LOCUS       sequence-468123-        7868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - gamma-mGFP (gamma-mGFP based)
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(425..443)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(464..480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(477..499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    488..504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(512..518,519..536,537..542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    557..578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(695..712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(866..1454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(946..965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1625..2416,2417..2485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2248..2267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2486..2590)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2617..3072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2704..2723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2914..2935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3198..3220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3212..3229
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3213..3229
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3236..3255
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3236..3254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7152..7868
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ataccgtcga cctcgaggag tgaaattctg gaaatctgga aatctggttt tgtattcttg
       61 ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa atctttgttt
      121 attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta tttgagagat
      181 tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa aaaaacaaaa
      241 caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt ctatatcttg
      301 ttgttgttgt tttcatcttc tgtatcagag gaacttattt tattattttc gtcacgggta
      361 ttttctcttg tttgatgatt catcccattc attccatcaa aaatgtcggt accagctttt
      421 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
      481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      541 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      601 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      661 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2581 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     2641 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2701 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2761 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2821 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2881 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2941 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3001 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3061 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3121 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3181 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     3241 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgcgttt ataataaact
     3301 tagtctaata tttcttttgg accaaaatag aaactgttct caggtggcaa aaaacagctt
     3361 aaagtctgtg ttaaaaaaag atctcggcta tgccaatcaa aaaggagtga cattattatg
     3421 taaaagaaga aatgttgtaa ttccgcgaaa caacaatttt aatgcttact gtgccgccac
     3481 caatatccta tggccaaggg gcctttaaac atgccacgga aatatacagt caaagtaaac
     3541 ctactatgtc tttagagcct tcaggatcaa ttgtttttaa atgaatgact gcaaaactag
     3601 aaaagtagcc tcttacataa gcaagtcatt tgtacaccga gagtttagta accagtgaag
     3661 caagtatatc tggcaaacct gaaacttatc tgtgaattat aaagctcata agtaaaaagt
     3721 gtagaaaaga agataaggca gaccaaatac aaatttgcaa cagtacaacg aatatttcag
     3781 cgtcaaaact agagaataat aaagaaaacg atcttttcaa aaagaaaaaa ccttttagtt
     3841 ttcctttgtt gttgttgtgg gtgtgtgcta tttatattat atagtttact cataatacca
     3901 taaaatattc ggtttgatta ggttatttta ataagctaat ttgtttctaa tcgtgtaatt
     3961 tatgctgtgt atattaagta gtgtgtgcac tgcccaaaaa tgtttgttgt ttatagtcgg
     4021 ttaaagagaa aaaagaaaaa aagatccata cacacacgtt aattagttgt tcaacgtaat
     4081 acactcatat tttgttctta tttgctttcg gtcgctgttc tcaccaagat ttattgccaa
     4141 cgaaacaatt tttttttata tattttcaga tttttctttt tttcctttcc tttccttttc
     4201 taattttcac tcctggtttt ctttctttct tagaaacatt atctcgatat taatattaaa
     4261 aaaatataat cattcaaact ccggaatgtc tactactttg gatgatactg cttatagata
