LOCUS       sequence-467733-       11075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized T4 DNA Ligase with N-terminal E1 MLS 
            expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, 
            DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, 
            Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
  TITLE     Engineering mtDNA Deletions by Reconstituting End-Joining in Human 
            Mitochondria.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543.
  PUBMED    39463974
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543."
COMMENT     Sequence Label: pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato
FEATURES             Location/Qualifiers
     source          1..11075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1346..1934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1399..1419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1817..1828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2082..2262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2309..2327)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2395..2499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2500..2568,2569..3360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2718..2737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3531..4119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4273..4290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4530..4551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4566..4571,4572..4589,4590..4596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4604..4620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4609..4631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4628..4644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4628..4644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4694..4713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4905..5538
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5585..5710
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6207..6440
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6625..6669
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6962..7079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7127..8305
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7358..8296
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8253..8273
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8592..8639
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     RBS             9740..9748
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    10396..10963
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    10413..10965
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10557..10574)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10784..10803
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(10977..11075,1..1332)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
       61 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
      121 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
      181 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
      241 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
      301 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
      361 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
      421 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
      481 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
      541 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
      601 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag
      661 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      721 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      781 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      841 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      901 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      961 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     1021 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     1081 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     1141 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     1201 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     1261 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
     1321 ctgtacaagt aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt
     1381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     1441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     1501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     1561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     1621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     1681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     1741 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     1801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     1861 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     1921 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     1981 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     2041 atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc tggttagacc
     2101 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2161 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2221 gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg attaaggaaa
     2281 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2341 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2401 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2701 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     2761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3661 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4321 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
     4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
     4501 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4561 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4621 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
     4681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     4741 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     4801 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     4861 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     4921 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4981 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     5041 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     5101 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     5161 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     5221 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     5281 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     5341 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5401 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5461 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5521 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     5581 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     5641 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     5701 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     5761 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     5821 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     5881 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     5941 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     6001 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     6061 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6121 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6181 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6241 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6301 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6361 caactcacag tctggggcat caagcagctt caggcaagaa tcctggctgt ggaaagatac
     6421 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6481 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6541 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6601 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6661 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6721 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6781 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6841 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6901 cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca
     6961 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     7021 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     7081 gggtttatta cagggacagc agagatccag tttggactag tcgtgaggct ccggtgcccg
     7141 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     7201 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     7261 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     7321 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     7381 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     7441 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     7501 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     7561 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     7621 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     7681 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     7741 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     7801 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     7861 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     7921 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     7981 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     8041 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     8101 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     8161 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     8221 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     8281 ttttcttcca tttcaggtgt cgtgaagcgg ccgcttaatt aagtttctcg cggtgccctt
     8341 gcggcgcagc tggggtcgcg gccctgctcc ccgcgctttc ttaaggcccg cgggcggcgc
     8401 aggagcggca ctcgtggctg tggtggcttc ggcagcggct tcagcagatc ggcggcatca
     8461 gcggtagcac cagcactagc agcatgagaa aaatgctggc tgccgtgagc cgagtgctgt
     8521 ccggggcaag tcaaaagcca gcctcaagag tcttggtggc aagtagaaat ttcattcatg
     8581 gtaccgtcga cgactacaag gacgacgatg acaaggacta caaagacgat gacgacaagg
     8641 gatccattct caagatcttg aatgagatag cttcaattgg aagtaccaaa caaaagcaag
     8701 caatactgga aaagaataag gataacgaac tgttgaagcg ggtttaccgc ctgacttatt
     8761 cacgggggct tcaatattat attaagaaat ggcctaaacc gggaatcgct actcaatctt
     8821 tcggtatgct cacgcttact gacatgcttg atttcatcga attcacgctg gccacgcgaa
     8881 agttgacagg aaatgcggca atcgaggagc ttaccggcta cataacggac ggaaagaagg
     8941 atgacgtaga ggtccttaga agggtaatga tgagagatct ggagtgcgga gcgtcagtaa
     9001 gcattgccaa caaggtttgg cccggtctga taccagagca accgcaaatg cttgccagta
     9061 gttacgacga aaaggggatt aacaaaaaca ttaaatttcc cgcctttgct caactcaaag
     9121 cagatggagc acgctgtttt gcggaggtcc ggggagatga actggatgat gtcagactcc
     9181 tgagcagagc aggaaacgag taccttggat tggatttgct caaggaagag ttgataaaga
     9241 tgactgcaga agcaaggcaa atacaccctg aaggcgtact tattgacggt gaactggttt
     9301 atcatgagca ggtaaaaaaa gagccagagg gactcgactt tttgtttgac gcttatcctg
     9361 agaacagtaa ggcaaaggag tttgcagaag tagcagagtc ccgaacggca tcaaatggca
     9421 ttgcaaataa aagtctcaaa ggcacaatca gtgaaaaaga agctcagtgt atgaagttcc
     9481 aggtgtggga ttacgtaccg cttgttgaaa tctattctct tccggctttc agactcaagt
     9541 atgatgtgcg cttttctaaa ttggagcaaa tgacgtccgg gtatgacaag gtgattctta
     9601 ttgaaaatca agtggtgaac aatctggatg aagcgaaggt gatttataaa aaatatatag
     9661 accagggttt ggaaggcatt atactgaaga acatcgacgg gttgtgggag aacgcacgct
     9721 ccaaaaacct gtacaagttt aaggaggtga ttgacgtgga tcttaagata gtaggaatat
     9781 atcctcatcg aaaagaccca actaaagctg gagggttcat cttggagagt gaatgcggaa
     9841 agattaaagt gaatgcaggc tcagggctga aggacaaggc gggcgtaaaa tctcacgaat
     9901 tggaccggac tcgaataatg gagaaccaaa attattatat agggaagata cttgaatgtg
     9961 agtgtaatgg atggcttaag tctgatggga ggacagacta cgtaaagctg tttctgccga
    10021 ttgccattag attgcgggaa gacaagacca aggccaatac ttttgaagac gtgttcggtg
    10081 attttcacga ggtgaccggg ctggctagcg ctctgcagaa aaaactcgag gagctggaac
    10141 tggattctag ataaaccacg atcgttatgc tgagtatgtt aagctcttta tgactgtttt
    10201 tgtagtggta tagagtactg cagaatacag taagctgctc tattgtagca tttcttgatg
    10261 ttgcttagtc acttatttca taaacaactt aatgttctga ataatttctt actaaacatt
    10321 ttgttattgg gcaagtgatt gaaaatagta aatgctttgt gtgattgaat cgaattcagg
    10381 gcccgtttaa acggatccct cccccccccc taacgttact ggccgaagcc gcttggaata
    10441 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
    10501 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
    10561 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
    10621 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
    10681 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
    10741 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
    10801 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
    10861 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
    10921 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatgg
    10981 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
    11041 ccatgaacgg ccacgagttc gagatcgagg gcgag
//
