LOCUS       sequence-468604-       13682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13682)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-497    C-Tag GFP[2] L32 Piezo1 EBPF2
FEATURES             Location/Qualifiers
     source          1..13682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8841..9415,9416..9418,9419..9427)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9008..9025)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9235..9254
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9428..9430,9431..9433,9434..10147)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9473..9494)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          10193..10325
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10334..10922
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10387..10407)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10805..10816)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10949..10966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10955..11066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11162..11182)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11162..11180)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11201..11217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11201..11217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11214..11236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11319..11338)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11384..11439
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11438..11457)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11818..12406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11898..11917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12577..13368,13369..13437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13200..13219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13438..13542)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13610..13628
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggag ccgcacgtgc tgggcgccgg gctctactgg ctgttgctgc cctgcacgct
     1081 cctggcggcc tccctgttac gcttcaatgc tctctcgctg gtctatttgc tgtttctact
     1141 gctgctgccc tggcttccag gcccctcaag acacagcata ccaggtcaca caggtcgcct
     1201 gctccgtgca ctactctgcc tcagcctcct cttcctggtg gcccaccttg cctttcagat
     1261 atgcctacac accgtgcctc acctggacca gtttctggga caaaacggta gcctttgggt
     1321 gaaggtgtct caacacatag gggttacgag gctggacctg aaggacatct ttaacaccac
     1381 caggctggta gcacctgacc tgggagtgct gctggcgtcc tccctttgcc ttggcctctg
     1441 tggacgcctc acgaggaaag ccgggcagag tcggcgcacc caggagctgc aggatgatga
     1501 cgatgacgat gatgacgacg atgaagacat agatgctgcc ccagccgtgg ggctgaaggg
     1561 agcccctgcc ctggcaacca aacgcaggct gtggctggcc tcccgcttcc gggtcacggc
     1621 ccactggctg ctgatgacct ctggacggac gctggtcatt gtgttgctgg ccctggcagg
     1681 catagcccac ccttcggcct tctccagcat ctacctggtg gtgttcctgg ccatctgcac
     1741 ctggtggtcc tgtcactttc ctctcagccc cctgggcttt aacaccctct gtgtcatggt
     1801 gagctgtttt ggtgccggcc atctcatttg cctatactgc tatcagacac catttatcca
     1861 ggacatgtta ccccctggga acatctgggc caggctattt ggtctcaaga acttcgtaga
     1921 cctccctaac tactccagcc ccaacgccct ggtactcaac actaagcacg cctggcccat
     1981 ctatgtgagt cccggaatcc tgctgctgct atattacaca gccacctctc tcctgaagct
     2041 ccacaagagc tgtccctcag agctgaggaa ggagacaccc agggaggatg aggagcatga
     2101 gctggaactg gaccacctgg agccagagcc ccaggctagg gacgccaccc agggtgagat
     2161 gcccatgacc acggaacctg accttgacaa ctgcaccgtg catgtactaa ccagccagag
     2221 ccctgtccgc cagcgtccag ttcgccccag gctggctgag ctgaaagaga tgtcaccgct
     2281 acatggcctg ggccacctca tcatggacca gagctatgtg tgtgccctga ttgccatgat
     2341 ggtgtggagc atcatgtacc acagttggct gaccttcgtc ctgctgctct gggcctgcct
     2401 catctggacg gtgcgtagcc gtcaccagct ggctatgctc tgctcgccct gcatcctgct
     2461 gtatgggctg acgctctgct gcctgaggta tgtgtgggcc atggaacttc ctgagctgcc
     2521 caccaccctg ggccctgtca gcctgcacca gttgggactg gaacacacac gctacccttg
     2581 cctggacctc ggtgccatgc tcctctatct gctcacattc tggctccttc tgcgtcagtt
     2641 cgtgaaggag aagctgctga agaagcaaaa ggtgcctgcg gcgctgctgg aggtcacggt
     2701 ggccgacact gagcccacac agacccagac gctgctgcgg agcctggggg agctggtcac
