LOCUS       sequence-46665-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Ahn D, Ho RK
  TITLE     Tri-phasic expression of posterior Hox genes during development of 
            pectoral fins in zebrafish: implications for the evolution of 
            vertebrate paired appendages.
  JOURNAL   Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3.
  PUBMED    18638469
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2008-10-1"; volume: "322(1)"; pages: "220-33. Epub 2008 Jul 
            3"
COMMENT     Sequence Label: Hox A13a
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     77..93
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     77..93
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(771..789)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(772..789)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(807..823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(820..842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    831..847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(855..861,862..879,880..884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggcggccgcg ggaattcgat taattaaccc tcactaaagg gaacaaaagc tggagctcca
       61 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcagggat tcggcacgag
      121 atactatcct tgccgaacgt cccaccacca tgggagtggt ggaggtgtaa agacatgcgc
      181 ccagtcccct gcctctggtt caccttacgg agaaaaatac atggacacat cagcttctac
      241 aggcgaagat tataccagct cacgcgccaa ggagtttgcc ttgtactcaa gctacgcctc
      301 aagtccatat caaccagttc cgagctatct ggatgttcca gtagtccagg caataagcgg
      361 gccttcggag ccccgacatg agtctttact gccgatggag agttaccaac cgtgggcaat
      421 cacaaccagt ggatggaacg gacaggtcta ctgcaccaaa gagcagcaac aaacgggaaa
      481 cgtgtggaag tcgtcaatac cagagtcagt atcccatgga ggggcagacg gcagctcctt
      541 ccgccgcgga aggaagaaac gtgttccata taccaaggtg cagctgaagg agcttgaacg
      601 ggagtatgcc actaacaagt ttatcaccaa ggacaaacgc agacggatat ccgctcagac
      661 caacctatct gaacgacagg tcactatctg aatcactagt gaattcgcgg ccgcctgcag
      721 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
      781 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaa
//
