LOCUS       sequence-46856-e        1911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Nagaya H, Tamura T, Higa-Nishiyama A, Ohashi K, Takeuchi M, 
            Hashimoto H, Hatsuzawa K, Kinjo M, Okada T, Wada I
  TITLE     Regulated motion of glycoproteins revealed by direct visualization 
            of a single cargo in the endoplasmic reticulum.
  JOURNAL   J Cell Biol. 2008 Jan 14;180(1):129-43.
  PUBMED    18195104
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2008-01-14"; volume: "180(1)"; pages: "129-43"
COMMENT     Sequence Label: Ch-gelsolN
FEATURES             Location/Qualifiers
     source          1..1911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1195..1204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1201..1911
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDQMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1346..1364)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1614..1633
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcatgg tggtggaaca ccccgagttc ctcaaggcag ggaaggagcc tggcctgcag
       61 atctggcgtg tggagaagtt cgatctggtg cccgtgccca ccaaccttta tggagacttc
      121 ttcacgggcg acgcctacgt catcctgaag acagtgcagc tgaggaacgg aaatctgcag
      181 tatgacctcc actactggct gggcaatgag tgcagccagg atgagagcgg ggcggccgcc
      241 atctttaccg tgcagctgga tgactacctg aacggccggg ccgtgcagca ccgtgaggtc
      301 cagggcttcg agtcggccac cttcctaggc tacttcaagt ctggcctgaa gtacaagaaa
      361 ggaggtgtgg catcaggatt caagcacgtg gtacccaacg aggtggtggt gcagagactc
      421 ttccaggtca aagggcggcg tgtggtccgt gccaccgagg tacctgtgtc ctgggagagc
      481 ttcaacaatg gcgactgctt catcctggac ctgggcaaca acatccacca gtggtgtggt
      541 tccaacagca atcggtatga aagactgaag gccacacagg tgtccaaggg catccgggac
      601 aacgagcgga gtggccgggc ccgagtgcac gtgtctgagg agggcactga gcccgaggcg
      661 atgctccagg tgctgggccc caagccggct ctgcctgcag gtaccgagga caccgccaag
      721 gaggatgcgg ccaaccgcaa gctggccaag ctctacaagg tctccaatgg tgcagggacc
      781 atgtccgtct ccctcgtggc tgatgagaac cccttcgccc agggggccct gaagtcagag
      841 gactgcttca tcctggacca cggcaaagat gggaaaatct ttgtctggaa aggcaagcag
      901 gcaaacacgg aggagaggaa ggctgccctc aaaacagcct ctgacttcat caccaagatg
      961 gactacccca agcagactca ggtctcggtc cttcctgagg gcggtgagac cccactgttc
     1021 aagcagttct tcaagaactg gcgggaccca gaccagacag atggcctggg cttgtcctac
     1081 ctttccagcc atatcgccaa cgtggagcgg gtgcccttcg acgccgccac cctgcacacc
     1141 tccaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     1201 atggtgagca agggcgagga ggaccagatg gccatcatca aggagttcat gcgcttcaag
     1261 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1321 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     1381 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1441 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1501 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     1561 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1621 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1681 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     1741 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1801 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1861 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta a
//
