LOCUS       sequence-469340-        2301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; ABA systems; Phlf-NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO174
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             620..646
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      complement(666..700)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(771..1430)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1345..1364
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1612..2200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2101..2120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggcacgta cgccctcacg ttccagcata ggtagcctta gatcaccaca
       61 tacgcacaaa gccatcctga cttcaactat tgaaatacta aaagaatgcg ggtattctgg
      121 actaagtatc gaatcagtgg ctagacgtgc cggggccagc aaacccacaa tttaccgttg
      181 gtggaccaac aaagccgctc taatagccga agtgtacgaa aacgagtctg aacaggtccg
      241 taagttccct gatttaggct cctttaaggc ggatttggat ttcttactga gaaacctatg
      301 gaaagtatgg agggagacaa tttgcggaga agcatttagg tgcgtcattg ctgaagccca
      361 acttgatccc gcgacgctta cacaactaaa agatcagttt atggagagaa ggcgtgaaat
      421 gccaaagaaa ttagtagaaa acgccatatc aaacggggag ctacccaaag acaccaacag
      481 agaactgcta ttagatatga tctttggttt ttgctggtac agactgttga ctgaacaact
      541 tactgtggag caagatatag aggagttcac ctttctgttg ataaacggag tttgcccagg
      601 gacacaacgt ggaagcggac tgcctccgtt agaaagacta acgctgggtt cttgagacca
      661 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      721 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      841 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      901 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      961 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1021 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1081 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1141 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1201 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1261 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1321 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1381 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1441 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1501 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2221 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2281 tgattctgtg gataaccgta g
//
