LOCUS       sequence-46681-e        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN41-R4R3
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1158..1186)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1934..1954
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3016..3035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3614..3634
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3909..4703
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3909..4703
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3963..3982)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4573..4592
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5278..5300
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5338..5356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5424..5528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5529..5597,5598..6389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5747..5766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6560..7148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7049..7068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7274..7296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7293..7309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7293..7309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    7329..7452
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7493..7798)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7595..7614)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8118..8801)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     8716..8735
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8855..8861,8862..8879,8880..8885))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(8910..9033)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9041..9058)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9041..9057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9050..9072)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
       61 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      121 ccttacgcat ctgtgcggta tttcacaccg catatgccgg gccccatttt atatctgagt
      181 agtatccttt gctttaaatg tccataaaac taattttata atcaataaaa caacgtttgt
      241 aaatcaactg agtttacaag tagagacatt gagggatact ttcactatgc taaagtgata
      301 atcgaccaaa taataacttc actttggtat ttattcctgt cttataaatg ttatgtatga
      361 attaaattca tatgcatatg gctcactctg acaattttta ataatcttcc agatcaatat
      421 tgactaccga tgcgggtggt cttttgcttt gaattctgct gaactttaca ccccgaacag
      481 caatgtgtgc ttcagcctaa aaaaaagtaa gtgtgttaat cagtgccccc gattcttcat
      541 tttttgcccc tctctcccgt ttcgtcggca aaagaagaga aaataaagat aagtctcaag
      601 ataggttggt aatcgctaaa gtggttgtgt ggataagagt agcaaaatgg caggaagagc
      661 actttgcgcg cacacactgt actcattgtt ctggataaaa ttctctcgtt gtttgccgtc
      721 ggatgtctgc ctctctgcat tgagccggct tcttcactat ctttagttaa cctaaaatgc
      781 cgtttctttt ctcgtatccc cactatcccg ttgaggttct ctgctctctt cgctccctac
      841 cgccagcgag caactatccg tgggggcgcc ttgctcggaa gatggggggg aagaaagaag
      901 atttttgcta tttgcacttg agaaagagac ttttcctgcg tcgatggtta gagaacagtg
      961 tgcagacact tttcagctac ctagaattac aattggatat ccccgcctcc caatccaccc
     1021 acccagggaa aaagaagggc tcgccgaaaa tcaaagttat ctccaggctc gcgcatccca
     1081 ccgagcggtt gacttctctc caccactttt catttttacc ctcgaccatg agtaaaggag
     1141 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1201 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1261 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac
     1321 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg
     1381 ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     1441 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1501 cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg
     1561 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1621 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1681 acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac
     1741 taactaacta atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg
     1801 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1861 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     1921 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     1981 atgaactata caaatagatc aactgctcta attccatctc gtttctctct tttctttcaa
     2041 tctctttacc cgtttctctt cccctacgtc ccccaaacct accactgcac agtaacacat
     2101 acacattcaa cgtgtcacaa taaaagccca tcaacacctt ctctgttgtt ttttttcttc
     2161 aaatgcattt ttttcacttt gtttaataat ttttacgcat cggatttgta ggatagtgga
     2221 aatttgtcct tcaatgcttc aattgattcg acacttctca atatttttga tcaataaacc
     2281 attgattttc tatttagaag aagatcactg taaataacac caaaaacatc acagcaaaaa
     2341 aactcccact caattatatt actgttttta ttgaaactag aataaatttg aagggccccc
     2401 ctcgagggga gaaaatgcgg agagaagaac ccagcatctt aataaaatta tataattttg
     2461 aataaaatca aattaaaaat aagattaatt gaaacacaac aatagaatgc agaaaattac
     2521 tcataagaaa taatttattt tgaaagaagt tgtgtcgatt tacgataaaa actacacgca
     2581 aacgaggctc cgcgtttaca ccgtttggca atggagcgcg cttgctatta gattttaggc
     2641 cagctcaacg aaagtttaat ttatttttct tctttttttc catttttttg agtaaaatat
     2701 cgaatttttt gtattaattc tgtaaatttt ataatttgca actattttgg cgtattctgc
     2761 ttgtttttat cagcgaattt ttatgcattt cagtcaaaag tttgagcagg aaaaatgaca
     2821 tttaggactt tgtaaaattg tattcttaaa tttgcttgaa aacctacgtg tacaattgat
     2881 tttattgaat ttttcttacg tttcccgttg tatcttattg tatttaactt tcatcataaa
     2941 ataatttatt tccaggtaaa atatccgacg ctctcgtgga tccccgggat tggctcagaa
     3001 aaaaaggtac cgaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3061 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3121 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3181 cgcgtcgggc tcgacatcgg caaggtgtgg gtcggtaagt ttaaacatat atatactaac
     3241 taaccctgat tatttaaatt ttcagcggac gacggcgccg cggtggcggt ctggaccacg
     3301 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     3361 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     3421 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgacgtaag tttaaacatg
     3481 attttactaa ctaactaatc tgatttaaat tttcagcacc agggcaaggg tctgggcagc
     3541 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     3601 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     3661 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatga
     3721 gctagcgatt actgaaatat gtaaatatta gaagaatttg tgcgggattc ttgccaaata
     3781 aattctcgaa atagttttca tatatttatt gttatgcgcg ttctctcgct tcgatttatc
     3841 tctcttcctt tactctcgtg tatttctaaa ctttcccctt cttatcagta tttatttcag
     3901 ggtaaacgat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4681 gccttcttga cgagttcttc tgagctagcc ggccatacaa gtaatccgga tgatcgacgc
     4741 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
     4801 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
     4861 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     4921 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     4981 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     5041 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     5101 gaaaactagt ccaagatctc agtggtccta ggtgagctcc catatggtgc actctcagtg
     5161 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5221 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5281 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5341 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggcctttgc
     7201 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7261 gtgagctgat accagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7321 caagctatca actttgtata gaaaagttga acgagaaacg taaaatgata taaatatcaa
     7381 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     7441 ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc
     7501 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     7561 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
     7621 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
     7681 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
     7741 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
     7801 caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
     7861 gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc
     7921 tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat
     7981 agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat
     8041 acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc
     8101 atactgttat ctggctttta gtaagccgga tccacgcggc gtttacgccc cccctgccac
     8161 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     8221 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     8281 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     8341 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     8401 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     8461 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     8521 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     8581 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     8641 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     8701 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     8761 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     8821 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt
     8881 gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
     8941 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     9001 acgtttctcg ttcaacttta ttatacatag ttgataattc actggccgtc gttttacaac
     9061 gtcgtgactg ggaaaaccct ggcgttaccc aacttaa
//