     4321 cagaacttct gttccaggtg atgctgaagc tattgaagct ttggatggtt ctttcactac
     4381 cgatactgtt tttagagtta ctgctactgg tgatggtttc actttgagag aagttccagt
     4441 tgatccacca ttgactaagg ttttcccaga tgatgaatcc gatgatgaat ccgatgctgg
     4501 tgaagatggt gatccagatt ctagaacttt cgttgcttat ggtgatgatg gtgatttggc
     4561 tggtttcgtt gttgtttctt attctggttg gaacagaaga ttgactgttg aagatattga
     4621 agttgctcca gaacatagag gtcatggtgt tggtagagct ttgatgggtt tggctactga
     4681 attcgccaga gaaagaggtg ctggtcattt gtggttggaa gttaccaatg ttaatgctcc
     4741 agctattcat gcttatagaa gaatgggttt cactttgtgt ggtttggata ctgctttata
     4801 cgatggtact gcttccgatg gtgaacaagc tttgtatatg tctatgccat gtccataata
     4861 actcgggggg atccgagtga aattctggaa atctggaaat ctggttttgt attcttgtta
     4921 ttcttctttt tgttattaca tatataactt gttacttttt taaaaaaatc tttgtttatt
     4981 ttataaatat ataaaactaa atttaagaaa aagagaaaaa tgttttattt gagagattga
     5041 aattttactt gaatttagct tagcttttat aaagtattat tatgtaaaaa aacaaaacaa
     5101 atatacatta aaaagttaag actataaaat agccacccaa ggcatttcta tatcttgttg
     5161 ttgttgtttt catcttctgt atcagaggaa cttattttat tattttcgtc acgggtattt
     5221 tctcttgttt gatgattcat cccattcttc catcataaaa tactagtgga tcccccagat
     5281 ccttatcctt aaacagattc taaaacaaca tctttgaaac cagaagaaac tttcttacct
     5341 ttttcttcgg ttgaaccttc accgtgcaaa gccaataatg aacccaagtc gaattttggt
     5401 tgtttcaaca atttgacttt tctgatgtgg acattttgta atgggaaaat ggtttgggtg
     5461 gatttttcaa tttcacggcc aatgacttct ggaatcaatt tggaagttaa ttgagctaaa
     5521 gtacagttgg aaacttctct ttgcataatt tcaatcatct tctttctgac ttctctcaat
     5581 ttagaggatt gagcgtaagt agttttcttg atttggtttg gttgtctttt ggtgaaagca
     5641 atggcaaaaa ctctcaaaac gtaatcgtcg gaagttttaa cagtgacatt agcttcaact
     5701 aatgattgcc attttctgac caatgatctt aatttgtcag aagtgaaatc caaaccatga
     5761 ggcctccatg aggcctccat gaggcctcca tgaggcctcc atggaggcct catggaagtt
     5821 ggtcaacaag tttttacctt ggacttcatc aactctcaat ttgatttttc tgtaagagtg
     5881 gtcttcggaa ccttgtaagt cggccaaaca aacttcgaaa actctacctt tcaagccatc
     5941 agcggcattc tttaaaccgg tagatctgtt gatcaaagtt ttaccaacat ttctgttttc
     6001 aaaagtggtt ggagctttga tgtcaaacca atcttttctg gtgaatgggt caacgacctt
     6061 cttttttaat cctttctttc ctttggacaa tctcttgttt ttaccgacag ccatgttgta
     6121 cttgagttgg atctacgcgt cagcctcgtt tataataaac ttagtctaat atttcttttg
     6181 gaccaaaata gaaactgttc tcaggtggca aaaaacagct taaagtctgt gttaaaaaaa
     6241 gatctcggct atgccaatca aaaaggagtg acattattat gtaaaagaag aaatgttgta
     6301 attccgcgaa acaacaattt taatgcttac tgtgccgcca ccaatatcct atggccaagg
     6361 ggcctttaaa catgccacgg aaatatacag tcaaagtaaa cctactatgt ctttagagcc
     6421 ttcaggatca attgttttta aatgaatgac tgcaaaacta gaaaagtagc ctcttacata
     6481 agcaagtcat ttgtacaccg agagtttagt aaccagtgaa gcaagtatat ctggcaaacc
     6541 tgaaacttat ctgtgaatta taaagctcat aagtaaaaag tgtagaaaag aagataaggc
     6601 agaccaaata caaatttgca acagtacaac gaatatttca gcgtcaaaac tagagaataa
     6661 taaagaaaac gatcttttca aaaagaaaaa accttttagt tttcctttgt tgttgttgtg
     6721 ggtgtgtgct atttatatta tatagtttac tcataatacc ataaaatatt cggtttgatt
     6781 aggttatttt aataagctaa tttgtttcta atcgtgtaat ttatgctgtg tatattaagt
     6841 agtgtgtgca ctgcccaaaa atgtttgttg tttatagtcg gttaaagaga aaaaagaaaa
     6901 aaagatccat acacacacgt taattagttg ttcaacgtaa tacactcata ttttgttctt
     6961 atttgctttc ggtcgctgtt ctcaccaaga tttattgcca acgaaacaat ttttttttat
     7021 atattttcag atttttcttt ttttcctttc ctttcctttt ctaattttca ctcctggttt
     7081 tctttctttc ttagaaacat tatctcgata ttaatattaa aaaaatataa tcattcaaaa
     7141 tgctgcaggc catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg
     7201 aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg
     7261 ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat
     7321 ggccaacctt agtcactact ttatgttatg gtgttcaatg tttttcaaga tacccagatc
     7381 atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa
     7441 ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg
     7501 ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt
     7561 taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac
     7621 aaaagaatgg tatcaaagct aacttcaaaa ctagacacaa cattgaagat ggttctgttc
     7681 aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag
     7741 acaaccatta cttatccact caatctaaat tatccaaaga tccaaacgaa aagagagacc
     7801 acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt
     7861 acaaataa
//
