LOCUS       sequence-468103-       10561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for 
            visualization of the Pcc mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Zhang Z, Xu A, Bai Y, Chen Y, Cates K, Kerr C, Bermudez A, Susanto 
            TT, Wysong K, Garcia Marques FJ, Nolan GP, Pitteri S, Barna M
  TITLE     A subcellular map of translational machinery composition and 
            regulation at the single-molecule level.
  JOURNAL   Science. 2025 Mar 7;387(6738):eadn2623. doi: 
            10.1126/science.adn2623. Epub 2025 Mar 7.
  PUBMED    40048539
REFERENCE   2  (bases 1 to 10561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-03-7"; volume: "387(6738):eadn2623. doi"; pages: " 
            10.1126/science.adn2623. Epub 2025 Mar 7"
COMMENT     Sequence Label: pcDNA4TO_24xGCN4_PCC_24xPP7
FEATURES             Location/Qualifiers
     source          1..10561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(6569..6586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6575..6799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6845..7273
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6932..6951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7142..7163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7282..7302)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7287..7616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7467..7602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7529..7548
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7664..7711
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7730..8104
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8234..8355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8271..8290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8325..8344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8404..8420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8404..8420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8417..8439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8428..8444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8452..8458,8459..8476,8477..8482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8497..8518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8635..8652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8806..9394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8886..8905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9565..10356,10357..10425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10188..10207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10426..10530)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaggc ggggcagtgg gtcaggaccg
     2821 tggcgctgtt ggcggccagg cggcattggc ggcggtcctc gcagcagcaa ttgctgggga
     2881 cgctgaagca tgctccagtc tattcatacc aatgcctagt ggtgtccaga agtctcagtt
     2941 ctgtggaata tgagcctaaa gaaaagactt ttgataaaat tctcattgct aacagaggag
     3001 aaattgcctg tagggttatt aaaacttgca agaagatggg catcaagaca gttgccattc
     3061 acagtgatgt tgatgccagt tctgttcacg tgaaaatggc ggatgaggct gtctgtgttg
     3121 gcccagctcc caccagtaaa agctacctca acatggatgc catcatggaa gccattaaga
     3181 aaaccagggc ccaagctgta cacccagggt atggattcct gtcagaaaac aaagagtttg
     3241 caaagcgtct ggcagcagaa gatgtcactt tcattggacc tgatactcat gctattcaag
     3301 ccatgggtga caagatagaa agcaaactat tagccaagag agcaaaggtc aacacaatcc
     3361 ctggttttga tggggtagta aaggatgcag atgaagctgt cagaattgca agggaaattg
     3421 gctaccctgt gatgatcaag gcctcagcag gcggtggtgg gaaaggcatg cgcatcgcct
     3481 gggatgacga agagaccagg gatggcttta gattttcatc ccaggaagct gcttctagtt
     3541 ttggtgatga tagactacta atagaaaaat ttatcgataa ccctcgtcat atagaaatcc
     3601 aggttttagg ggataaacat ggcaatgctc tgtggctcaa tgagagggag tgctcgatcc
     3661 agagaaggaa ccagaaggtg gtagaggagg cgccaagcat ttttctggat cctgaaactc
     3721 gccaagcaat gggagagcag gccgtggctt tggctaaagc cgtgaagtat tcttctgctg
     3781 gaactgtgga atttcttgtg gactcccaga agaattttta cttcttggag atgaatacaa
     3841 gactacaggt cgaacatcct gtcacagagt gcattactgg cctggactta gtccaagaaa
     3901 tgatccttgt tgctaagggt tacccactca ggcacaagca agaggatatt cccatcagtg
     3961 gctgggcagt tgaatgtcgg gtttatgctg aggaccccta caagtctttc ggtttaccgt
     4021 ctattgggag gctgtcccag taccaagagc cgatacatct acctggtgtc cgagttgaca
     4081 gtggcatcca accaggaagt gacatcagca tctattatga tcctatgatt tcaaagctag
     4141 tcacatatgg gtctgacaga gcagaagccc tgaagaggat ggaagacgca ctggacaatt
     4201 atgtgatccg gggtgttaca cacaacatcc cattgctccg ggaggtgata atcaacacac
     4261 gttttgtgaa aggagacatc agcactaagt ttctctctga tgtgtatcct gatggcttca
     4321 aagggcacac gttaacactg agtgagagaa accagttatt ggccattgca tcatctgtat
     4381 ttgtggcatc ccagctacga gctcagcgct tccaagaaca ttcaagagta ccagttatta
     4441 ggcctgatgt ggctaagtgg gagctctcgg taaagttaca tgatgaagat catactgtcg
     4501 tggcatctaa caatgggccg gcatttaccg tggaagttga tggctcgaaa ctaaatgtga
     4561 ccagtacgtg gaacctggcg tcacccttat tgtctgtcaa cgttgatggc acgcagagga
     4621 ctgtgcagtg tctttctcgg gaagcaggtg gaaacatgag catccagttt cttggcacag
     4681 tgtacaaagt gcacatttta accaagcttg ctgcagagct gaacaaattc atgcttgaaa
     4741 aagtgcccaa ggacaccagc agcactctgt gctccccgat gcctggagtg gtggtggccg
     4801 tttctgtcaa gcctggagac atggtagcag aaggtcagga aatctgtgtg attgaagcta
     4861 tgaaaatgca gaacagtatg acagctggga aaatgggcaa ggtgaaattg gtgcactgca
     4921 aagctggaga cacagttggt gaaggagacc tgcttgtgga gctggaaatc taacgacacg
     4981 attcgaagat cgcatcgcta gcccggttgt acaggtaccg gatcctaagg tacctaattg
     5041 cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag
     5101 agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt
     5161 gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca
     5221 gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat
     5281 tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc
     5341 agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa
     5401 ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag
     5461 cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta
     5521 attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca
     5581 gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct
     5641 aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc
     5701 agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc
     5761 taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac
     5821 cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac
     5881 ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa
     5941 ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta
     6001 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     6061 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     6121 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     6181 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     6241 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     6301 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     6361 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     6421 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatctcgc
     6481 gaagggcgaa ttcacgaggc gcgcccggat cgcgatcgca atcttaatta actcgagtct
     6541 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6601 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6661 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6721 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6781 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6841 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6901 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6961 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7021 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7081 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7141 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7201 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7261 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7321 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7441 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7621 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     7681 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     7741 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     7801 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     7861 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     7921 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     7981 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     8041 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     8101 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     8161 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8221 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8341 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     8401 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8461 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8521 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8581 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9241 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9841 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9901 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9961 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10021 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10081 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10141 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10201 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10261 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10321 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10381 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10441 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10501 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10561 c
//