     2761 cggcatctac gtcaaatact ggatctatgt gtgcgccggc atgttcattg tggtcagctt
     2821 cgccggccgc ctggtggtct acaaaatcgt ctacatgttc ctcttcctgc tgtgcctcac
     2881 cttgttccag gtctactaca ccctgtggag gaagctgcta cgtgtcttct ggtggctcgt
     2941 ggtggcctat acaatgctcg tgctcatcgc tgtgtacacc ttccagttcc aggacttccc
     3001 cacctattgg cgcaacctca cgggcttcac agacgagcag ttgggcgacc tgggcctgga
     3061 gcagttcagt gtgtcggagc tcttttccag tatcctcatc cctggcttct tcctgctggc
     3121 ctgcatcctg cagctgcact acttccacag accgttcatg cagctcactg acctggagca
     3181 cgtgccgcca ccaggcaccc gccaccctcg atgggctcac aggcaggatg cagtgagcga
     3241 ggcccctctg cttgagcatc aggaggaaga ggaagtcttc agggaagatg ggcagagcat
     3301 ggatgggccc caccaggcca cacaggtccc tgagggtacg gccagcaagt ggggcctggt
     3361 ggctgaccgg ctgctggacc tagcggccag cttctcggct gtcctcaccc gaatccaggt
     3421 gttcgttcgg cgcttgctag aacttcacgt cttcaagctg gtggccctct acactgtctg
     3481 ggtggccctg aaagaagtgt ctgtgatgaa cctgctgctg gtggtgctat gggccttcgc
     3541 cttgccctat ccgcgcttcc ggcccatggc ttcctgcctg tccaccgtgt ggacctgtat
     3601 catcattgta tgcaagatgc tctatcagct caagattgtc aacccgcatg agtactccag
     3661 caactgcact gagcccttcc ccaacaatac caacttgcag cctttggaga tcaaccagtc
     3721 tttgctgtac cgtggccctg ttgaccctgc caactggttt ggggtgcgga agggttaccc
     3781 caacttgggc tatatccaga accacctgca gatccttctg ttgctggtgt ttgaggccgt
     3841 ggtgtaccgg cgccaagagc actaccgccg gcagcaccag caggcccctc tgcccgccca
     3901 ggctgtgtgc gcagatggca cccgccagag gttggaccag gacctactta gctgcctcaa
     3961 gtatttcatc aacttcttct tctacaaatt cgggctggag atctgcttct tgatggccgt
     4021 gaatgtgatt gggcagcgta tgaacttcat ggtgatctta cacggttgct ggttggtggc
     4081 catccttaca cgccggcgcc gtgaggccat cgcccgcctc tggcctaact actgtctgtt
     4141 cctcacgctg ttcctgctgt accagtacct gctgtgtttg ggcatgcccc ctgcactgtg
     4201 cattgactat ccatggcgct ggagcaaggc catccccatg aattccgccc tcatcaagtg
     4261 gctgtaccta cctgacttct tcagagcccc caactccacc aaccttatca gtgacttcct
     4321 cctgctgctt tgcgcctccc agcagtggca ggtcttctca gcggagcgaa cggaggagtg
     4381 gcaacgcatg gcgggcatca acactgacca cctggagccc ctgcgtgggg agcccaaccc
     4441 tatacccaac ttcatccact gcaggtccta tctggatatg ctgaaggtgg ccgtcttccg
     4501 ctacctgttc tggctggtgc tcgttgtggt gtttgttgcg ggggccaccc gcataagcat
     4561 cttcgggctg gggtacctgc tagcctgctt ctacctgctg ctgtttggca ctaccctgct
     4621 gcagaaggac acgcgagccc agctcgtgct gtgggactgc ctcatcctct ataatgtcac
     4681 tgtcatcatc tctaagaata tgctgtcgct cctgtcctgt gtcttcgtgg agcaaatgca
     4741 gagcaacttc tgctgggtca tccagctctt cagcctcgtg tgcacagtca aaggctacta
     4801 tgatcccaaa gagatgatga ccagggaccg ggactgcctg ctgcctgtgg aggaggccgg
     4861 gatcatctgg gacagtatct gcttcttctt cctgctcttg caacggcgca tctttctcag
     4921 ccactacttc ctgcatgtca gcgctgacct gaaagccaca gccctgcagg catccagggg
     4981 ctttgccctc tacaatgcag ccaacctgaa gagcatcaac ttccatcgcc agattgagga
     5041 gaagtccctg gcccagctga aaagacagat gaagcgcatc cgtgccaaac aggagaagta
     5101 caggcagagc caggcaagtc gtggccaact ccagtccaaa gaccctcagg atcccagcca
     5161 ggagccaggg cctgacagcc cagggggctc ctccccgcca cggagacagt ggtggcgccc
     5221 ctggctggac cacgccacag tcatccactc tggcgactac ttcctgtttg agtcagatag
     5281 cgaggaggaa gaggaggccc tacctgagga ccccaggcct gcagctcaga gtgccttcca
     5341 gatggcatac caggcatggg taaccaatgc ccagacagtg ctgaggcagc gtcgggagcg
     5401 ggcacggcag gagcgggcag agcagctggc ttctggaggt gacttgaacc cagatgtgga
     5461 accagtagat gtcccagaag atgagatggc aggccgtagc cacatgatgc agcgtgtgct
     5521 aagcaccatg cagttcctgt gggtgctggg ccaggccacg gtagacgggc tgacgcgctg
     5581 gctgcgtgca ttcacgaagc accaccgcac catgagcgat gtgctgtgcg cagagcgcta
     5641 cctgctcacc caggagcttc ttcgggttgg agaggtacgc cgaggtgtgc tggaccagct
     5701 ttatgtgggt gaagatgagg ccacattgtc aggtcccgtg gagacccggg atggacccag
     5761 cacagcctca agtgggctgg gagccgaaga gcctttgagt agcatgacag acgacaccag
     5821 cagccccctg agcacaggct ataacacccg cagtggcagt gaggagattg tcaccgacgc
     5881 tggggacctc caggctggga cctccctgca cggctcccaa gagcttttag ccaatgctcg
     5941 tacccggatg cgcacggcca gcgagctgct actggatagg cgcctgcata tccctgagct
