LOCUS       sequence-467902-        9183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human IFIT5_K197R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-IFIT5_K197R-VA
FEATURES             Location/Qualifiers
     source          1..9183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2799..2823)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2903..2926
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2963..2986
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2999..3016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3026..3046
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3059..3079
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3092..3157
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3205..3322
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3371..3881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3800..3818
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3903..4502
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3903..3922)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4399..4419
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4630..4863
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4935..5056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4972..4991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5026..5045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5096..5231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5251..5270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5252..5270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5280..5297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5280..5296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5289..5311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5438..5893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5525..5544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5735..5756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5919..6023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6024..6092,6093..6884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6242..6261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7055..7643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7544..7563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7797..7814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7931..7952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7967..7972,7973..7990,7991..7997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8005..8021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8010..8032
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8029..8045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8029..8045
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8064..8084
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8066..8084
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8112..8338
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8339..8519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8566..8691
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcac catgagtgaa attcgtaagg acaccttgaa
     1381 ggccattctg ttggagttag aatgtcattt tacatggaat ttacttaagg aagacattga
     1441 tctgtttgag gtagaagata caattgggca acagcttgaa tttcttacca caaaatctag
     1501 acttgctctt tataacctat tggcctatgt gaaacaccta aaaggccaaa ataaagacgc
     1561 ccttgagtgc ttggaacaag cagaagaaat aatccagcaa gaacactcag acaaagaaga
     1621 agtacgaagc ctggtcactt ggggaaacta tgcctgggtg tattatcaca tggaccagct
     1681 tgaagaagct cagaagtata caggtaagat agggaatgtc tgtaagaaat tgtccagtcc
     1741 ttctaactac aagttggagt gtcctgagac tgactgtgag aaaggctggg cactcttgaa
     1801 atttggagga aagtattatc aaaaggctaa agcggctttt gagaaggctc tggaagtgga
     1861 gcctgacaat ccagaattta acatcggcta tgctatcaca gtgtatcggc tggatgattc
     1921 tgatagagaa gggtctgtac ggagcttttc tctggggcct ttgagaaagg ctgttaccct
     1981 gaacccagat aacagctata ttaaggtttt tctggcactg aagcttcaag atgtacatgc
     2041 agaagctgaa ggggaaaagt atattgaaga aatcctggac caaatatcat cccagcctta
     2101 cgtccttcgt tatgcagcca agttctatag gagaaaaaat tcctggaaca aagctctcga
     2161 acttttaaaa aaggccttgg aggtgacacc aacttcttct ttcctgcatc accagatggg
     2221 actttgctac agggcacaaa tgatccaaat caagaaggcc acacacaaca gacctaaagg
     2281 aaaggataaa ctaaaggttg atgagctgat ttcatctgct atatttcatt tcaaagcagc
     2341 catggaacga gactctatgt ttgcatttgc ctacacagac ctggccaaca tgtatgctga
     2401 aggaggccag tatagcaatg ctgaggacat tttccggaaa gctcttcgtc tggagaacat
     2461 aaccgatgat cacaaacatc agatccatta ccactatggc cgctttcagg aatttcaccg
     2521 taaatcagaa aatactgcca tccatcatta tttagaagcc ttaaaggtca aagacagatc
     2581 accccttcgc accaaactga caagtgctct gaagaaattg tctaccaaga gactttgtca
     2641 caatgcttta gatgtgcaga gtttaagtgc cctagggttt gtttacaagc tggaaggaga
     2701 aaagaggcaa gctgctgagt actatgagaa ggcacaaaag atagatccag aaaatgcaga
     2761 attcctgact gctctctgtg agctccgact ttccattttg ccaactttct tgtacaaagt
     2821 ggttgattct agagcgatcg ccagagagct gttcaacctg ctgggcgaga accagccccc
     2881 cgtggtgatc aaggcgatcg cctggagcca ccctcagttc gagaagggag gaggaagcgg
     2941 cggaggcagc ggaggaggaa gctggagcca cccgcagttc gagaaaggcg gaagcggcca
     3001 tcaccatcac catcaccctg caggagaaaa tctgtacttc caggggagag gaggcagcga
     3061 gaacctgtac tttcaggggc ctgcaggaag agactacaaa gaccatgacg gtgattataa
     3121 agatcatgac atcgattaca aggatgacga tgacaagtag ttcgaattct cgacctcgag
     3181 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3241 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3301 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     3361 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     3421 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     3481 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     3541 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     3601 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     3661 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     3721 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     3781 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     3841 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta
     3901 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     3961 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4021 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4081 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     4141 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     4201 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4261 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     4321 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4381 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4441 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4501 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
     4561 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4621 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4681 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4741 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4801 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4861 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4921 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4981 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5041 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5101 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5161 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5221 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5281 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5341 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5401 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5461 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5521 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5581 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5641 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5701 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5761 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5821 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5881 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6001 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6061 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6121 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6181 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6241 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6301 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6361 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6421 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6481 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6541 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6601 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6661 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6721 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6781 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6841 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7201 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7741 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7801 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7861 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7921 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7981 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8041 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8101 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     8161 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     8221 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     8281 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     8341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8521 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8581 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8641 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8701 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8761 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8821 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8881 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8941 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9001 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9061 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9121 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9181 aat
//
