LOCUS       sequence-468713-        1909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO275
FEATURES             Location/Qualifiers
     source          1..1909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..32
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(259..293)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(364..1023)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     938..957
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1205..1793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1694..1713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtctcatat gcccaagaag aagcgtaaag tgccggcgga tgctctagac gatttcgatc
       61 tggatatgct accagccgat gcacttgacg atttcgactt agacatgctt ccggccgacg
      121 ctctagatga cttcgactta gatatgctcg gctcaggtgc gaataatact ccttttccag
      181 gttattttgg aggtttggat ttatttgatt atgacttttt gtttggcaat gactttgctg
      241 gttcttgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      301 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      361 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      421 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      481 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      541 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
      601 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      661 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      721 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      781 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      841 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      901 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      961 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1021 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1081 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
     1141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1321 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1861 ctgcgttatc ccctgattct gtggataacc gtagtcgggc atcgtctca
//
