LOCUS       sequence-467291-        5892 bp    DNA     circular SYN 12-FEB-2026
DEFINITION  ReaChR-citrine in AAV2 vector under human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Lin JY, Knutsen PM, Muller A, Kleinfeld D, Tsien RY
  TITLE     ReaChR: a red-shifted variant of channelrhodopsin enables deep 
            transcranial optogenetic excitation.
  JOURNAL   Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 
            2013 Sep 1.
  PUBMED    23995068
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3502"; 
            journalName: "Nat Neurosci"; date: "2013-10"; volume: "16"; issue: 
            "10"; pages: "1499-508"
COMMENT     Sequence Label: AAV-ReaChR-citrine
FEATURES             Location/Qualifiers
     source          1..5892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    319..440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(356..375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     410..429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     469..485
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     470..486
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    489..1077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             595..606
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1004..1024
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1093..1803,1804..1806,1807..1809))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1135..1156)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1484..1503
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1743..1764
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2897..3344)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   3426..3555
                     /label=AAV2 ITR
     rep_origin      3627..4082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3714..3733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3924..3945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4108..4212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4213..4281,4282..5073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4431..4450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5244..5832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5733..5752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga gcgagcgcgc
      121 agagagggag tggccaactc catcactagg ggttccttgt agttaatgat taacccgcca
      181 tgctacttat ctacgtagcc atgctctagg aagatcctta tcgattttac cacatttgta
      241 gaggttttac ttgctttaaa aaacctccca catctccccc tgaacctgaa acataaaatg
      301 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      421 aaactcatca atgtatctta tcatgtctgc tcgaagcggc cgcccgggtc gaggtcgacg
      481 gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
      541 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
      601 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
      661 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
      721 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
      781 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
      841 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
      901 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
      961 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     1021 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
     1081 gataagcttc tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     1141 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
     1201 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     1261 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     1321 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     1381 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     1441 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     1501 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     1561 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac atcaggccgt agccgaaggt
     1621 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     1681 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     1741 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     1801 gctcaccatc tcgaggctgc tctcgtactt atcttcttct tccgccacca gggtttccac
     1861 ttccatttcc tggcccgcaa tggtaatttt ctgttttttg cgaatatcgc catacagcag
     1921 aatatgttca tgaattttca cgcgcagata gttgcccagc acgccccaca tgttcttcgc
     1981 aatcagatcc agaatgctat ggccaatcgc gctgccatac gggctaatat ggccgaagcc
     2041 ctcggggccc agcaggaaca gcacggggaa catgccccag ctcacgaaga acagccaggc
     2101 catggctctc accagctgtc tgcacaggcc tttcggcacg gtatgaaacg cttcaatata
     2161 cactttggcc gcatgaaaat aggtatacat gccatagctc aggctaatca gaaaaaacag
     2221 aattttggtc cagccggtgc acatcgcgct ggtcgcgccc cacacaatgc agcccacgtc
     2281 gctcaccagc aggcccatgg tgcgtttgct ataatcatct ttcaggccgg tcaggttgct
     2341 cagatgaatc agaatcacgg ggcaggtcag cagccattcg ccatagcgca tccacaccac
     2401 gccgttgccg ctgctcagcc acagggtcgc cgggctatca aattcatgaa acgcttcaat
     2461 aatgcttttc atcatttcaa tcagcgccac atacacttct tcccagccgc aggtcgcgcg
     2521 ccacgcctga tacgcatacc agcccaggca cgccacgctc agcgcaaaca ccacccactg
     2581 caggatgttg gcggccagct tctcggcgtt ggtgccgtta ctcttcagcc aggccaggca
     2641 gaagcactgg ccgttgttgg ggatgcagat cacgctgccg ttgttctcca gggtgtagct
     2701 ggtctggaac agcattctct cgtgggctct gtggaacacg tagtcggggc cgtcctgggt
     2761 ggccacgggc acggtggcgt cgctgccggt gctggcgccg gcgctgccgg cggccagggc
     2821 cacggccagg gccagggcca gcagccaggg tcttctgctc accatggtcg gatccttgct
     2881 agcagcttga attcgactgc gctctcaggc acgacacgac tcctccgctg cccaccgcag
     2941 actgaggcag cgctgagtcg ccggcgccgc agcgcagatg gtcgcgcccg tgccccccta
     3001 tctcgcgcct cgcgtggtgc ggtccggctg ggccggcggc ggcgcggacg cgaccaaggt
     3061 ggccgggaag gggagtttgc gggggaccgg cgagtgacgt cagcgcgcct tcagtgctga
     3121 ggcggcggtg gcgcgcgccg ccaggcgggg gcgaaggcac tgtccgcggt gctgaagctg
     3181 gcagtgcgca cgcgcctcgc cgcatcctgt ttcccctccc cctctctgat aggggatgcg
     3241 caatttgggg aatgggggtt gggtgcttgt ccagtgggtc ggggtcggtc gtcaggtagg
     3301 cacccccacc ccgcctcatc ctggtcctaa aacccacttg cactcatacg cagggccctc
     3361 tgcagagatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     3421 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3481 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3541 cgagcgagcg cgcagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3601 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3901 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3961 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4021 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4081 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4441 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4741 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5881 tttgctcaca tg
//
