LOCUS       sequence-46767-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72019
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(127..144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(206..230)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        277..381
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(600..619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttgcaagg actgaagtgg aattcatgag atctgggaca atcccggtct ttaagaacag
       61 gaattacatg tctgacgatt acaaggatga cgacgacaag ggatcctaac tcgctcctca
      121 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      181 tcactcaaag gcggtaatca attcgaccca gctttcttgt acaaagtggt tgatcggatc
      241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      481 gcagaggatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      601 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt tgcacaacat
      841 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      901 cgacgagcgt ggacaccacg aa
//
