LOCUS       sequence-468508-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pCER6:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..72
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      160..870
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1165..1956
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2297..2885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2786..2805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3221..4369
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4515..4539
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5041..5386
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5329..5349
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5477..6271
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5531..5550)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6141..6160
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6284..6536
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      9884..10136
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 acatgtgacc gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat
       61 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      121 tgaaaaggtt tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg
      181 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
      241 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
      301 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
      361 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
      421 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag
      481 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
      541 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
      601 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
      661 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
      721 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc
      781 tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg
      841 ccgcgaacgg cctcacccca aaaatggcag cgctggccaa ttcccgaggc acgaacccag
      901 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
      961 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     1021 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     1081 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     1141 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     1201 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1261 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt
     1321 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1381 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1441 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1501 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1561 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1621 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1681 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1741 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1801 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1861 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac
     1921 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     1981 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     2041 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     2101 ctacacaaat tgggagatat atcatgctgt cagaccaagt ttactcatat atactttaga
     2161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa
     2941 cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca
     3001 ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat
     3061 tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga
     3121 taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat
     3181 tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg
     3241 gaaggcatac aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat
     3301 cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt
     3361 ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc
     3421 cgcgccatcg gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc
     3481 gaagtcgatg accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg
     3541 cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa
     3601 gcagcagatc aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat
     3661 gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat
     3721 cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac
     3781 ctacaccggc gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga
     3841 gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca
     3901 ggacctgggc tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg
     3961 cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc
     4021 gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct
     4081 gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga
     4141 gaagtaccgc aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg
     4201 ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg
     4261 cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct
     4321 ggtggaacac gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg
     4381 gtcagttccg gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc
     4441 atggttgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     4501 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     4561 aataacacat tgcggacgtt tttaatgtac tggggttgaa cactctgtgg gtctcatgcc
     4621 gaattcggat ccggaggtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     4681 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
     4741 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     4801 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     4861 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     4921 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgataaca tggtggagca
     4981 cgacactctg gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat
     5041 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat
     5101 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
     5161 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
     5221 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
     5281 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5341 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag
     5401 agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt
     5461 tacaattatc gatacaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5641 tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6001 ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa
     6061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6241 cttctatcgc cttcttgacg agttcttctg agcttgtcaa gcagatcgtt caaacatttg
     6301 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     6361 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6421 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6481 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
     6541 ctgcaagaat tcaagcttgg agtctatatt tgacatttca aagtttaaca acatagttct
     6601 aaataattac ctaaatttta agtcaaatgt gaattatatt ttactcttcg atatcggttg
     6661 ttgacgatta accatgcaaa aaagaaacat taattgcgaa tgtaaataac aaaacatgta
     6721 actcttgtag atatacatgt atcgacattt aaacccgaat atatatgtat acctataatt
     6781 tctctgattt tcacgctacc tgccacgtac atgggtgata ggtccaaact cacaagtaaa
     6841 agtttacgta cagtgaattc gtctttttgg gtataaacgt acatttaatt tacacgtaag
     6901 aaaggattac caattctttc atttatggta ccagacagag ttaaggcaaa caagagaaac
     6961 atatagagtt ttgatatgtt ttcttggata aatattaaat tgatgcaata tttagggatg
     7021 gacacaaggt aatatatgcc ttttaaggta tatgtgctat atgaatcgtt tcgcatgggt
     7081 actaaaatta tttgtcctta ctttatataa acaaattcca acaaaatcaa gtttttgcta
     7141 aaactagttt atttgcgggt tatttaatta cctatcatat tacttgtaat atcattcgta
     7201 tgttaacggg taaaccaaac caaaccggat attgaactat taaaaatctt gtaaatttga
     7261 cacaaactaa tgaatatcta aattatgtta ctgctatgat aacgaccatt tttgtttttg
     7321 agaaccataa tataaattac aggtacgtga caagtactaa gtatttatat ccacctttag
     7381 tcacagtacc aatattgcgc ctaccgggca acgtgaacgt gatcatcaaa tcaaagtagt
     7441 taccaaacgc tttgatctcg ataaaactaa aagctgacac gtcttgctgt ttcttaattt
     7501 atttctctta caacgacaat tttgagaaat atgaaatttt tatatcgaaa gggaacagtc
     7561 cttatcattt gctcccatca cttgcttttg tctagttaca actggaaatc gaagagaagt
     7621 attacaaaaa catttttctc gtcatttata aaaaaatgac aaaaaattaa atagagagca
     7681 aagcaagagc gttgggtgac gttgctctct tcattaactc ctctcatcta ccccttcctc
     7741 tgttcgcctt tatatccttc accttccctc tctcatcttc attaactcat cttcaaaaat
     7801 accctaatca cattttgtaa caataataca attatacatt aaaactctcc gacgaatggg
     7861 tcagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
     7921 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
     7981 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
     8041 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
     8101 aggtaagtct tactctctct tttttggtct gtatttttaa ttttttgaag tatactattt
     8161 gtactgacgc taataatctt ttttcaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
     8221 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
     8281 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
     8341 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
     8401 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     8461 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     8521 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtacttcatg
     8581 cttcaacgtg taacttaaga gatactgtgt gaaattttat atttccatac atttgcttga
     8641 cctttgcttt ttgtcaattt ttttcccctt acaggtggtg gccaatggtg atgtcagcgt
     8701 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt
     8761 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
     8821 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
     8881 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
     8941 tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga
     9001 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
     9061 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
     9121 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
     9181 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
     9241 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
     9301 ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa
     9361 actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga
     9421 taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca
     9481 aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga
     9541 gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca
     9601 ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca
     9661 ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt
     9721 tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga
     9781 ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg
     9841 tgaaaaaccg cagcagggag gcaaacaatg agcttgtcaa gcagatcgtt caaacatttg
     9901 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     9961 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
    10021 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
    10081 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
    10141 ctactagagg atgc
//
