LOCUS       sequence-468318-        2073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2073)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV13
FEATURES             Location/Qualifiers
     source          1..2073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(403..419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(403..419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      458..489
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        490..592
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             593..1252
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(659..678)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1276..1303
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1315..1902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1395..1414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1924..1953)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2026..2043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2027..2043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgcttagtcc tgacctgcct gactctgcct ggaacaccag gctcctctgc
       61 catgtcatgc tttgtctcct gggagcagtt tcagtggctg ctggagtcat ccagtcccca
      121 agacatctga tcaaagaaaa gagggaaaca gccactctga aatgctatcc tatccctaga
      181 cacgacactg tttactggta ccagcagggt ccaggtcagg acccccagtt cctcatttcg
      241 ttttatgaaa agatgcagag cgataaagga agcatccctg atcgattctc agctcaacag
      301 ttcagtgact atcattctga actgaacatg agctccttgg agctggggga ctcagccctg
      361 tacttctgtg tgagacgcag ggaagatggc cgcgaagaaa acgtcatagc tgtttcctga
      421 ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg
      481 cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
      541 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
      601 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      661 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      721 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      781 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
      841 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      901 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      961 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1021 ttttgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1081 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1141 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1201 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatgagggcc
     1261 caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca
     1321 taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa
     1381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1741 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga
     1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc
     1921 taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt ccagttacgc
     1981 tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtttgtaa aacgacggcc
     2041 agtgctaacc gtatcgcatt ggccatacct aca
//
