LOCUS       sequence-469243-        2988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for EED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pCDS.EED
FEATURES             Location/Qualifiers
     source          1..2988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1353..1387)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1458..2117)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2032..2051
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2299..2887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2788..2807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgtccgaga gggaagtgtc gactgcgccg gcgggaacag acatgcctgc
       61 ggccaagaag cagaagctga gcagtgacga gaacagcaat ccagacctct ctggagatga
      121 gaatgatgac gctgtcagta tagaaagtgg tacaaacact gaacgccctg atacacctac
      181 aaacacgcca aatgcacctg gaaggaaaag ttggggaaag ggaaaatgga agtcaaagaa
      241 atgcaaatat tctttcaaat gtgtaaatag tctcaaggaa gatcataacc aaccattgtt
      301 tggagttcag tttaactggc acagtaaaga aggagatcca ttagtgtttg caactgtagg
      361 aagcaacaga gttaccttgt atgaatgtca ttcacaagga gaaatccggt tgttgcaatc
      421 ttacgtggat gctgatgctg atgaaaactt ttacacttgt gcatggacct atgatagcaa
      481 tacgagccat cctctgctgg ctgtagctgg atctagaggc ataattagga taataaatcc
      541 tataacaatg cagtgtataa agcactatgt tggccatgga aatgctatca atgagctgaa
      601 attccatcca agagatccaa atcttctcct gtcagtaagt aaagatcatg ctttacgatt
      661 atggaatatc cagacggaca ctctggtggc aatatttgga ggcgtagaag ggcacagaga
      721 tgaagttcta agtgctgatt atgatctttt gggtgaaaaa ataatgtcct gtggtatgga
      781 tcattctctt aaactttgga ggatcaattc aaagagaatg atgaatgcaa ttaaggaatc
      841 ttatgattat aatccaaata aaactaacag gccatttatt tctcagaaaa tccattttcc
      901 tgatttttct accagagaca tacataggaa ttatgttgat tgtgtgcgat ggttaggcga
      961 tttgatactt tctaagtctt gtgaaaatgc cattgtgtgc tggaaacctg gcaagatgga
     1021 agatgatata gataaaatta aacccagtga atctaatgtg actattcttg ggcgatttga
     1081 ttacagccag tgtgacattt ggtacatgag gttttctatg gatttctggc aaaagatgct
     1141 tgcattgggc aatcaagttg gcaaacttta tgtttgggat ttagaagtag aagatcctca
     1201 taaagccaaa tgtacaacac tgactcatca taaatgtggt gctgctattc gacaaaccag
     1261 ttttagcagg gatagcagca ttcttatagc tgtttgtgat gatgccagta tttggcgctg
     1321 ggatcgactt cgaggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg
     1381 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     1441 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     1501 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     1561 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     1621 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     1681 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     1741 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     1801 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     1861 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1921 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     1981 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     2041 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     2101 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     2161 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     2221 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2281 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2341 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2401 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2461 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2521 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2581 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2641 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2701 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2761 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2821 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2881 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2941 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
