LOCUS       sequence-466691-        9225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAP tagged STE3 (N-terminally tagged, optimized for yeast 
            expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal 
            sequence to help target construct to the ER .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(773..1180)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(1481..1936)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1618..1639)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1830..1849
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2062..2084
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2076..2093
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2077..2093
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2103..2122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2103..2121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2140..2387)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2353..2371
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3834..3863)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3864..3893)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     sig_peptide     complement(4632..4691)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     complement(5465..5485)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5465..5483)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5504..5520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5504..5520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5517..5539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5528..5544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5552..5558,5559..5576,5577..5582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5597..5618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5735..5752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5906..6494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5986..6005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6665..7456,7457..7525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7288..7307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7526..7630)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7667..8170
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8212..8230
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8268..8290)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8390..8409
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(8903..9225,1..772))
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 cggaggcttc atcggagatg atatcaccaa acatgttgct ggtgattata ataccattta
       61 ggtgggttgg gttcttaact aggatcatgg cggcagaatc aatcaattga tgttgaacct
      121 tcaatgtagg gaattcgttc ttgatggttt cctccacagt ttttctccat aatcttgaag
      181 aggccaaaac attagcttta tccaaggacc aaataggcaa tggtggctca tgttgtaggg
      241 ccatgaaagc ggccattctt gtgattcttt gcacttctgg aacggtgtat tgttcactat
      301 cccaagcgac accatcacca tcgtcttcct ttctcttacc aaagtaaata cctcccacta
      361 attctctgac aacaacgaag tcagtacctt tagcaaattg tggcttgatt ggagataagt
      421 ctaaaagaga gtcggatgca aagttacatg gtcttaagtt ggcgtacaat tgaagttctt
      481 tacggatttt tagtaaacct tgttcaggtc taacactacc ggtaccccat ttaggaccac
      541 ccacagcacc taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa
      601 gtggaacacc tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact
      661 tgacattgga acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt
      721 cttgaccaac gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg
      781 tatatccttg aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
      841 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
      901 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
      961 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat
     1021 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg
     1081 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc
     1141 gaactcttgc atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc
     1201 ttgaatcagg cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt
     1261 ataattatcc tataaatata acgtttttga acacacatga acaaggaagt acaggacaat
     1321 tgattttgaa gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca
     1381 ataatattag gtatgtagat atactagaag ttctcctcga ccggtcgata tgcggtgtga
     1441 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     1501 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1561 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1621 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1681 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1741 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1801 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1861 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1921 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1981 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     2041 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     2101 cgtaatacga ctcactatag ggcgaattgg gtaccggccg caaattaaag ccttcgagcg
     2161 tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta
     2221 cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa
     2281 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
     2341 tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc gagttaaggg
     2401 cctgcagtat tttctgaact atgttcgttt gagcttgaat tctcttcttt tgaagctgga
     2461 gagtagcaaa gactttcccc ttcaaaattg gagcttgcag aattatgaag cttttctgaa
     2521 taatcgagag taacttttga gaatcctcca agtgatattt cgtcgtcaaa gctctgtcct
     2581 tgcgtgacgt aagggatgtg cctactttct tttaggtcta tttcatcaag aatatcatct
     2641 gtttctttat cagcaaacaa atattttttg cttttatttt gagattttct aggatcatct
     2701 ggaatcctat agtcgacata gaaatgcgct tgtgatattg gtgtacctac acagggagaa
