LOCUS       sequence-466695-        6230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  39X FLAG tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6230)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-40XFLAG
FEATURES             Location/Qualifiers
     source          1..6230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1891..1914)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1927..1950)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1963..1986)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2017..2040)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2053..2076)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2089..2112)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2125..2148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2161..2184)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2215..2238)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2251..2274)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2287..2310)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2323..2346)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2359..2382)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2413..2436)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2449..2472)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2485..2508)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2521..2544)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2557..2580)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2611..2634)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2647..2670)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2683..2706)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2719..2742)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2755..2778)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2791..2814)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2845..2868)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2881..2904)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2917..2940)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2953..2976)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2989..3012)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3043..3066)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3079..3102)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3115..3138)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3151..3174)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3187..3210)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3241..3264)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3277..3300)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3313..3336)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3349..3372)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3385..3408)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3642..3661)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4650..4667)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4650..4666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4659..4681)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4875..4894)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4994..5016
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5140..5244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5245..6060
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5245..6060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5318..5337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgctcga tcgagccaag cttctacggc
     1021 tatctactta agtattattt ttagtaccta catcatgcct tgctcatgtt agttaaacac
     1081 aaaagcatac ggtgcattaa tatcaatgaa ttaaataata tcaacaaaca taaacagtaa
     1141 ttagttaaca gagtattcat atgacttttg attagctagt tctttttatg gttatcattc
     1201 gtgccgaaca tcaacaattg cttgggtttg attaaaatgt tctctttaca cagaagataa
     1261 tatcatcaac ggtaataaaa cttgcagttc gcagttcaac aacaatcttt ggctatatat
     1321 gtatatacga ttataccgat ataaatataa ttatcatagg ctttgtgtgg attatgtgat
     1381 ttatgatata cacaagtgtt gagtatatat ttccagttct ttccctatct tgtgttgcag
     1441 tttcgatttc ggagcttgta aatagaaatt cgcgtaagcg agaccctaat agtatatact
     1501 atatatgctt tatagatcga ttaggccagt ttgggtctat gtgggctaat ggtattggta
     1561 gtggttatcg gtattgctgt gtatgcatat gtagtttctc tgttaggtac atagtttcat
     1621 tgtgttagtg tggcatgtgg aatcccctta ttcgctgaca aattcgttac tctttagtta
     1681 tatatttata tacttctgaa aggtccacgg atccgtccat cgatggaact caacaaatag
     1741 agagaaaaag gggggaaaac tttaacggca caccgagaac aaacaataaa cgtcaaacga
     1801 tttccgacta caactaaaag ctatgatata tatcagtgac ctatcgcgaa cccattatgc
     1861 ggccgcggat ccgtcgacgc cgccggagcc cttatcgtcg tcatccttgt aatcgccgcc
     1921 ggagccctta tcgtcgtcat ccttgtaatc gccgccggag cccttatcgt cgtcatcctt
     1981 gtaatcgccg ccggagccct cgacgccgcc ggagccctta tcgtcgtcat ccttgtaatc
