LOCUS       sequence-467134-        9867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication 
            origin and Nat selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM01
FEATURES             Location/Qualifiers
     source          1..9867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             810..821
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             828..1541
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1553..1624
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1640..1667
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        1681..1756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1890..2232
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2812..3009
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(3160..3951,3952..4020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3783..3802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4021..4125)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4828..8931
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4828..8931
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8941..8961
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8977..9000
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      9004..9193
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(9250..9838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9330..9349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg
       61 agctctttgc acttctttag ttctttccat caatatcagt tttttaaact tttaggacta
      121 aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat
      181 actaatacac tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa
      241 aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt
      301 agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac
      361 atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca
      421 tgtatataaa cgtcaaatca taatcagcac tagtatatgt gttatgtagt atactctttc
      481 ttcaacaatt aaatactctc ggtagccaag ttggtttaag gcgcaagact gtaatttatc
      541 actacgaaat cttgagatcg ggcgttcgac tcgcccccgg gagagatggc cggcatggtc
      601 ccagcctcct cgctggcgcc ggctgggcaa caccttcggg tggcgaatgg gactcgagac
      661 cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      721 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
      781 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
      841 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      901 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      961 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1021 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1081 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1141 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1201 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1261 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1321 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1381 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1441 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1501 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1561 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1621 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctcc
     1681 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1741 ggcaccgagt cggtgctttt tttatttttt gtcactattg ttatgtaaaa tgccacctct
     1801 gacagtatgg aacgcaaact tctgtctagt ggatataggt ctagagatct gtttagcttg
     1861 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
     1921 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
     1981 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     2041 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     2101 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     2161 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     2221 aacataaaca acaatgggta ctaccttaga tgatacagcc tacagataca gaacatcagt
     2281 ccctggtgat gctgaagcaa ttgaggcttt agacggttca ttcaccaccg acaccgtctt
     2341 tagagtaacc gccaccggtg atggatttac cttaagagaa gtcccagtcg accctccatt
     2401 aactaaagtc tttccagatg atgaatctga tgacgaaagc gacgacggag aagatggtga
     2461 cccagattca agaactttcg tagcatacgg tgatgacggt gatttggctg gttttgtagt
     2521 cgtttcttat tcaggttgga atagaaggtt gaccgttgaa gatatagaag tcgccccaga
     2581 gcatagaggt catggtgtag gaagagcttt gatgggtttg gctacagaat ttgcaagaga
     2641 gagaggagcc ggtcatttat ggttagaagt tactaatgtt aacgcccctg ctatccatgc
     2701 ttatagaaga atgggtttca cattatgtgg tttagatact gctttatatg atggaacagc
     2761 atctgacggt gaacaggcct tgtatatgtc tatgccttgc ccttaatcag tactgacaat
     2821 aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt
     2881 ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca
     2941 gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc
     3001 gctatactgc tgtcgattcg atactaacgc cgccatccag tgtcgaaaac gagctctcga
     3061 gaacccttaa tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3301 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4081 catatttgaa tgtatttaga aaaataaaca aataggggtt cgtcgacagt cgaacaagaa
     4141 gcaggcaaag tttagagcac tgcccctccg cactcaaaaa agaaaaaact aggaggaaaa
     4201 taaaattctc aaccacacaa acacataaac acatacaaac acaaatacaa gcttatttac
     4261 ttgacatcgc gcgatcttcc actattcagc gccgtccgcc ctctctcgtg ttttttgttt
     4321 acgcgacaac tatgcgaaat ccggagcaac gggcaaccgt ttggggaaag accacaccca
     4381 cgcgcgatcg ccatggcaac gaggtcgcac acgccccaca cccagacctc cctgcgagcg
     4441 ggcatgggta caatgtcccc gttgccacag acaccacttc gtagcacagc gcagagcgta
     4501 gcgtgttgtt gctgctgaca aaagaaaatt tttcttagca aagcaaagga ggggaagcac
     4561 gggcagatag caccgtacca tacccttgga aactcgaaat gaacgaagca ggaaatgaga
     4621 gaatgagagt tttgtaggta tatatagcgg tagtgtttgc gcgttaccat catcttctgg
     4681 atctatctat tgttcttttc ctcatcactt tccccttttt cgctcttctt cttgtctttt
     4741 atttctttct tttttttaat tgttccctcg attggctatc taccaaagaa tccaaactta
     4801 atacacgtat ttatttgtcc aattaccatg gataagaaat actcaatagg cttagatatc
     4861 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
     4921 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
     4981 ttatttgaca gtggagagac agcggaagcg actcgactca aacggacagc tcgtagaagg
     5041 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
     5101 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
     5161 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
     5221 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
     5281 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
     5341 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
     5401 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
     5461 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
     5521 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
     5581 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
     5641 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
     5701 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     5761 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     5821 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     5881 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     5941 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     6001 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
     6061 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     6121 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
     6181 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     6241 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     6301 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     6361 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     6421 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     6481 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     6541 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     6601 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     6661 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     6721 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     6781 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     6841 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     6901 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     6961 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
     7021 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
     7081 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
     7141 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
     7201 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
     7261 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
     7321 cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa agacgattca
     7381 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
     7441 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
     7501 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
     7561 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
     7621 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
     7681 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
     7741 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
     7801 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
     7861 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
     7921 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
     7981 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
     8041 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
     8101 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta
     8161 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
     8221 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     8281 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
     8341 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     8401 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     8461 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     8521 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     8581 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     8641 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     8701 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     8761 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     8821 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     8881 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga cggtggaggg
     8941 ccaaaaaaga aaagaaaagt tgaagatgct tctgctcatc atcaccatca ccatcaccat
     9001 taaatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     9061 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     9121 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     9181 gggacgctcg aagtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9841 agatcctttg atcttttcta cactagt
//
