LOCUS       sequence-469245-        3882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for SUZ12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pCDS.SUZ12
FEATURES             Location/Qualifiers
     source          1..3882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2247..2281)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2352..3011)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2926..2945
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3193..3781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3682..3701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggcgcctc agaagcacgg cggtggggga gggggcggct cggggcccag
       61 cgcggggtcc gggggaggcg gcttcggggg ttcggcggcg gtggcggcgg cgacggcttc
      121 gggcggcaaa tccggcggcg ggagctgtgg agggggtggc agttactcgg cctcctcctc
      181 ctcctccgcg gcggcagcgg cgggggctgc ggtgttaccg gtgaagaagc cgaaaatgga
      241 gcacgtccag gctgaccacg agcttttcct ccaggccttt gagaagccaa cacaaatcta
      301 tagatttctt cgaactcgga atctcatagc accaatattt ttgcacagaa ctcttactta
      361 catgtctcat cgaaactcca gaacaaacat caaaaggaaa acatttaaag ttgatgatat
      421 gttatcaaaa gtagagaaaa tgaaaggaga gcaagaatct catagcttgt cagctcattt
      481 gcagcttacg tttactggtt tcttccacaa aaatgataag ccatcaccaa actcagaaaa
      541 tgaacaaaat tctgttaccc tggaagtcct gcttgtgaaa gtttgccaca aaaaaagaaa
      601 ggatgtaagt tgtccaataa ggcaagttcc cacaggtaaa aagcaggtgc ctttgaatcc
      661 tgacctcaat caaacaaaac ccggaaattt cccgtccctt gcagtttcca gtaatgaatt
      721 tgaacctagt aacagccata tggtgaagtc ttactcgttg ctatttagag tgactcgtcc
      781 aggaagaaga gagtttaatg gaatgattaa tggagaaacc aatgaaaata ttgatgtcaa
      841 tgaagagctt ccagccagaa gaaaacgaaa tcgtgaggat ggggaaaaga catttgttgc
      901 acaaatgaca gtatttgata aaaacaggcg cttacagctt ttagatgggg aatatgaagt
      961 agccatgcag gaaatggaag aatgtccaat aagcaagaaa agagcaacat gggagactat
     1021 tcttgatggg aagaggctgc ctccattcga aacattttct cagggaccta cgttgcagtt
     1081 cactcttcgt tggacaggag aaaccaatga taaatctacg gctcctattg ccaaacctct
     1141 tgccactaga aattcagaga gtctccatca ggaaaacaag cctggttcag ttaaacctac
     1201 tcaaactatt gctgttaaag aatcattgac tacagaccta caaacaagaa aagaaaagga
     1261 tactccaaat gaaaaccgac aaaaattaag aatattttat cagtttctct ataacaacaa
     1321 tacaaggcaa caaactgaag caagagatga cctgcattgc ccttggtgta ctctgaactg
     1381 ccgcaaactt tatagtttac tcaagcatct taaactctgc catagcagat ttatcttcaa
     1441 ctatgtttat catccaaaag gtgctaggat agatgtttct atcaatgagt gttatgatgg
     1501 ctcctatgca ggaaatcctc aggatattca tcgccaacct ggatttgctt ttagtcgcaa
     1561 cggaccagtt aagagaacac ctatcacaca tattcttgtg tgcaggccaa aacgaacaaa
     1621 agcaagcatg tctgaatttc ttgaatctga agatggggaa gtagaacagc aaagaacata
     1681 tagtagtggc cacaatcgtc tgtatttcca tagtgatacc tgcttacctc tccgtccaca
     1741 agaaatggaa gtagatagtg aagatgaaaa agatcctgaa tggctaagag aaaaaaccat
     1801 tacacaaatt gaagagtttt ctgatgttaa tgaaggagag aaagaagtga tgaaactctg
     1861 gaatctccat gtcatgaagc atgggtttat tgctgacaat caaatgaatc atgcctgtat
     1921 gctgtttgta gaaaattatg gacagaaaat aattaagaag aatttatgtc gaaacttcat
     1981 gcttcatcta gtcagcatgc atgactttaa tcttattagc ataatgtcaa tagataaagc
     2041 tgttaccaag ctccgtgaaa tgcagcaaaa attagaaaag ggggaatctg cttcccctgc
     2101 aaacgaagaa ataactgaag aacaaaatgg gacagcaaat ggatttagtg aaattaactc
     2161 aaaagagaaa gctttggaaa cagatagtgt ctcaggggtt tcaaaacaga gcaaaaaaca
     2221 aaaactcgga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     2281 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     2341 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     2401 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     2461 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     2521 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     2581 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     2641 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     2701 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     2761 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     2821 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     2881 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     2941 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     3001 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     3061 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     3121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3841 gttctttcct gcgttatccc ctgattctgt ggataaccgt ag
//