     2041 gccgccggag cccttatcgt cgtcatcctt gtaatcgccg ccggagccct tatcgtcgtc
     2101 atccttgtaa tcgccgccgg agcccttatc gtcgtcatcc ttgtaatcgc cgccggagcc
     2161 cttatcgtcg tcatccttgt aatcgccgcc ggagccctcg acgccgccgg agcccttatc
     2221 gtcgtcatcc ttgtaatcgc cgccggagcc cttatcgtcg tcatccttgt aatcgccgcc
     2281 ggagccctta tcgtcgtcat ccttgtaatc gccgccggag cccttatcgt cgtcatcctt
     2341 gtaatcgccg ccggagccct tatcgtcgtc atccttgtaa tcgccgccgg agccctcgac
     2401 gccgccggag cccttatcgt cgtcatcctt gtaatcgccg ccggagccct tatcgtcgtc
     2461 atccttgtaa tcgccgccgg agcccttatc gtcgtcatcc ttgtaatcgc cgccggagcc
     2521 cttatcgtcg tcatccttgt aatcgccgcc ggagccctta tcgtcgtcat ccttgtaatc
     2581 gccgccggag ccctcgacgc cgccggagcc cttatcgtcg tcatccttgt aatcgccgcc
     2641 ggagccctta tcgtcgtcat ccttgtaatc gccgccggag cccttatcgt cgtcatcctt
     2701 gtaatcgccg ccggagccct tatcgtcgtc atccttgtaa tcgccgccgg agcccttatc
     2761 gtcgtcatcc ttgtaatcgc cgccggagcc cttatcgtcg tcatccttgt aatcgccgcc
     2821 ggagccctcg acgccgccgg agcccttatc gtcgtcatcc ttgtaatcgc cgccggagcc
     2881 cttatcgtcg tcatccttgt aatcgccgcc ggagccctta tcgtcgtcat ccttgtaatc
     2941 gccgccggag cccttatcgt cgtcatcctt gtaatcgccg ccggagccct tatcgtcgtc
     3001 atccttgtaa tcgccgccgg agccctcgac gccgccggag cccttatcgt cgtcatcctt
     3061 gtaatcgccg ccggagccct tatcgtcgtc atccttgtaa tcgccgccgg agcccttatc
     3121 gtcgtcatcc ttgtaatcgc cgccggagcc cttatcgtcg tcatccttgt aatcgccgcc
     3181 ggagccctta tcgtcgtcat ccttgtaatc gccgccggag ccctcgacgc cgccggagcc
     3241 cttatcgtcg tcatccttgt aatcgccgcc ggagccctta tcgtcgtcat ccttgtaatc
     3301 gccgccggag cccttatcgt cgtcatcctt gtaatcgccg ccggagccct tatcgtcgtc
     3361 atccttgtaa tcgccgccgg agcccttatc gtcgtcatcc ttgtaatcgc cgccggagcc
     3421 ctcgagctta agggcgcgcc agccgccgga gccctgctgc tggtatccct gcggatcgta
     3481 ggacggcacc tgcaggaatg gattatttaa ataagtcaat taggatattg tcctatattc
     3541 caagagtttc attagggaat tagttattcc ttaatgaaac tcgatccaga catgataaga
     3601 tacattgatg agtttgggat ccagacatga taagatacat tgatgagttt ggacaaacca
     3661 caactagaat gcagtgaaaa aaatgctgcc cgggctaaac actgatttcg acggttaccc
     3721 cgcccaccaa tgacgctccc atcgagcgtt gaagtggcgc gacgctgacg ctcccatcga
     3781 gcgttgaagt ggcgcgacgc gagtttcatt agggaattag ttattcctta atgaaactct
     3841 gcacttagtc tcatgcagtt atagtatctt aagaaaatgt gctttgtgaa attaaaaata
     3901 tatatctaaa atatagttac ttcggaactc gaatagacaa acatcctttt tacaaagtat
     3961 cattctctcc cttccgattc gaagaaaaat gacatacttc ccagtccttg gcacaaagaa
     4021 atcgcattaa aataaatgct tttgttattt tttcctgccc tcccaggacc cccatccctt
     4081 tgagggctta ggacatgttt ccatttcaca tatttgcttt atcataaagc aaaatctaac
     4141 cctccccatt ttcgcaacat cacgcagccg taatccccgg ctttgatctg tggcaaaatc
     4201 gaattgggca agttgttagg ttggttgggc tacatacctc gaagagtcct tgcaggaccc
     4261 tcacgcaaat caccacatga ccgtgcatca gggtcatggc gaatggcacg aagagatgca
     4321 gcgtggcgga gctcacgatg gacagtccga atatcttgtt ggccgggaac ttggaggcga
     4381 tgaagccgcc gggaatctgc gtcaccagat agccccagaa gaaggacgag tccacatggc
     4441 tctccacggc gaccgtccag ttcatgaaca cagtctgcaa aaatccggaa acacatttta
     4501 cgggatgagt gacagaagct cagcaatcat taaaaactgc tccaggcatt tattaacgag
     4561 cgtcaagcag gattcagaat ttcaaattgg tttctatgat gggtctgtgt gggtgcgtgt
     4621 ccttttgtgt atgtatgact tttgaattca ctggccgtcg ttttacaacg tcgtgactgg
     4681 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4741 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4801 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
     4861 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4921 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4981 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5041 gcgagacgaa agggcctcgt gatacgctta tttttatagg ttaatgtcat gataataatg
     5101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5221 caataatatt gaaaaaggaa gagtatgagc catattcaac gggaaacgtc ttgctctagg
     5281 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     5341 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     5401 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     5461 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     5521 gatgcatggt tactcaccac tgcgatccct gggaaaacag cattccaggt attagaagaa
     5581 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     5641 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5701 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5761 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     5821 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     5881 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     5941 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     6001 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     6061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
