LOCUS       sequence-46939-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Uttamapinant C, White KA, Baruah H, Thompson S, Fernandez-Suarez M, 
            Puthenveetil S, Ting AY
  TITLE     A fluorophore ligase for site-specific protein labeling inside 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.
  PUBMED    20534555
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 15. 107(24):10914-9."
COMMENT     Sequence Label: pYFJ16-LplA(W37V) (for E. Coli expression)
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(184..288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     356..374
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(398..412,413..418,419..435,436..442)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    450..466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             485..496
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             509..529
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
       61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      361 gcgtatcacg aggccctttc gtcttcacct cgagaaatca taaaaaattt atttgctttg
      421 tgagcggata acaattataa tagattcaat tgtgagcgga taacaatttc acacagaatt
      481 cattaaagag gagaaattaa ctatgaaaca tcaccatcac catcaccata tgtccacatt
      541 acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat
      601 ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc gttcgcaatg ccgacacggt
      661 agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga
      721 taacgtccgc ctggcgcggc gca
//
