LOCUS       sequence-468505-       10884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pCST:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..93
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      181..891
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1186..1977
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2318..2906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2807..2826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3242..4390
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4536..4560
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5062..5407
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5350..5370
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5498..6292
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5552..5571)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6162..6181
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6305..6557
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      join(10635..10884,1..3)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 atcgacgcta ctagaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta
       61 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
      121 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc
      181 cgcctttgcg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
      241 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg
      301 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
      361 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
      421 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
      481 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
      541 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
      601 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
      661 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
      721 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
      781 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
      841 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcca
      901 attcccgagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      961 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     1021 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     1081 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     1141 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     1201 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1261 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1321 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1381 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1441 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1501 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1561 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1621 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1681 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1741 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1801 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1861 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1921 ttggaagaat ttgtccatta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     1981 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     2041 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     2101 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgctg tcagaccaag
     2161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2941 cagatcctag atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg
     3001 catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct
     3061 ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta
     3121 cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc
     3181 aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg
     3241 aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc
     3301 cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt
     3361 ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca
     3421 actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga
     3481 acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac
     3541 caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc
     3601 cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat
     3661 tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt
     3721 caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt
     3781 caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact
     3841 ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt
     3901 cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa
     3961 ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt
     4021 tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa
     4081 aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca
     4141 ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt
     4201 cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat
     4261 gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga
     4321 agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg
     4381 caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc ctgctcggat
     4441 ctgttggacc ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg
     4501 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     4561 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggttga
     4621 acactctgtg ggtctcatgc cgaattcgga tccggaggtc aacatggtgg agcacgacac
     4681 tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac
     4741 ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca
     4801 cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa
     4861 aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc
     4921 cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg
     4981 atgtgataac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
     5041 ctcagaagat caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct
     5101 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
     5161 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga
     5221 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagaggttcc
     5281 aaccacgtct acaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
     5341 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     5401 gaggacacgc tcgagtataa gagctcattt ttacaacaat taccaacaac aacaaacaac
     5461 aaacaacatt acaattacat ttacaattat cgatacaatg attgaacaag atggattgca
     5521 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5581 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5641 tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa gacgaggcag cgcggctatc
     5701 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5761 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5821 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5881 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5941 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6001 cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg tcgtgaccca
     6061 cggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6121 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6181 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6241 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcttgtca
     6301 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     6361 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     6421 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     6481 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6541 atctatgtta ctagatcgac gctgcaagaa ttcaagcttg gagggagccg tcgcttttgc
     6601 acacagacgg ccgtgctcgt aagcagccca gacacgcaaa cacgcctagt tgcagcccgg
     6661 gagcgcagcc gggctgctgg gccagcgtcg caaacacgcc ctaaatcaga gagggaaagc
     6721 cccacatata taactagatg tcttattcaa atatcacctc taaggattac ttatcctggt
     6781 aaatgagacg attccaggaa gatttgggtt tccaaactgt cccttagaag ggccactcta
     6841 ctgtcagcca ctcttgtcta ggctacttta tactggatac aaactcatta cgctgaagcc
     6901 ctttatattg gtcaatatca tgagatctcc agacgccgcc gtcactcgat cgagatagat
     6961 atagcctctt tccgtccacg tacatccgct gctggccttc agatctctat caagtgacgc
     7021 ggcaaacagg acgcagcacc ggggaactac gacgagtact atatagcaga taaacgcatg
     7081 ctcttggccg ttgttagagc ccttgattgc atggcagtgt cgatcgccca actccagcct
     7141 gagttctcag aacctccccg gccggcccag tgtccagcgt gctgctcaca atctacggga
     7201 tcaggcgatc agctatagta gcccttcaca tcggggtgca tttatgtgtc ttaatttgct
     7261 cagcagtcaa ccatccatca tgcacttgca cttgttcatc gccatggttg gcagggaacc
     7321 aatattaggc ccggaatttc tctttcgtcg atggcccagc tcttttttcc agtgattcat
     7381 tttcacgcac gcctcagaga taaatcttgt gtttattcac tttgtcaatt tggtttgatc
     7441 aaaattacac ttggatttac atcgatatca ttgttttgtg cgatagttgc ttaagtaacc
     7501 atatagaagc tcacatttgt aattaagctg tattcatttc cctagtgtga ttaagtagtt
     7561 gctagattgt gtgcttgagt aaagtcaacc tagagattta gcagctagat ttgttcatgc
     7621 tgtttagtct aatttctttg taaatggaag gtgtactgta cacacttggt gatctaatgt
     7681 gtgcatgctg tttaatctga ctagtatatg atgagcaatg tctatagtag tatatgcttg
     7741 tttatgttac ataatttggc taatatatca gcaatactca tgctaatact tattgtgttc
     7801 attattatta ttattattat tatttcaatc taactaatat atgagcagtg ttcgtacctc
     7861 ggaccaatac ttgagtttgt catgtttcat attctgattt tacccacttt tgaaaaaaca
     7921 acaacccctg ccaccgcttt agtgctagct gtgacaaaca cacgaatgtg aatacatggt
     7981 cagtatacct tctatgcatg gtctatgcat ggtcgaggtg gtggaccagg acgcatcctg
     8041 atgccaaact ctgacctctg tggtgacaaa tgagaaaacc ggcgtctcat ctgatggtgc
     8101 tggaagccgc actgctcact gccccggcgc acaccacgtc cacgcgtctg cactactact
     8161 ccggccgggc actcgtcgtc ctcagtggtg gtcaagataa gaacgcaatc aatgaatcaa
     8221 tgggtgcagg cagcaggcag caggctttgg cttgacacac cagcgagaag atgccctcct
     8281 ccctccaact cttcctatat atatacacat gaaaccgtca tccatgtatc catccatcca
     8341 cacacacaaa cagacaagca tacaacaccg ttgccattta gctgttcttg ctaggcagtt
     8401 gcgcgcttac attgagccgc agctagctgt ctactacggc acaactatct cctgcgcgcg
     8461 tgctcgatcg atctgaaggc cagacctgtg cctgtccaat tttgccttgt tctacccacc
     8521 acaccagtag tcgagaaggt tccatataag cgcaagcaga cagcagcaaa agagctaagg
     8581 agagtctgag acaaggttcg aacagaatgg gtcagtccct tatgttacgt cctgtagaaa
     8641 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     8701 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
     8761 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
     8821 tctggtatca gcgcgaagtc tttataccga aaggtaagtc ttactctctc ttttttggtc
     8881 tgtattttta attttttgaa gtatactatt tgtactgacg ctaataatct tttttcaggt
     8941 tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg
     9001 gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc
     9061 acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg
     9121 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag
     9181 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc
     9241 acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac
     9301 cacgcgtctg ttgactggca ggtacttcat gcttcaacgt gtaacttaag agatactgtg
     9361 tgaaatttta tatttccata catttgcttg acctttgctt tttgtcaatt tttttcccct
     9421 tacaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
     9481 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
     9541 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
     9601 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
     9661 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
     9721 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
     9781 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
     9841 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
     9901 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
     9961 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
    10021 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
    10081 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
    10141 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
    10201 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
    10261 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
    10321 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
    10381 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
    10441 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
    10501 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
    10561 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat
    10621 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    10681 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    10741 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    10801 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    10861 gcgcggtgtc atctatgtta ctag
//