     2761 tagttttctg tacacgtgga gatatagttc tcagagtctg tagaaaatgg gttacggctc
     2821 tctttgcgtg aggacagctt gtttagcaat atgcctactc gtttttcctt attcttatca
     2881 atgaatcttt tccacatgtc aagtacaaat cctagtttga tggaacgcag gaatttagag
     2941 tacatatgca aagcatcaga acctaagcca aagattagaa atactaggta agacatcaaa
     3001 acataaagcc atatattata aattggtctg ccagggtcaa atttaataat ggtattccag
     3061 atggtgctgg aatgggtatt tttaaaagta tagtgtcctt ctacctgctg taaatcttga
     3121 acaaaggtgt aaacagaaaa agggaacatg actaaaataa taatgaaaca gaatatcaac
     3181 agccttgcga accttgtcag gtttaaacct gaattggtac agtgtaaaat atccctaacg
     3241 tccttgcgtt ttttataaaa cacgaacagt actaaggtgg cataaacagc gcccacaaat
     3301 gaccatataa gcatccacat ggtatacaaa acagtggtaa tccatgtcgg agacaataag
     3361 ttttggcaac cgttgtaacg agcgatacca tatctaaata cttgtaacag atatgaaaat
     3421 cccatgacca tgacaggtgt gaacaagcta atcacaaggt ccttgacgat tttcgtccat
     3481 gatgaaagat ccggtaaaac actatctgcc ttcaaaattg tatgcaagtt gtaaatgatg
     3541 ttggtaacgg cacatgatat gccaatattc gcaccaacct gcaacttgat gacaatatca
     3601 caccaacctt taccatccca tctcgtgagg aaatcgtcgt cactccatat tgccgcatct
     3661 acaatacacg ttaaattcat tgtaagaagc catgttatca aaatgattgc tggaatattc
     3721 ttggtatgtg aatgccatgc taaaggggga gctaatagta tcacagctag caaacaaagc
     3781 cctattattg ctgacttgta tgacatcccg gggcgacccg cacccgcacc cgccaggtcc
     3841 tcctcgctga tcagcttctg ctccaggtcc tcctcgctga tcagcttctg ctcagacaaa
     3901 acagtcaatt gagtaccacc accgaacaag taaccgtcaa cgtcagacaa agaacagtag
     3961 tattcagctt cgtcttctgg ttgagcgtca gagatagtca aagcagcctt agcacccaac
     4021 aaagaaccag agaatctacc tggagtccaa gagtacttct tgtcagtgtc gaagatcaaa
     4081 gctcttggag cttgacctgg cttttgttgg aaccagttag cgtagtgacc agaagtaaca
     4141 gcaccagtac cagaaccaca agtcaagata acagtaccac ctggagaaac agtaacagat
     4201 ggttcttgag taacaacagc ttgagaacca ccaccaccag aaccaccacc accagaacca
     4261 ccaccaccag aaccaccacc accagacaaa acagtcaatt gagtaccacc accgaacaag
     4321 taaccgtcaa cgtcagacaa agaacagtag tattcagctt cgtcttctgg ttgagcgtca
     4381 gagatagtca aagcagcctt agcacccaac aaagaaccag agaatctacc tggagtccaa
     4441 gagtacttct tgtcagtgtc gaagatcaaa gctcttggag cttgacctgg cttttgttgg
     4501 aaccagttag cgtagtgacc agaagtaaca gcaccagtac cagaaccaca agtcaagata
     4561 acagtaccac ctggagaaac agtaacagat ggttcttgag taacaacagc ttgcatggat
     4621 ccactagtgt caccagtgga acctggaacc cagagcagca gtacccatag caggagtgtg
     4681 tctgtctcca tatccaaaga taccccttct tctttagcag caatgctggc aatagtagta
     4741 tttataaaca ataacccgtt atttgtgctg ttggaaaatg gcaaaacagc aacatcgaaa
     4801 tccccttcta aatctaagta accgatgaca gcttcagccg gaatttgtgc cgtttcatct
     4861 tctgttgtag tgttgactgg agcagctaat gcggaggatg ctgcgaataa aactgcagta
     4921 aaaattgaag gaaatctcat tctagagaaa attttgatag tattttgcct ttcctacaca
     4981 aatttaattg cctctaaagt taaaaagcaa taaaatagaa tatgtgaata ctgcacaaaa
     5041 tgaatacttg ggaattcaga aattattctt accttaagca tccactcata tacatatata
     5101 tatatatata tgtcttttca aaagacttct gcccaaattg tggagagaac tctgcctctc
     5161 cgtgataacg gccaaatgaa acgcatccaa tgatgcaatt attgacaaag tttcaaagtt
     5221 tccgccatta ttccttcatt tacacattga tagtcgagaa gtttcctaat tagtgtcaca
     5281 atgacagaga aaattgtacc agcgcttcta acgaaaatta gcacccatac ccagagttca
     5341 taaatctggc aatgtggtac aataatatag gattggtaac atttcaattg taaaatgttt
     5401 aacgtttaga gaaaagggga gccagaacgc ttctggtggt gtaaatgagc tccagctttt
     5461 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     5521 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5581 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5641 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5701 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6961 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7261 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7621 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca cgtgctataa
     7681 aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa
     7741 aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc
     7801 gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt
     7861 ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat
     7921 cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa
     7981 gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac
     8041 tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac
     8101 acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag
     8161 gcaagcgatc cgtcctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8221 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8281 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8341 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     8401 cagattgtac tgagagtgca ccatatcgac tacgtcgtta aggccgtttc tgacagagta
     8461 aaattcttga gggaactttc accattatgg gaaatggttc aagaaggtat tgacttaaac
     8521 tccatcaaat ggtcaggtca ttgagtgttt tttatttgtt gtattttttt ttttttagag
     8581 aaaatcctcc aatatataaa ttaggaatca tagtttcatg attttctgtt acacctaact
     8641 ttttgtgtgg tgccctcctc cttgtcaata ttaatgttaa agtgcaattc tttttcctta
     8701 tcacgttgag ccattagtat caatttgctt acctgtattc ctttacatcc tcctttttct
     8761 ccttcttgat aaatgtatgt agattgcgta tatagtttcg tctaccctat gaacatattc
     8821 cattttgtaa tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat
     8881 atcataaaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat
     8941 caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca
     9001 aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt
     9061 tcaacatcat tgcagcagac aagatagtgg cgatagggtt gaccttattc tttggcaaat
     9121 ctggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag
     9181 aggccaagga cgcagatggc aacaaaccca aggaacctgg gataa
//
