LOCUS       sequence-468237-       15928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low copy number provides expression of human DNA-PKcs with the 6 
            ABCDE phosphorylation sites substituted to alanine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, 
            Chaplin AK, Meek K
  TITLE     Two distinct long-range synaptic complexes promote different aspects
            of end processing prior to repair of DNA breaks by non-homologous 
            end joining.
  JOURNAL   Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 
            10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31.
  PUBMED    36724784
REFERENCE   2  (bases 1 to 15928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.012"; journalName: "Mol Cell"; date: 
            "2023-03-2- 2"; volume: "83"; issue: "5"; pages: "698-714.e4"
COMMENT     Sequence Label: RMCE ABCDE>ala DNA-PKcs
FEATURES             Location/Qualifiers
     source          1..15928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(210..278,279..1070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(428..447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2065..2087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2084..2100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2134..2167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2315..2618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(15502..15519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15508..15732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(15770..15803)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(15836..15852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15885..15901)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15894..15916)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcaagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg ccaagcttgc atgcctgcag gggataactt cgtataatgt atgctatacg
     2161 aagttatggg gccgctctag cctcgaggcc ggccgtaatg agacgcacaa actaatatca
     2221 caaactggaa atgtctatca atatatagtt gctctagtta ttaatagtaa tcaattacgg
     2281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2581 ggcagtacat ctacgtatta gtcatcgcta ttaccatgca tggtcgaggt gagccccacg
     2641 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     2701 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc ccgggggggg
     2761 gggggggggg gggggggggg gggggggggg ggggggggcg agggggaggg gtgcggcggc
     2821 ggccaatcgg agcggcgcgc tccgaaagtt tccttttatg gcgggggggc ggcggcgggg
     2881 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     2941 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     3001 acaggtgagc gggcgggacg gcccttctcc ttcgggctgt aattagcatc acgcgtggtt
     3061 tttccgctag ccatggcggg ctccggagcc ggtgtgcgtt gctccctgct gcggctgcag
     3121 gagaccttgt ccgctgcgga ccgctgcggt gctgccctgg ccggtcatca actgatccgc
     3181 ggcctggggc aggaatgcgt cctgagcagc agccccgcgg tgctggcatt acagacatct
     3241 ttagtttttt ccagagattt cggtttgctt gtatttgtcc ggaagtcact caacagtatt
     3301 gaatttcgtg aatgtagaga agaaatccta aagtttttat gtattttctt agaaaaaatg
     3361 ggccagaaga tcgcacctta ctctgttgaa attaagaaca cttgtaccag tgtttataca
