LOCUS       sequence-466711-        5540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chr18:43938484-43938985 cloned into pGL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5540)
  TITLE     Wysocka Lab enhancer plasmids 2025
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3_chr18q12.3
FEATURES             Location/Qualifiers
     source          1..5540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    568..659
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(600..619)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    673..721
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(852..1307)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(989..1010)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1201..1220
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1334..1438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1439..1507,1508..2299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1657..1676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2470..3058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2959..2978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3212..3229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3483..3604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3520..3539)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3574..3593
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3645..5297)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     5213..5231
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      5294..5303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5333..5529)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5347..5482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agagcctaga agaaggtgga gcatatgata aacactgacc aaaggttagc tgttttctca
       61 tcccacatat tatttaattt taagaacaac ccagaagttg tgtactatca ttatattata
      121 ggcgagaacg ttttggctta gagaggtgaa gcatgacttg cactaagtca tgtatctaat
      181 aaatggtgta gccaaattta gcatgaaagt ctgtgggaca ctacactttt tgttttctcc
      241 agggatgttt ctaattcaca ttctgaactt ggtaccacat gaacacttcc tttgatctaa
      301 gcagtccctt ccgttggaat cccctgagtt tacttcttct ctttggtgtt tcaagccact
      361 ggatttaatt aaagagaact gagtttctgt ttgttggaaa accttcagga aaggaaccta
      421 tttttaatga tttggaaaat gaagaattaa acaaatctga gcctgtagct gcaattatta
      481 gcaaatagtt tgggtgatag gactatggat ggagagaaat aaacgttcca cagcgctagc
      541 acgcgtaaga gctcggtacc tatcgataga gaaatgttct ggcacctgca cttgcactgg
      601 ggacagccta ttttgctagt ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta
      661 gtactatcga ttcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
      721 tgcggccgct ccaagtacct cccgtacctt aatattactt acttatcatg gtagcttggg
      781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      841 tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      901 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      961 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     1021 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     1081 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     1141 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     1201 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     1261 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     1321 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1381 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1441 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1501 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1561 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1621 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1681 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1741 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1801 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1861 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1921 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1981 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2041 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2101 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2161 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2221 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2281 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2341 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2401 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2461 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2521 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2581 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2641 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2701 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2761 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2821 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2881 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2941 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3001 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3061 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3241 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     3301 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     3361 tcggtcgacg gatccagatc cttatcgatt ttaccacatt tgtagaggtt ttacttgctt
     3421 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3481 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3541 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3601 cttatcatgt ctgctcgaag cggccggccg ccccgactct agaattacac ggcgatcttt
     3661 ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag ttttccggta
     3721 agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt cgcggttgtt
     3781 acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc gtgctccaaa
     3841 acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc gacacctgcg
     3901 tcgaagatgt tggggtgttg gagcaagatg gattccaatt cagcgggagc cacctgatag
     3961 cctttgtact taatcagaga cttcaggcgg tcaacgatga agaagtgttc gtcttcgtcc
     4021 cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc gttggtcgct
     4081 tccggattgt ttacataacc ggacataatc ataggacctc tcacacacag ttcgcctctt
     4141 tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc aaaaaatgga
     4201 acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag aatagctgat
     4261 gtagtctcag tgagcccata tccttgcctg atacctggca gatggaacct cttggcaacc
     4321 gcttccccga cttccttaga gaggggagcg ccaccagaag caatttcgtg taaattagat
     4381 aaatcgtatt tgtcaatcag agtgcttttg gcgaagaagg agaatagggt tggcaccagc
     4441 agcgcacttt gaatcttgta atcctgaagg ctcctcagaa acagctcttc ttcaaatcta
     4501 tacattaaga cgactcgaaa tccacatatc aaatatccga gtgtagtaaa cattccaaaa
     4561 ccgtgatgga atggaacaac acttaaaatc gcagtatccg gaatgatttg attgccaaaa
     4621 ataggatctc tggcatgcga gaatctcacg caggcagttc tatgaggcag agcgacacct
     4681 ttaggcagac cagtagatcc agaggagttc atgatcagtg caattgtctt gtccctatcg
     4741 aaggactctg gcacaaaatc gtattcatta aaaccgggag gtagatgaga tgtgacgaac
     4801 gtgtacatcg actgaaatcc ctggtaatcc gttttagaat ccatgataat aattttttgg
     4861 atgattggga gctttttttg cacgttcaaa attttttgca accccttttt ggaaacgaac
     4921 accacggtag gctgcgaaat gcccatactg ttgagcaatt cacgttcatt ataaatgtcg
     4981 ttcgcgggcg caactgcaac tccgataaat aacgcgccca acaccggcat aaagaattga
     5041 agagagtttt cactgcatac gacgattctg tgatttgtat tcagcccata tcgtttcata
     5101 gcttctgcca accgaacgga catttcgaag tactcagcgt aagtgatgtc cacctcgata
     5161 tgtgcatctg taaaagcaat tgttccagga accagggcgt atctcttcat agccttatgc
     5221 agttgctctc cagcggttcc atcttccagc ggatagaatg gcgccgggcc tttctttatg
     5281 tttttggcgt cttccatggt ggctttacca acagtaccgg aatgccaagc tttttgcaaa
     5341 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     5401 cctcggcctc tgcataaata aaaaaaatta gtcagcgatg gggcggagaa tgggcggaac
     5461 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     5521 tgagatgcag atcgcagatc
//
