LOCUS       sequence-469315-        5373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for RSV B1 G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, 
            Boeckh MJ, King NP, Greninger AL, Bloom JD
  TITLE     RSV F evolution escapes some monoclonal antibodies but does not 
            strongly erode neutralization by human polyclonal sera.
  JOURNAL   J Virol. 2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25.
  PUBMED    40607811
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25."
COMMENT     Sequence Label: HDM_RSV_B1_G_31AACTdel
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1253..1632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1633..1836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1787..1807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1833..1857
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1950..2425
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(3314..3330)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3315..3331)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3424..3818
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(4152..4170)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4238..4342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4343..4411,4412..5203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4561..4580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cggagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
      661 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
      721 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
      781 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
      841 cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat
      901 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
      961 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     1021 aatcataatg gggaaggcca tccagcctcg cgtcggggag ctttttgcaa aagcctaggc
     1081 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     1141 ctgcataaat aaaaaaaatt agtcagccat gagcttggcc cattgcatac gttgtatcca
     1201 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
     1261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     1741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1801 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1861 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1921 ccctcgaagc tgatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
     1981 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
     2041 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
     2101 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
     2161 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
     2221 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
     2281 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
     2341 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
     2401 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
     2461 tcactttggc aaagaattcc gcgggcggcc gcgccaccat gctgaacctg aagtctatcg
     2521 cccagatcgc cctgagcgtg ctggccatga tcatcagcac cagcctgatc atcgccgcca
     2581 tcatctttat cattagcgct aatcacaagg tgaccctgac aaccgtgaca gtccagacca
     2641 tcaagaacca tacagagaag aacatcacca cctacctgac ccaagtgcct ccagaacggg
     2701 tgtccagcag caagcagcct acaaccacat ctcctatcca caccaattct gctacaacaa
     2761 gccctaatac caagtccgag acacaccaca caacagccca gaccaagggc agaaccacca
     2821 catctacaca aacaaacaag cctagcacca agcccagact caaaaacccc cccaagaaac
     2881 caaaggacga ttaccacttc gaggtgttca acttcgtgcc ctgcagcatc tgcggcaaca
     2941 accagctgtg caagagcatc tgtaaaacca tccccagcaa caagcctaag aagaagccta
     3001 caattaagcc caccaacaaa cctaccacca aaaccaccaa caagcgggac cccaagacac
     3061 ctgccaagac cacaaagaag gaaaccacaa caaatcctac caagaaacca accctgacca
     3121 ccaccgagag agataccagc acctctcaga gcaccgtgct ggacaccacc acactggaac
     3181 acaccatcca gcagcagtct ctgcacagca ccacccctga gaacacccct aacagcacac
     3241 agactcctac cgccagcgag cctagcacct ccaacagcac ccagaacaca cagtcccacg
     3301 cctgaaagct tatcgatacc gtcgacctcg agggcccaga tctaattcac cccaccagtg
     3361 caggctgcct atcagaaagt ggtggctggt gtggctaatg ccctggccca caagtatcac
     3421 taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac
     3481 tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac
     3541 atttattttc attgcaatga tgtatttaaa ttatttctga atattttact aaaaagggaa
     3601 tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga gctagttcaa accttgggaa
     3661 aatacactat atcttaaact ccatgaaaga aggtgaggct gcaaacagct aatgcacatt
     3721 ggcaacagcc cctgatgcct atgccttatt catccctcag aaaaggattc aagtagaggc
     3781 ttgatttgga ggttaaagtt ttgctatgct gtattttaca ttacttattg ttttagctgt
     3841 cctcatgaat gtcttttcac tacccatttg cttatcctgc atctctcagc cttgactcca
     3901 ctcagttctc ttgcttagag ataccacctt tcccctgaag tgttccttcc atgttttacg
     3961 gcgagatggt ttctcctcgc ctggccactc agccttagtt gtctctgttg tcttatagag
     4021 gtctacttga agaaggaaaa acagggggca tggtttgact gtcctgtgag cccttcttcc
     4081 ctgcctcccc cactcacagt gacccggaat ccctcgacat ggcagtctag atcattcttg
     4141 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4201 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4561 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4861 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5341 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