     3421 aaagatagag ctgctaaatg taaaattcca gccctggacc ttcttattaa gttacttcag
     3481 acttttagaa gttctagact catggatgaa tttaaaattg gagaattatt tagtaaattc
     3541 tatggagaac ttgcattgaa aaaaaaaata ccagatacag ttttagaaaa agtatatgag
     3601 ctcctaggat tattgggtga agttcatcct agtgagatga taaataatgc agaaaacctg
     3661 ttccgcgctt ttctgggtga acttaagacc cagatgacat cagcagtaag agagcccaaa
     3721 ctacctgttc tggcaggatg tctgaagggg ttgtcctcac ttctgtgcaa cttcactaag
     3781 tccatggaag aagatcccca gacttcaagg gagattttta attttgtact aaaggcaatt
     3841 cgtcctcaga ttgatctgaa gagatatgct gtgccctcag ctggcttgcg cctatttgcc
     3901 ctgcatgcat ctcagtttag cacctgcctt ctggacaact acgtgtctct atttgaagtc
     3961 ttgttaaagt ggtgtgccca cacaaatgta gaattgaaaa aagctgcact ttcagccctg
     4021 gaatcctttc tgaaacaggt ttctaatatg gtggcgaaaa atgcagaaat gcataaaaat
     4081 aaactgcagt actttatgga gcagttttat ggaatcatca gaaatgtgga ttcgaacaac
     4141 aaggagttat ctattgctat ccgtggatat ggactttttg caggaccgtg caaggttata
     4201 aacgcaaaag atgttgactt catgtacgtt gagctcattc agcgctgcaa gcagatgttc
     4261 ctcacccaga cagacactgg tgacgaccgt gtttatcaga tgccaagctt cctccagtct
     4321 gttgcaagcg tcttgctgta ccttgacaca gttcctgagg tgtatactcc agttctggag
     4381 cacctcgtgg tgatgcagat agacagtttc ccacagtaca gtccaaaaat gcagctggtg
     4441 tgttgcagag ccatagtgaa ggtgttccta gctttggcag caaaagggcc agttctcagg
     4501 aattgcatta gtactgtggt gcatcagggt ttaatcagaa tatgttctaa accagtggtc
     4561 cttccaaagg gccctgagtc tgaatctgaa gaccaccgtg cttcagggga agtcagaact
     4621 ggcaaatgga aggtgcccac atacaaagac tacgtggatc tcttcagaca tctcctgagc
     4681 tctgaccaga tgatggattc tattttagca gatgaagcat ttttctctgt gaattcctcc
     4741 agtgaaagtc tgaatcattt actttatgat gaatttgtaa aatccgtttt gaagattgtt
     4801 gagaaattgg atcttacact tgaaatacag actgttgggg aacaagagaa tggagatgag
     4861 gcgcctggtg tttggatgat cccaacttca gatccagcgg ctaacttgca tccagctaaa
     4921 cctaaagatt tttcggcttt cattaacctg gtggaatttt gcagagagat tctccctgag
     4981 aaacaagcag aattttttga accatgggtg tactcatttt catatgaatt aattttgcaa
     5041 tctacaaggt tgcccctcat cagtggtttc tacaaattgc tttctattac agtaagaaat
     5101 gccaagaaaa taaaatattt cgagggagtt agtccaaaga gtctgaaaca ctctcctgaa
     5161 gacccagaaa agtattcttg ctttgcttta tttgtgaaat ttggcaaaga ggtggcagtt
     5221 aaaatgaagc agtacaaaga tgaacttttg gcctcttgtt tgacctttct tctgtccttg
     5281 ccacacaaca tcattgaact cgatgttaga gcctacgttc ctgcactgca gatggctttc
     5341 aaactgggcc tgagctatac ccccttggca gaagtaggcc tgaatgctct agaagaatgg
     5401 tcaatttata ttgacagaca tgtaatgcag ccttattaca aagacattct cccctgcctg
     5461 gatggatacc tgaagacttc agccttgtca gatgagacca agaataactg ggaagtgtca
     5521 gctctttctc gggctgccca gaaaggattt aataaagtgg tgttaaagca tctgaagaag
     5581 acaaagaacc tttcatcaaa cgaagcaata tccttagaag aaataagaat tagagtagta
     5641 caaatgcttg gatctctagg aggacaaata aacaaaaatc ttctgacagt cacgtcctca
     5701 gatgagatga tgaagagcta tgtggcctgg gacagagaga agcggctgag ctttgcagtg
     5761 ccctttagag agatgaaacc tgtcattttc ctggatgtgt tcctgcctcg agtcacagaa
     5821 ttagcgctca cagccagtga cagacaaact aaggttgcag cctgtgaact tttacatagc
     5881 atggttatgt ttatgttggg caaagccacg cagatgccag aagggggaca gggagcccca
