LOCUS       sequence-466942-        6155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing HSP90AB1 shRNA in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-RedO-shHsp90ab1
            (#a)
FEATURES             Location/Qualifiers
     source          1..6155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1356..1376)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1774..1785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1852..1869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1858..2065
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2153..2282
                     /label=AAV2 ITR
     primer_bind     complement(2301..2318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2301..2317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2310..2332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2459..2914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2546..2565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2756..2777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2940..3044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3045..3113,3114..3905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3263..3282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4076..4664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4565..4584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4952..4973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4988..4993,4994..5011,5012..5018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5026..5042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5031..5053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5050..5066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5050..5066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5104..5233
                     /label=AAV2 ITR
ORIGIN
        1 catcccaccc cctcaccccc tcgttcttca tatccttctc tagtgctccc tccactttca
       61 tccacccttc tgcaagagtg tgggaccaca aatgagtttt cacctggcct ggggacacac
      121 gtgcccccac aggtgctgag tgactttcta ggacagtaat ctgctttagg ctaaaatggg
      181 acttgatctt ctgttagccc taatcatcaa ttagcagagc cggtgaaggt gcagaaccta
      241 ccgcctttcc aggcctcctc ccacctctgc cacctccact ctccttcctg ggatgtgggg
      301 gctggcacac gtgtggccca gggcattggt gggattgcac tgagctgggt cattagcgta
      361 atcctggaca agggcagaca gggcgagcgg agggccagct ccggggctca ggcaaggctg
      421 ggggcttccc ccagacaccc cactcctcct ctgctggacc cccacttcat agggcacttc
      481 gtgttctcaa agggcttcca aatagcatgg tggccttgga tgcccaggga agcctcagag
      541 ttgcttatct ccctctagac agaaggggaa tctcggtcaa gagggagagg tcgccctgtt
      601 caaggccacc cagccagctc atggcggtaa tgggacaagg ctggccagcc atcccaccct
      661 cagaagggac ccggtggggc aggtgatctc agaggaggct cacttctggg tctcacattc
      721 ttggatccgg ttccaggcct cggccctaaa tagtctccct gggctttcaa gagaaccaca
      781 tgagaaagga ggattcgggc tctgagcagt ttcaccaccc accccccagt ctgcaaatcc
      841 tgacccgtgg gtccacctgc cccaaaggcg gacgcaggac agtagaaggg aacagagaac
      901 acataaacac agagagggcc acagcggctc ccacagtcac cgccaccttc ctggcgggga
      961 tgggtggggc gtctgagttt ggttcccagc aaatccctct gagccgccct tgcgggctcg
     1021 cctcaggagc aggggagcaa gaggtgggag gaggaggtct aagtcccagg cccaattaag
     1081 agatcaggta gtgtagggtt tgggagcttt taaggtgaag aggcccgggc tgatcccaca
     1141 ggccagtata aagcgccgtg accctcaggt gatgcgccag ggccggctgc cgtcggggac
     1201 agggctttcc atagcgatcc ggcatctacc gcatgattaa actcaagagg tttaatcatg
     1261 cggtagatgc ttttttggaa ttcgcggccc tagcttggat ccaatcaacc tctggattac
     1321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1441 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1501 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1561 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     1621 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     1681 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     1741 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     1801 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgagatct gcctcgactg
     1861 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1921 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1981 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2041 aagacaatag caggcatgct ggggactcga gttaagggcg aattcccgat aaggatcttc
     2101 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     2161 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2221 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2281 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2761 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2881 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3061 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3121 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3181 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3241 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3301 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3361 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3421 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3481 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3541 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3601 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3661 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4741 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4801 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4861 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4921 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4981 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5041 aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa ggccttaatt
     5101 aggctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
     5161 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     5221 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
     5281 tctaggaaga tcggaattcg cccttaagct agcctacagc agccagggtg agattatgag
     5341 gctgagctga gaatatcaag actgtaccga gtagggggcc ttggcaagtg tggagagccc
     5401 ggcagctggg gcagagggcg gagtacggtg tgcgtttacg gacctcttca aacgaggtag
     5461 gaaggtcaga agtcaaaaag ggaacaaatg atgtttaacc acacaaaaat gaaaatccaa
     5521 tggttggata tccattccaa atacacaaag gcaacggata agtgatccgg gccaggcaca
     5581 gaaggccatg cacccgtagg attgcactca gagctcccaa atgcatagga atagaagggt
     5641 gggtgcagga ggctgagggg tggggaaagg gcatgggtgt ttcatgagga cagagcttcc
     5701 gtttcatgca atgaaaagag tttggagacg gatggtggtg actggactat acacttacac
     5761 acggtagcga tggtacactt tgtattatgt atattttacc acgatctttt taaagtgtca
     5821 aaggcaaatg gccaaatggt tccttgtcct atagctgtag cagccatcgg ctgttagtga
     5881 caaagcccct gagtcaagat gacagcagcc cccataactc ctaatcggct ctcccgcgtg
     5941 gagtcattta ggagtagtcg cattagagac aagtccaaca tctaatcttc caccctggcc
     6001 agggccccag ctggcagcga gggtgggaga ctccgggcag agcagagggc gctgacattg
     6061 gggcccggcc tggcttgggt ccctctggcc tttccccagg ggccctcttt ccttggggct
     6121 ttcttgggcc gccactgctc ccgctcctct ccccc
//
