LOCUS       sequence-467229-       10712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd or
            3rd generation lentiviral vectors and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10712)
  TITLE     Trono Lab Packaging and Envelope Plasmids
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: psPAX2
FEATURES             Location/Qualifiers
     source          1..10712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..686
                     /label=chicken beta-actin promoter
     intron          687..1703
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1627..1649
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1711..1730
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1809..3311
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3104..6115
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5800..5917
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6689..6922
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7107..7151
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7445..7471
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(7756..7775)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7821..7876
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7875..7894)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8237..8253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8237..8253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8250..8272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8261..8277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8285..8291,8292..8309,8310..8315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8330..8351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8410..8605
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8456..8591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8611..8745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8648..8667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8702..8721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8813..8830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8984..9572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9064..9083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9743..10534,10535..10603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10366..10385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10604..10708)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      421 cacgttctgc ttcactctcc ccatctcccc ccccctcccc acccccaatt ttgtatttat
      481 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag
      541 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
      601 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
      661 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
      721 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      781 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
      841 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
      901 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
      961 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     1021 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     1081 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     1141 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     1201 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     1261 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     1321 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     1381 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     1441 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     1621 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     1681 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     1741 attttggcaa agaattcggg ccgcgttgac gcgcacggca agaggcgagg ggcggcgact
     1801 ggtgagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgatgggaaa
     1861 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1921 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1981 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     2041 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     2101 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaaaaaa gcacagcaag
     2161 cagcagctga cacaggacac agcaatcagg tcagccaaaa ttaccctata gtgcagaaca
     2221 tccaggggca aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag
     2281 tagtagaaga gaaggctttc agcccagaag tgatacccat gttttcagca ttatcagaag
     2341 gagccacccc acaagattta aacaccatgc taaacacagt ggggggacat caagcagcca
     2401 tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga atgggataga gtgcatccag
     2461 tgcatgcagg gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag
     2521 gaactactag tacccttcag gaacaaatag gatggatgac acataatcca cctatcccag
     2581 taggagaaat ctataaaaga tggataatcc tgggattaaa taaaatagta agaatgtata
     2641 gccctaccag cattctggac ataagacaag gaccaaagga accctttaga gactatgtag
     2701 accgattcta taaaactcta agagccgagc aagcttcaca agaggtaaaa aattggatga
     2761 cagaaacctt gttggtccaa aatgcgaacc cagattgtaa gactatttta aaagcattgg
     2821 gaccaggagc gacactagaa gaaatgatga cagcatgtca gggagtgggg ggacccggcc
     2881 ataaagcaag agttttggct gaagcaatga gccaagtaac aaatccagct accataatga
     2941 tacagaaagg caattttagg aaccaaagaa agactgttaa gtgtttcaat tgtggcaaag
     3001 aagggcacat agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg aaatgtggaa
     3061 aggaaggaca ccaaatgaaa gattgtactg agagacaggc taatttttta gggaagatct
     3121 ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc
     3181 caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggagc
     3241 cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccct
     3301 cgtcacaata aagatagggg ggcaattaaa ggaagctcta ttagatacag gagcagatga
     3361 tacagtatta gaagaaatga atttgccagg aagatggaaa ccaaaaatga tagggggaat
     3421 tggaggtttt atcaaagtaa gacagtatga tcagatactc atagaaatct gcggacataa
     3481 agctataggt acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt
     3541 gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa
     3601 attaaagcca ggaatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat
     3661 aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg
     3721 gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg
     3781 gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct gggaagttca
     3841 attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt
     3901 gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac
     3961 catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca
     4021 gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt
     4081 tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt atgtaggatc
     4141 tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag
     4201 gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg
     4261 ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag
     4321 ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta
     4381 tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga
     4441 agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa
     4501 agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa
     4561 gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac
     4621 aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc
     4681 agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt
     4741 acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat
     4801 tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa
     4861 agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa
     4921 attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga
     4981 cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt
     5041 agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag cacaaccaga
     5101 taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt
     5161 ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt
     5221 ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga
     5281 acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc taccacctgt
     5341 agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca
     5401 tggacaagta gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa
     5461 agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc
     5521 agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa
     5581 aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg
     5641 gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat
     5701 agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca
     5761 tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     5821 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     5881 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5941 agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat
     6001 acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta
     6061 tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacacat
     6121 ggaattctgc aacaactgct gtttatccat ttcagaattg ggtgtcgaca tagcagaata
     6181 ggcgttactc gacagaggag agcaagaaat ggagccagta gatcctagac tagagccctg
     6241 gaagcatcca ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta aaaagtgttg
     6301 ctttcattgc caagtttgtt tcatgacaaa agccttaggc atctcctatg gcaggaagaa
     6361 gcggagacag cgacgaagag ctcatcagaa cagtcagact catcaagctt ctctatcaaa
     6421 gcagtaagta gtacatgtaa tgcaacctat aatagtagca atagtagcat tagtagtagc
     6481 aataataata gcaatagttg tgtggtccat agtaatcata gaatatagga aaatggccgc
     6541 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6601 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6661 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     6721 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     6781 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     6841 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     6901 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     6961 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7021 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7081 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7141 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7201 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7261 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7321 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     7381 ttcgattagt gaacggatcc ttggcactta tctgggacga tctgcggagc ctgtgcctct
     7441 tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg
     7501 gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacaatat tggagtcagg
     7561 agctaaagaa tagtgctgtt agcttgctca atgccacagc catagcagta gctgagggga
     7621 cagatagggt tatagaagta gtacaaggag cttgtagagc tattcgccac atacctagaa
     7681 gaataagaca gggcttggaa aggattttgc tataagctcg aaacaaccgg tacctctaga
     7741 actatagcta gcagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     7801 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     7861 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     7921 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     7981 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     8041 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     8101 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     8161 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     8221 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8401 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8461 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8521 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8581 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     8641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     8761 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8881 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8941 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9001 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9061 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9121 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9181 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9241 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9301 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9361 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9421 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9481 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9541 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9601 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9661 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9721 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9781 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9841 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9901 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9961 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10021 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10081 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10141 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10201 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10261 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10321 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10381 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10441 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10501 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10561 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10621 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10681 agaaaaataa acaaataggg gttccgcgca ca
//
