc atctt
//
