LOCUS       sequence-46778-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ANKRA2
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             614..631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(684..702)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      698..745
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcgcaa attctttgtc tgttcaccag ttggctgctc
      121 agggagagat gctctatctg gctactcgta tcgaacaaga aaatgttatc aatcacacgg
      181 atgaagaagg atttactcct ctgatgtggg ctgcagcaca cgggcaaata gctgtggtag
      241 agttcctact tcagaatggt gctgatcccc aacttttagg aaaaggtcga gaaagtgcac
      301 tgtcgttggc ctgtagtaaa ggctacacag atattgtcaa aatgctgctt gattgtggag
      361 ttgatgtaaa tgaatatgat tggaatggag gaacacctct gctttatgct gtacatggaa
      421 atcatgtgaa atgtgtaaag atgctcttag aaagtggggc tgatccaaca attgaaactg
      481 actctggata taattctatg gatctagctg tagccctagg ctatagaagt gttcaacagg
      541 ttattgagtc acatttgttg aagctgcttc aaaatatcaa ggagtgatga cgaagcttgc
      601 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      661 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      721 taaacgggtc ttgaggggtt ttttgctgaa aggagganct atatccggat tggcgaatgg
      781 gacgcgccct gtagcggcgc attaagcgcg gcggggtgtg gtggttacgc gcagcgtgac
      841 cgctacactt gccagcgccc tagcgcccgc tccnttccgc tttcttccct tccct
//