     5941 cccatgtacc agctctataa gcggacgttt cctgtgctgc ttcgacttgc gtgtgatgtt
     6001 gatcaggtga caaggcaact gtatgagcca ctagttatgc agctgattca ctggttcact
     6061 aacaacaaga aatttgaaag tcaggatact gttgccttac tagaagctat attggatgga
     6121 attgtggacc ctgttgacag tactttaaga gatttttgtg gtcggtgtat tcgagaattc
     6181 cttaaatggt ccattaagca aataacacca cagcagcagg agaagagtcc agtaaacacc
     6241 aaatcgcttt tcaagcgact ttatagcctt gcgcttcacc ccaatgcttt caagaggctg
     6301 ggagcatcac ttgcctttaa taatatctac agggaattca gggaagaaga gtctctggtg
     6361 gaacagtttg tgtttgaagc cttggtgata tacatggaga gtctggcctt agcacatgca
     6421 gatgagaagt ccttaggtac aattcaacag tgttgtgatg ccattgatca cctatgccgc
     6481 atcattgaaa agaagcatgt ttctttaaat aaagcaaaga aacgacgttt gccgcgagga
     6541 tttccacctt ccgcatcatt gtgtttattg gatctggtca agtggctttt agctcattgt
     6601 gggaggcccc agacagaatg tcgacacaaa tccattgaac tcttttataa attcgttcct
     6661 ttattgccag gcaacagatc ccctaatttg tggctgaaag atgttctcaa ggaagaaggt
     6721 gtctcttttc tcatcaacac ctttgagggg ggtggctgtg gccagccctc gggcatcctg
     6781 gcccagccca ccctcttgta ccttcggggg ccattcagcc tgcaggccac gctatgctgg
     6841 ctggacctgc tcctggccgc gttggagtgc tacaacacgt tcattggcga gagaactgta
     6901 ggagcgctcc aggtcctagg tactgaagcc cagtcttcac ttttgaaagc agtggctttc
     6961 ttcttagaaa gcattgccat gcatgacatt atagcagcag aaaagtgctt tggcactggg
     7021 gcagcaggta acagaacaag cccacaagag ggagaaaggt acaactacag caaatgcacc
     7081 gttgtggtcc ggattatgga gtttaccacg actctgctaa acacctcccc ggaaggatgg
     7141 aagctcctga agaaggactt gtgtaataca cacctgatga gagtcctggt gcagacgctg
     7201 tgtgagcccg caagcatagg tttcaacatc ggagacgtcc aggttatggc tcatcttcct
     7261 gatgtttgtg tgaatctgat gaaagctcta aagatgtccc catacaaaga tatcctagag
     7321 acccatctga gagagaaaat aacagcacag agcattgagg agctttgtgc cgtcaacttg
     7381 tatggccctg acgcgcaagt ggacaggagc aggctggctg ctgttgtgtc tgcctgtaaa
     7441 cagcttcaca gagctgggct tctgcataat atattaccgt ctcagtccac agatttgcat
     7501 cattctgttg gcacagaact tctttccctg gtttataaag gcattgcccc tggagatgag
     7561 agacagtgtc tgccttctct agacctcagt tgtaagcagc tggccagcgg acttctggag
     7621 ttagcctttg cttttggagg actgtgtgag cgccttgtga gtcttctcct gaacccagcg
     7681 gtgctgtcca cggcgtcctt gggcagctca cagggcagcg tcatccactt ctcccatggg
     7741 gagtatttct atagcttgtt ctcagaaacg atcaacacgg aattattgaa aaatctggat
     7801 cttgctgtat tggagctcat gcagtcttca gtggataata ccaaaatggt gagtgccgtt
     7861 ttgaacggca tgttagacca gagcttcagg gagcgagcaa accagaaaca ccaaggactg
     7921 aaacttgcga ctacaattct gcaacactgg aagaagtgtg attcatggtg ggccaaagat
     7981 tcccctctcg aaactaaaat ggcagtgctg gccttactgg caaaaatttt acagattgat
     8041 tcatctgtat cttttaatac aagtcatggt tcattccctg aagtctttac aacatatatt
     8101 agtctacttg ctgacacaaa gctggatcta catttaaagg gccaagctgt cactcttctt
     8161 ccattcttca ccagcctcac tggaggcagt ctggaggaac ttagacgtgt tctggagcag
     8221 ctcatcgttg ctcacttccc catgcagtcc agggaatttc ctccaggaac tccgcggttc
     8281 aataattatg tggactgcat gaaaaagttt ctagatgcat tggaattatc tcaaagccct
     8341 atgttgttgg aattgatgac agaagttctt tgtcgggaac agcagcatgt catggaagaa