     6001 ggaggaggcc gagcggtttg aggcacagca gggccggact ctgcggctgc tcagggctgg
     6061 gtaccagtgc gtggcggcac actcggagct gctctgttac ttcatcatca tccttaacca
     6121 catggtgaca gcctcggctg cctccctggt gctgcccgtg cttgtgttcc tgtgggccat
     6181 gctgaccatc ccgaggccta gcaagcgctt ttggatgaca gctatcgtct tcactgaggt
     6241 catggtggtc accaaatacc tgttccagtt cggcttcttc ccctggaaca gctacgttgt
     6301 gctgcggcgc tatgagaaca agccctactt ccctccgcga atcctgggcc ttgagaaaac
     6361 ggacagctac atcaagtatg acctggtgca gctcatggcc ctcttcttcc accgctcgca
     6421 gctactgtgt tatggcctct gggaccatga ggaggatcgc tatcccaagg accattgcag
     6481 gagtagtgtg aaggaccggg aggccaagga agagccagaa gctaagctgg aatcgcagtc
     6541 tgagacaggc actgggcatc ccaaggagcc agtgttggcc ggtactccca gggaccacat
     6601 ccaagggaaa ggaagtatta gatccaagga tgttatccaa gatcccccag aggaccttaa
     6661 gccccggcac acgaggcaca tcagcatacg cttcaggagg cgcaaggaga ctccaggacc
     6721 caaaggaaca gcagtcatgg agactgagca cgaggaggga gaaggaaaag aaactacaga
     6781 gagaaagagg ccgcgtcaca ctcaagaaaa atcgaagttt cgggagagaa tgaaggcagc
     6841 tgggcgccgg ctgcagagct tctgtgtgtc actggcccag agcttctacc aacccttgca
     6901 gcgtttcttc catgacattc tgcacacaaa gtaccgggcg gccaccgacg tctacgccct
     6961 catgttcctg gccgatattg tcgacatcat catcatcatc tttggttttt gggcttttgg
     7021 gaagcactct gcagccacag acattgcatc ctcgctgtca gatgaccagg tgccacaggc
     7081 cttcctgttc atgctgctgg tccagtttgg caccatggtc atcgaccgtg ccctctacct
     7141 gcgcaagact gtcctgggaa agctggcctt tcaggtggtc ctggtggtgg cgattcacat
     7201 ctggatgttc tttatcttac cggctgtcac tgagaggatg ttcagccaga atgcggtggc
     7261 acagctgtgg tacttcgtca agtgcattta ctttgccctg tccgcctacc agatccgctg
     7321 tggctacccc acccgtatct tgggcaactt cctcaccaag aaatacaacc atctaaacct
     7381 cttccttttc caggggttcc gtctagtgcc gttcctggtg gagctgcggg ccgtcatgga
     7441 ctgggtgtgg accgacacca cgctgtccct gtccaactgg atgtgtgtgg aagacatcta
     7501 tgccaacatc tttatcatca agtgcagccg agagacagag aagaaatacc cccagcccaa
     7561 ggggcagaag aagaagaaaa ttgtcaagta tggtatggga ggcctcatta tcctcttcct
     7621 catcgccatc atctggttcc ctctgctctt catgtcactg atccgctctg tggtcggggt
     7681 cgtcaaccag cccattgatg tcaccgtcac cctcaagcta ggcggctatg agccactgtt
     7741 caccatgagc gcccagcagc catccattgt gccattcaca ccccaggcct acgaggagct
     7801 gtcccagcag tttgacccct atccactagc catgcagttc attagccagt acagtcctga
     7861 ggacatcgtc actgcacaga tcgagggcag ctcgggggcg ctgtggcgca tcagcccacc
     7921 cagccgagcc cagatgaagc aggagctgta caacggcaca gccgacatta cactgcgctt
     7981 tacctggaat ttccaaaggg acctggccaa gggtggcact gtggagtata ctaatgagaa
     8041 gcacaccttg gagctggccc ccaacagtac ggcacgaagg cagctggccc aactgctcga
     8101 gggcagacct gaccagtcag tggtcattcc ccatctcttc cccaagtaca ttcgtgctcc
     8161 caatgggcct gaagccaacc ctgtgaagca gctgcagcca gatgaggaag aggactacct
     8221 tggtgtgcgc atccagctgc ggagggagca agtgggcaca ggggcctctg gggagcaagc
     8281 gggcaccaag gcctccgact tcctcgagtg gtgggtcatc gagctgcagg actgcaaggc
     8341 tgactgcaac ctgctgccca tggtcatctt cagtgacaag gtcagcccac ctagcctggg
     8401 cttcctggcc ggctacggga ttgtggggct gtatgtctcc atcgtgctgg tggttggcaa
     8461 gtttgtgcgg ggcttcttca gcgagatctc tcactccatc atgttcgagg aactgccgtg
     8521 tgtggaccgc atcctcaagc tgtgccagga catcttcttg gtgcgcgaga cccgggagct
     8581 ggagctggag gaggagctat acgccaagct catcttcctg taccgatctc cagagaccat
     8641 gattaagtgg acacgtgaga gggaggacgt gggtggcgga gggagcgaag gcggcggatc
     8701 cggagggcct ggctctggcg gagaggggag cgctggaggc gggtcagccg gtggtgggag
     8761 tgaaaagagg gaccacatgg tgctgctcga gtacgtcaca gcagctggca tcaccgatgc
     8821 cagctaagaa ttcagatccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     8881 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8941 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     9001 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     9061 ctggtagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     9121 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     9181 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     9241 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     9301 tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     9361 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     9421 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     9481 gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg agggcgatgc
