LOCUS       sequence-468710-        1994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO276
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..33
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(350..384)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(455..1114)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1029..1048
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1296..1884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1785..1804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtctcata tgcccaagaa gaagcgtaaa gtgccggcgg atgctctaga cgatttcgat
       61 ctggatatgc taccagccga tgcacttgac gatttcgact tagacatgct tccggccgac
      121 gctctagatg acttcgactt agatatgctc ggttccggcg cgaacaatac accatttccg
      181 ggatattttg ggggccttga tttattcgac tacgacttcc ttttcggcaa cgactttgct
      241 ggctcaggtg cgaataatac tccttttcca ggttattttg gaggtttgga tttatttgat
      301 tatgactttt tgtttggcaa tgactttgct ggttcttgag accagaccaa taaaaaacgc
      361 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      421 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      481 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      541 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
      601 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
      661 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
      721 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
      781 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
      841 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
      901 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
      961 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1021 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1081 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1141 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1201 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     1261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1981 cgtagcatcg tctc
//