     8401 ttatttcaat ccagtttcag gaggattgcc agaaggggtt catgtgtcac acaagtaggc
     8461 cttctggaaa gcgtgtatga aatgttcagg aaggatgacc cccgcctaag tttcacacgc
     8521 cagtcctttg tggaccgctc cctcctcact ctgctgtggc actgtagcct ggatgctttg
     8581 agagaattct tcagcacaat tgtggtggat gccattgatg tgttgaagtc caggtttaca
     8641 aagctaaatg aatctacctt tgatactcaa atcaccaaga agatgggcta ctataagatt
     8701 ctagacgtga tgtattctcg ccttcccaaa gatgatgttc atgctaagga atcaaaaatt
     8761 aatcaagttt tccatggctc gtgtattaca gaaggaaatg aacttacaaa gacattgatt
     8821 aaattgtgct acgatgcatt tacagagaac atggcaggag agaatcagct gctggagagg
     8881 agaagacttt accattgtgc agcatacaac tgcgccatat ctgtcatctg ctgtgtcttc
     8941 aatgagttaa aattttacca aggttttctg tttagtgaaa aaccagaaaa gaacttgctt
     9001 atttttgaaa atctgatcga cctgaagcgc cgctataatt ttcctgtaga agttgaggtt
     9061 cctatggaaa gaaagaaaaa gtacattgaa attaggaaag aagccagaga agcagcaaat
     9121 ggggattcag atggtccttc ctatatgtct tccctgtcat atttggcaga cagtaccctg
     9181 agtgaggaaa tgagtcaatt tgatttctca accggagttc agagctattc atacagctcc
     9241 caagacccta gacctgccac tggtcgtttt cggagacggg agcagcggga ccccacggtg
     9301 catgatgatg tgctggagct ggagatggac gagctcaatc ggcatgagtg catggcgccc
     9361 ctgacggccc tggtcaagca catgcacaga agcctgggcc cgcctcaagg agaagaggat
     9421 tcagtgccaa gagatcttcc ttcttggatg aaattcctcc atggcaaact gggaaatcca
     9481 atagtaccat taaatatccg tctcttctta gccaagcttg ttattaatac agaagaggtc
     9541 tttcgccctt acgcgaagca ctggcttagc cccttgctgc agctggctgc ttctgaaaac
     9601 aatggaggag aaggaattca ctacatggtg gttgagatag tggccactat tctttcatgg
     9661 acaggcttgg ccactccaac aggggtccct aaagatgaag tgttagcaaa tcgattgctt
     9721 aatttcctaa tgaaacatgt ctttcatcca aaaagagctg tgtttagaca caaccttgaa
     9781 attataaaga cccttgtcga gtgctggaag gattgtttat ccatccctta taggttaata
     9841 tttgaaaagt tttccggtaa agatcctaat tctaaagaca actcagtagg gattcaattg
     9901 ctaggcatcg tgatggccaa tgacctgcct ccctatgacc cacagtgtgg catccagagt
     9961 agcgaatact tccaggcttt ggtgaataat atgtcctttg taagatataa agaagtgtat
    10021 gccgctgcag cagaagttct aggacttata cttcgatatg ttatagagag aaaaaacata
    10081 ctggaggagt ctctgtgtga actggttgcg aaacaattga agcaacatca gaatactatg
    10141 gaggacaagt ttattgtgtg cttgaacaaa gtgaccaaga gcttccctcc tcttgcagac
    10201 aggttcatga atgctgtgtt ctttctgctg ccaaaatttc atggagtgtt gaaaacactc
    10261 tgtctggagg tggtactttg tcgtgtggag ggaatgacag agctgtactt ccagttaaag
    10321 agcaaggact tcgttcaagt catgagacat agagatgatg aaagacaaaa agtatgtttg
    10381 gacataattt ataagatgat gccaaagtta aaaccagtag aactccgaga acttctgaac
    10441 cccgttgtgg aattcgtttc ccatccttct acaacatgta gggaacaaat gtataatatt
    10501 ctcatgtgga ttcatgataa ttacagagat ccagaaagtg agacagataa tgactcccag
    10561 gaaatattta agttggcaaa agatgtgctg attcaaggat tgatcgatga gaaccctgga
    10621 cttcaattaa ttattcgaaa tttctggagc catgaaacta ggttaccttc aaataccttg
    10681 gaccggttgc tggcactaaa ttccttatat tctcctaaga tagaagtgca ctttttaagt
    10741 ttagcaacaa attttctgct cgaaatgacc agcatgagcc cagattatcc aaaccccatg
    10801 ttcgagcatc ctctgtcaga atgcgaattt caggaatata ccattgattc tgattggcgt
    10861 ttccgaagta ctgttctcac tccgatgttt gtggaggctc aggccgctca gggcactctc