     9541 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     9601 gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct accccgacca
     9661 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     9721 catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     9781 caccctggtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg gcaacatcct
     9841 ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg ccgtcaagca
     9901 gaagaacggc atcaaggtga acttcaagat ccgccacaac gtggaggacg gcagcgtgca
     9961 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
    10021 cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca
    10081 catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg acgagctgta
    10141 caagtaaacc ggtgctcgag gtaccgatga atagctaagg tcgaggccgc aggtaagtat
    10201 caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag
    10261 actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc
    10321 cacaggtgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    10381 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    10441 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    10501 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    10561 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    10621 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    10681 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
    10741 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    10801 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10861 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10921 gcctggaatt cgagctcggt acgatcagcc tcgactgtgc cttctagttg ccagccatct
    10981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    11041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    11101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagccc agcttttgtt
    11161 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    11221 gaaattgtta tccgctaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
    11281 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
    11341 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
    11401 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
    11461 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
    11521 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
    11581 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
    11641 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
    11701 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
    11761 agatcggccg gccgccggcg tcgacgcgcg cggccgcggc gcgccgttgc tggcgttttt
    11821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    12001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    12061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    12121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    12181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12241 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12661 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12721 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12781 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12841 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12901 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12961 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    13021 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    13081 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    13141 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    13201 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    13261 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    13321 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    13381 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    13441 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    13501 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13561 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    13621 cacgaggccc tttcgtctac gcgcgcgccg gcggcgcgcc gtcgacggcc ggccgcggcc
    13681 gc
//
