LOCUS       sequence-467559-        2316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_pMB1_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK017 [Origin_pMB1_PartType1]
FEATURES             Location/Qualifiers
     source          1..2316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        659..750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(751..819,820..1611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1615..1654
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1682..2270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgcaattg agaccagagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
      661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      721 atgcttcaat aatattgaaa aaggaagagt atgagcattc agcattttcg tgtggcgctg
      781 attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa
      841 gtgaaagatg cggaagatca actgggtgcg cgcgtgggct atattgaact ggatctgaac
      901 agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt
      961 aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga acaactgggc
     1021 cgtcgtattc attatagcca gaacgatctg gtggaatata gcccggtgac cgaaaaacat
     1081 ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg cgattaccat gagcgataac
     1141 accgcggcga acctgctgct gacgaccatt ggcggtccga aagaactgac cgcgtttctg
     1201 cataacatgg gcgatcatgt gacccgtctg gatcgttggg aaccggaact gaacgaagcg
     1261 attccgaacg atgaacgtga taccaccatg ccggcagcaa tggcgaccac cctgcgtaaa
     1321 ctgctgacgg gtgagctgct gaccctggca agccgccagc aactgattga ttggatggaa
     1381 gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc cggctggctg gtttattgcg
     1441 gataaaagcg gtgcgggcga acgtggcagc cgtggcatta ttgcggcgct gggcccggat
     1501 ggtaaaccga gccgtattgt ggtgatttat accaccggca gccaggcgac gatggatgaa
     1561 cgtaaccgtc agattgcgga aattggcgcg agcctgatta aacattggta aaccgataca
     1621 attaaaggct ccttttggag cctttttttt tggacgaccc ttgtcagatc aaaggatctt
     1681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2281 acgcgagtcg gtctctccga agatcaaagg atcttc
//