    10921 cagacccgtg ctcaggaagg ggctctctca gctcgctggc cagtggcagg gcagataagg
    10981 gccgctcagc agcagcatga cttcacactg gcccagactg cagatggaag aagctcattt
    11041 gattggctga ccgggagcag cactgacccg ctggtcgacc acaccagtcc ctcatctgac
    11101 tccttgctgt ttgcccacaa gaggagtgaa aggttacaga gagcaccctt gaagtcagtg
    11161 gggcctgatt ttgggaaaaa aaggctgggc cttccagggg acgaggtgga taacaaagtg
    11221 aaaggtgcgg ccggccggac ggacctacta cgactgcgca gacggtttat gagggaccag
    11281 gagaagctca gtttgatgta tgccagaaaa ggcgttgctg agcaaaaacg agagaaggaa
    11341 atcaagagtg agttaaaaat gaagcaggat gcccaggtcg ttctgtacag aagctaccgg
    11401 cacggagacc ttcctgacat tcagatcaag cacagcagcc tcatcacccc gttacaggcc
    11461 gtggcccaga gggacccaat aattgcaaaa cagctcttta gcagcttgtt ttctggaatt
    11521 ttgaaagaga tggataaatt taagacactg tctgaaaaaa acaacatcac tcaaaagttg
    11581 cttcaagact tcaatcgttt tcttaatacc accttctctt tctttccacc ctttgtctct
    11641 tgtattcagg acattagctg tcagcacgca gccctgctga gcctcgaccc agcggctgtt
    11701 agcgctggtt gcctggccag cctacagcag cccgtgggca tccgcctgct agaggaggct
    11761 ctgctctgcc tgctgcctgc tgagctgccc gccaagcgag tccgtgggaa ggcccgcccc
    11821 cctcctgatg tcctcagatg ggtggagctt gctaagctgt atagatcaat tggagaatac
    11881 gacgtcctcc gtgggatttt taccagtgag ataggaacaa agcaaatcac tcagagtgca
    11941 ttattagcag aagccagaag tgattattct gaagctgcta agcagtatga tgaggctctc
    12001 aataaacaag actgggtaga tggtgagccc acagaagccg agaaggattt ttgggaactt
    12061 gcatcccttg actgttacaa ccaccttgct gagtggaaat cacttgaata ctgttctaca
    12121 gccagtatag acagtgagaa ccccccagac ctaaataaaa tctggagtga accattttat
    12181 caggaaacat atctacctta catgatccgc agcaagctga agctgctgct ccagggagag
    12241 gctgaccagt ccctgctgac atttattgac aaagctatgc acggggagct ccagaaggcg
    12301 attctagagc ttcattacag tcaagagctg agtctgcttt acctcctgca agatgatgtt
    12361 gacagagcca aatattacat tcaaaatggc attcagagtt ttatgcagaa ttattctagt
    12421 attgatgtcc tcttacacca aagtagactc accaaattgc agtctgtaca ggctttaaca
    12481 gaaattcagg agttcatcag ctttataagc aaacaaggca atttatcatc tcaagttccc
    12541 cttaagagac ttctgaacac ctgggcaaac agatatccag atgctaaaat ggacccaatg
    12601 aacatctggg atgacatcat cacaaatcga tgtttctttc tcagcaaaat agaggagaag
    12661 cttacccctc ttccagaaga taatagtatg aatgtggatc aagatggaga ccccagtgac
    12721 aggatggaag tgcaagagca ggaagaagat atcagctccc tgatcaggag ttgcaagttt
    12781 tccatgaaaa tgaagatgat agacagtgcc cggaagcaga acaatttctc acttgctatg
    12841 aaactactga aggagctgca taaagagtca aaaaccagag acgattggct ggtgagctgg
    12901 gtgcagagct actgccgcct gagccactgc cggagccggt cccagggctg ctctgagcag
    12961 gtgctcactg tgctgaaaac agtctctttg ttggatgaga acaacgtgtc aagctactta
    13021 agcaaaaata ttctggcttt ccgtgaccag aacattctct tgggtacaac ttacaggatc
    13081 atagcgaatg ctctcagcag tgagccagcc tgccttgctg aaatcgagga ggacaaggct
    13141 agaagaatct tagagctttc tggatccagt tcagaggatt cagagaaggt gatcgcgggt
    13201 ctgtaccaga gagcattcca gcacctctct gaggctgtgc aggcggctga ggaggaggcc
    13261 cagcctccct cctggagctg tgggcctgca gctggggtga ttgatgctta catgacgctg
    13321 gcagatttct gtgaccaaca gctgcgcaag gaggaagaga atgcatcagt tactgattct
