LOCUS       sequence-469364-        3564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL2/RE cassette; ABA systems; 
            phlfO7-pLEU2m-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO236
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1809..2600,2601..2669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2432..2451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2670..2774)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2877..3465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3366..3385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagaata aatcgcagcc aagtgagtga atagatgacg
       61 caccacggtc agacacggcc acatcgtatc tcacaggagc aagcgcgata ggagcactca
      121 cacatagtac ggtgatccgc tgactccttt gcccaaataa gacgtgagcc cgtctcagat
      181 gcacacactg gcttaagatg acaacgggat gatacgaaac gtaccgtatc gttaaggtga
      241 atgatacgaa acgtaccgta tcgttaaggt caatgatacg aaacgtaccg tatcgttaag
      301 gtcaatgata cgaaacgtac cgtatcgtta aggtgtatga tacgaaacgt accgtatcgt
      361 taaggtagat gatacgaaac gtaccgtatc gttaaggtcg atgatacgaa acgtaccgta
      421 tcgttaaggt cggatctcag ctagctttga caatattatt taaggaccta ttgttttttc
      481 caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac atttcagcaa
      541 tatatatata tatatttcaa ggatatacca ttctaagatc tatggtttca aaaggggagg
      601 aggataatat ggcgagtctt cctgccacgc atgagctgca tatcttcgga tctattaatg
      661 gtgtcgactt tgatatggta ggacaaggaa ccgggaaccc caatgacggg tatgaagagc
      721 taaatctaaa gagtacaaaa ggagatttac agttttctcc ttggatactt gttccccaca
      781 ttggatatgg atttcatcaa tatcttcctt atccagacgg aatgagcccg ttccaggccg
      841 ctatggttga cggctccggg tatcaagtac accgtaccat gcagtttgag gatggggcat
      901 ccctgaccgt aaattacagg tacacctacg agggctcaca cataaagggt gaggcgcagg
      961 taaagggtac tggatttcct gcggacggcc cagtaatgac aaattcactt acagcagcgg
     1021 attggtgcag gagcaaaaag acctatccca atgacaaaac tatcataagt acctttaaat
     1081 ggtcctatac gacaggtaac gggaagagat ataggtcaac ggcaaggaca acgtatactt
     1141 tcgcgaagcc aatggcagca aactatctga agaatcaacc tatgtacgtg ttcagaaaga
     1201 ccgagttgaa acattccaag acagagttaa atttcaaaga atggcaaaag gcttttactg
     1261 acgtaatggg gatggatgaa ttgtataagg gatcctaact cgagagtgct tttaactaag
     1321 aattattagt cttttctgct tattttttca tcatagttta gaacacttta tattaacgaa
     1381 tagtttatga atctatttag gtttaaaaat tgatacagtt ttataagtta ctttttcaaa
     1441 gactcgtgct gtctattgca taatgcactg gaaggggaaa aaaaaggtgc acacgcgtgg
     1501 ctttttcttg aatttgcagt ttgaaaaatg ctgagcatga gacggaaatc tgctcgtcag
     1561 tggtgctcac actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa
     1621 ctaagcaaga cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa
     1681 cagtccaact agtgcactgc agtacagcgg ccgcgattat caaaaaggat cttcacctag
     1741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1921 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
     1981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa
     2821 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3001 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3541 tcccctgatt ctgtggataa ccgt
//
