LOCUS       sequence-467435-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress ADAR2dd-MCP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(822..926)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(952..1407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1089..1110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1301..1320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1534..1556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1548..1565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1549..1565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1695..1757
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2340..3518
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2571..3509
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3466..3486
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5507..5560
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5567..6163
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5567..5586)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6063..6083
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6164..6220
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6227..6937
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(6971..6988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6977..7184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7480..7514
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8005..8021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8005..8021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8018..8040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8029..8045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8053..8059,8060..8077,8078..8083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8098..8119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8236..8253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8407..8995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8487..8506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9166..9205,1..752,753..821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
       61 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
      961 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1021 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1081 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1141 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1201 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1261 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1321 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1381 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1561 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1621 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1681 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     1741 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     1801 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     1861 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     1921 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     1981 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2041 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2101 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2161 aaaaggcctc cacggccact agtttcgcac gattgcacct tgggagcctt aaccgctttt
     2221 gaatgtccgt cttagttaat ccctaattga acgctccaaa aggtgacttc caatagggat
     2281 ttattccttt taaaattaac ggcataatcg tgccgccgaa tgcaacctcg atcgagccgg
     2341 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2401 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2461 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2521 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     2581 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     2641 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     2701 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     2761 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     2821 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     2881 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     2941 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3001 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3061 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3121 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3181 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3241 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3301 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3361 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3421 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3481 tggttcaaag tttttttctt ccatttcagg tgtcgtgaat ctagaagcca ccatgttgca
     3541 cttggatcaa acgccatctc gccagcctat tcccagtgag ggtcttcagc tgcatttacc
     3601 gcaggtttta gctgacgctg tctcacgcct ggtcctgggt aagtttggtg acctgaccga
     3661 caacttctcc tcccctcacg ctcgcagaaa agtgctggct ggagtcgtca tgacaacagg
     3721 cacagatgtt aaagatgcca aggtgataag tgtttctaca ggaacaaaat gtattaatgg
     3781 tgaatacatg agtgatcgtg gccttgcatt aaatgactgc catgcagaaa taatatctcg
     3841 gagatccttg ctcagatttc tttatacaca acttgagctt tacttaaata acaaagatga
     3901 tcaaaaaaga tccatctttc agaaatcaga gcgagggggg tttaggctga aggagaatgt
     3961 ccagtttcat ctgtacatca gcacctctcc ctgtggagat gccagaatct tctcaccaca
     4021 tgagccaatc ctggaagaac cagcagatag acacccaaat cgtaaagcaa gaggacagct
     4081 acggaccaaa atagagtctg gtcaggggac gattccagtg cgctccaatg cgagcatcca
     4141 agcgtgggac ggggtgctgc aaggggagcg gctgctcacc atgtcctgca gtgacaagat
     4201 tgcacgctgg aacgtggtgg gcatccaggg atccctgctc agcattttcg tggagcccat
     4261 ttacttctcg agcatcatcc tgggcagcct ttaccacggg gaccaccttt ccagggccat
     4321 gtaccagcgg atctccaaca tagaggacct gccacctctc tacaccctca acaagccttt
     4381 gctcagtggc atcagcaatg cagaagcacg gcagccaggg aaggccccca acttcagtgt
     4441 caactggacg gtaggcgact ccgctattga ggtcatcaac gccacgactg ggaaggatga
     4501 gctgggccgc gcgtcccgcc tgtgtaagca cgcgttgtac tgtcgctgga tgcgtgtgca
     4561 cggcaaggtt ccctcccact tactacgctc caagattacc aaacccaacg tgtaccatga
     4621 gtccaagctg gcggcaaagg agtaccaggc cgccaaggcg cgtctgttca cagccttcat
     4681 caaggcgggg ctgggggcct gggtggagaa gcccaccgag caggaccagt tctcactcac
     4741 gcccggtacc ggtggcggaa ccggtggcgg ggctatggcg agcaacttta cgcaatttgt
     4801 gttggttgac aatggtggca ccggagacgt aactgttgcg ccgagcaatt ttgcgaacgg
     4861 gattgcagaa tggataagta gtaacagccg gagtcaggca tataaagtca catgctcagt
     4921 tcgccaaagt tccgcacaga accgaaaata taccataaag gtagaagtgc caaaaggggc
     4981 gtggcgatct tatcttaata tggagcttac gatcccaatc tttgcgacta attcagactg
     5041 cgagctgatt gtcaaggcca tgcaaggtct tttgaaagac ggtaacccga ttccctctgc
     5101 gatcgccgcc aactcaggaa tatatggtgg cggaaccggt ggcgggtcca tggcctccaa
     5161 ttttacacag tttgtgctcg tggacaacgg cggcactgga gacgtcacag tagctccaag
     5221 taactttgcg aatgggatag ccgagtggat ctcttccaat tcaagatcac aagcctataa
     5281 ggtgacttgt agtgtacgcc aatctagtgc ccagaaccgg aagtatacca ttaaagtgga
     5341 ggtccccaaa ggtgcatggc gctcatactt gaatatggaa ttgacgattc cgatatttgc
     5401 gaccaatagc gactgcgagc ttattgtaaa agccatgcaa ggacttctga aagatggcaa
     5461 cccgatacct tccgcaatcg ctgcgaatag cggaatttac gctagcgaag gtcgggggag
     5521 tttgcttacc tgtggcgatg tcgaggagaa tccagggccc aagcttatga ccgagtacaa
     5581 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5641 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5701 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5761 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5821 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5881 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5941 ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6001 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6061 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6121 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgccacga acttctctct
     6181 gttaaagcaa gcaggtgacg tggaagaaaa ccccggtccc ggatccatgg tgtctaaggg
     6241 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa
     6301 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     6361 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
     6421 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     6481 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt
     6541 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     6601 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     6661 ggccttcacc gaaacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
     6721 cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa
     6781 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
     6841 aatcaaggag gccaacaacg aaacctacgt cgagcagcac gaggtggcag tggccagata
     6901 ctgcgacctc cctagcaaac tggggcacaa acttaattaa gaattcctcg agctagagct
     6961 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7021 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7081 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7141 agcaaggggg aggattggga agacaatagc aggcatgctg gggacgacgt caggtggcac
     7201 ttttcgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     7261 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     7321 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     7381 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     7441 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     7501 ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct tttttccggc
     7561 tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt
     7621 cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg
     7681 ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta
     7741 acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac
     7801 agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga
     7861 actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg
     7921 aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg
     7981 gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9181 cagtgaggca cctatctcag cgatc
//