    13381 gcagaactgc aggcgtatcc agcacttgtg gtggagaaaa tgttgaaagc tttaaaatta
    13441 aattccaatg aagccagatt gaagtttcct agattacttc agattataga acggtatcca
    13501 gaggagactt tgagcctcat gacaaaagag atctcttccg ttccctgctg gcagttcatc
    13561 agctggatca gccacatggt ggccttactg gacaaagacc aagccgttgc tgttcagcac
    13621 tctgtggaag aaatcactga taactacccg caggctattg tttatccctt catcataagc
    13681 agcgaaagct attccttcaa ggatacttct actggtcata agaataagga gtttgtggca
    13741 aggattaaaa gtaagttgga tcaaggagga gtgattcaag attttattaa tgccttagat
    13801 cagctctcta atcctgaact gctctttaag gattggagca atgatgtaag agctgaacta
    13861 gcaaaaaccc ctgtaaataa aaaaaacatt gaaaaaatgt atgaaagaat gtatgcagcc
    13921 ttgggtgacc caaaggctcc aggcctgggg gcctttagaa ggaagtttat tcagactttt
    13981 ggaaaagaat ttgataaaca ttttgggaaa ggaggttcta aactactgag aatgaagctc
    14041 agtgacttca acgacattac caacatgcta cttttaaaaa tgaacaaaga ctcaaagccc
    14101 cctgggaatc tgaaagaatg ttcaccctgg atgagcgact tcaaagtgga gttcctgaga
    14161 aatgagctgg agattcccgg tcagtatgac ggtaggggaa agccattgcc agagtaccac
    14221 gtgcgaatcg ccgggtttga tgagcgggtg acagtcatgg cgtctctgcg aaggcccaag
    14281 cgcatcatca tccgtggcca tgacgagagg gaacaccctt tcctggtgaa gggtggcgag
    14341 gacctgcggc aggaccagcg cgtggagcag ctcttccagg tcatgaatgg gatcctggcc
    14401 caagactccg cctgcagcca gagggccctg cagctgagga cctatagcgt tgtgcccatg
    14461 acctccaggt taggattaat tgagtggctt gaaaatactg ttaccttgaa ggaccttctt
    14521 ttgaacacca tgtcccaaga ggagaaggcg gcttacctga gtgatcccag ggcaccgccg
    14581 tgtgaatata aagattggct gacaaaaatg tcaggaaaac atgatgttgg agcttacatg
    14641 ctaatgtata agggcgctaa tcgtactgaa acagtcacgt cttttagaaa acgagaaagt
    14701 aaagtgcctg ctgatctctt aaagcgggcc ttcgtgagga tgagtacaag ccctgaggct
    14761 ttcctggcgc tccgctccca cttcgccagc tctcacgctc tgatatgcat cagccactgg
    14821 atcctcggga ttggagacag acatctgaac aactttatgg tggccatgga gactggtggc
    14881 gtgatcggga tcgactttgg gcatgcgttt ggatccgcta cacagtttct gccagtccct
    14941 gagttgatgc cttttcggct aactcgccag tttatcaatc tgatgttacc aatgaaagaa
    15001 acgggcctta tgtacagcat catggtacac gcactccggg ccttccgctc agaccctggc
    15061 ctgctcacca acaccatgga tgtgtttgtc aaggagccct cctttgattg gaaaaatttt
    15121 gaacagaaaa tgctgaaaaa aggagggtca tggattcaag aaataaatgt tgctgaaaaa
    15181 aattggtacc cccgacagaa aatatgttac gctaagagaa agttagcagg tgccaatcca
    15241 gcagtcatta cttgtgatga gctactcctg ggtcatgaga aggcccctgc cttcagagac
    15301 tatgtggctg tggcacgagg aagcaaagat cacaacattc gtgcccaaga accagagagt
    15361 gggctttcag aagagactca agtgaagtgc ctgatggacc aggcaacaga ccccaacatc
    15421 cttggcagaa cctgggaagg atgggagccc tggatgtgag gtctgcggcc gcgatctgtc
    15481 gacgataaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    15541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    15601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    15661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    15721 tgggctctat ggcttctgag gcggaaagaa ccagatcgga tccatcgata taacttcgta
    15781 taggagactt tatacgaagt tatctcgatg aaggggttct ctagatcgag aattcactgg
    15841 ccgtcgtttt acaaggcgcg cccccgggta ccgagctcga attcactggc cgtcgtttta
    15901 caacgtcgtg actgggaaaa ccctggcg
//
