LOCUS       sequence-469357-        3889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/RE cassette; Const. mNG;  pRAD27-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO263
FEATURES             Location/Qualifiers
     source          1..3889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(2134..2925,2926..2994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2757..2776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2995..3099)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3202..3790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3691..3710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagatgg tagagccaca aacagccggt acaagcaacg
       61 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
      121 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac cgtctcactg
      181 aactggccga taattgcaga cgaacgcctt gtgaaattgc aaatatggtg atttgaaacg
      241 tttcctagtg cagcaggatc acagataacg tgtaaagggc ttagcagttg ataatcctct
      301 ctagttaaga cctaaacaaa atgctgtcac taaccgtagt attaaatgac acactttggt
      361 gactttcgtt aatggggatg tggtagtggc cattgccaat aaacaaaaag aacagggaaa
      421 gaagtagaaa gtgatataag tttgcttgcc acttttcgtt tttcacgaaa aaaacaggcg
      481 aaaaaaaatg ctagacaagt acccggctga atcacacctc gttaacagtg actttcggtg
      541 acagataccc gattgggcac ccggctggta agttatgata gaaagccaac gctgtactat
      601 tggcttagct atggcaatat tttgattatc agctagtttt attaacgtta taattagtgt
      661 aaccagtttt tcatctattt catttatttc atttatttac tttaattgca gatcccccta
      721 acgcgtttaa agcttttatt cactagctta tgtatttttt ataggaaacg cgacgcgtaa
      781 catcgcgcaa atgaaggttt tgatgtatta taatgaggta ttcttcctta tatacatcga
      841 tgaaaagcgt tgacagcata cattggaaag aaataggaaa cggacaccgg aagaaaaaat
      901 agatctatgg tttcaaaagg ggaggaggat aatatggcga gtcttcctgc cacgcatgag
      961 ctgcatatct tcggatctat taatggtgtc gactttgata tggtaggaca aggaaccggg
     1021 aaccccaatg acgggtatga agagctaaat ctaaagagta caaaaggaga tttacagttt
     1081 tctccttgga tacttgttcc ccacattgga tatggatttc atcaatatct tccttatcca
     1141 gacggaatga gcccgttcca ggccgctatg gttgacggct ccgggtatca agtacaccgt
     1201 accatgcagt ttgaggatgg ggcatccctg accgtaaatt acaggtacac ctacgagggc
     1261 tcacacataa agggtgaggc gcaggtaaag ggtactggat ttcctgcgga cggcccagta
     1321 atgacaaatt cacttacagc agcggattgg tgcaggagca aaaagaccta tcccaatgac
     1381 aaaactatca taagtacctt taaatggtcc tatacgacag gtaacgggaa gagatatagg
     1441 tcaacggcaa ggacaacgta tactttcgcg aagccaatgg cagcaaacta tctgaagaat
     1501 caacctatgt acgtgttcag aaagaccgag ttgaaacatt ccaagacaga gttaaatttc
     1561 aaagaatggc aaaaggcttt tactgacgta atggggatgg atgaattgta taagggatcc
     1621 taactcgaga gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata
     1681 gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata
     1741 cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg
     1801 ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaatgctgag
     1861 catgagacgg aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata
     1921 ccgatgactg cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca
     1981 ccactgcata tatggcatat cacaacagtc caactagtgc actgcagtac agcggccgcg
     2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc
     2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3121 acctgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgt
//
