LOCUS       sequence-468641-        9923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA019
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    385..519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(662..684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1244..3033
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3335..5260)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        5781..5885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5886..5954,5955..6746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6104..6123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6917..7505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7406..7425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7659..7676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7757..7776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7795..7815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7800..8129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7980..8115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8222..8821
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8222..8241)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8718..8738
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9051..9185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9250..9269)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9294..9342
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag cggccgcgtt taaacttaat taaggcgcgc catgcgcacg
      361 tataccacgt gcttaagttc gaaggatcca gacatgataa gatacattga tgagtttgga
      421 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      481 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      541 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      601 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
      661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      781 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
      841 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
      901 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
      961 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     1021 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     1081 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     1141 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     1201 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     1261 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     1321 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     1381 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     1441 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     1501 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     1561 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     1621 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     1681 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     1741 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     1801 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     1861 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     1921 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     1981 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     2041 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     2101 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     2161 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     2221 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     2281 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     2341 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     2401 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     2461 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     2521 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     2581 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     2641 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     2701 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     2761 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     2821 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     2881 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
     2941 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
     3001 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
     3061 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
     3121 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
     3181 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
     3241 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
     3301 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
     3361 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
     3421 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
     3481 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
     3541 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
     3601 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
     3661 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
     3721 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
     3781 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
     3841 aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac
     3901 cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta
     3961 tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat
     4021 aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg
     4081 gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac
     4141 gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc
     4201 ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac
     4261 tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct gcccctcctc
     4321 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     4381 ctcctgcccc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     4441 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     4501 ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     4561 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgctc
     4621 ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
     4681 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc
     4741 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     4801 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     4861 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctgccc
     4921 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc
     4981 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
     5041 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
     5101 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
     5161 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
     5221 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
     5281 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
     5341 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
     5401 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
     5461 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
     5521 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
     5581 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
     5641 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
     5701 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6121 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6421 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7681 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7741 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ctgtggaatg
     7801 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7861 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7921 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8041 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8101 gcttttttgg aggcctaggc ttttgcaaaa agcttgcatg cctgcaggtc ggccgccacg
     8161 accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga gacgaccttc
     8221 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     8281 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     8341 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8401 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8461 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8521 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8581 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8641 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8701 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8761 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8821 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
     8881 ccgaagccac ccggggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
     8941 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9001 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9061 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9121 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9181 ggatcactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg ggagatgggg
     9241 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     9301 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     9361 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     9421 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     9481 acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga cggggtcccc
     9541 catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt
     9601 ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca tggaccgcat
     9661 gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg acccccaaaa
     9721 accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt ttgacagctt
     9781 atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg
     9841 gaagatctct agaagctggg taccagctgc tagcaagctt gctagcggcc gaggcgtatc
     9901 acgaggccct ttcgtcttca ctc
//
