LOCUS       sequence-469456-       15391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15391)
  AUTHORS   Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, 
            Mushinski JF
  TITLE     Phorbol ester-induced myeloid differentiation is mediated by protein
            kinase C-alpha and -delta and not by protein kinase C-beta II, 
            -epsilon, -zeta, and -eta.
  JOURNAL   J Biol Chem 1993 Sep 25;268(27):20110-5.
  PUBMED    8376369
REFERENCE   2  (bases 1 to 15391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "1993-09-25"; volume: "268(27)"; pages: "20110-5"
COMMENT     Sequence Label: pMTH PKC epsilon
FEATURES             Location/Qualifiers
     source          1..15391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3525..3919
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5538..5557)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     polyA_signal    5758..5892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5782..5801)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5836..5855
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6317..6337)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6467..6532
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(6952..7746)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7063..7082)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7673..7692
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8103..8432)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8417..8437
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(9319..9515)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(9327..9537)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9333..9468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9579..10167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9659..9678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10338..11129,11130..11198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10961..10980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11199..11303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11371..11389
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtccaaaag gaacgtctgt ggccggggtc ccaccgcatg gcgcagcgac caggctgtgg
       61 gcttcaagct caccgcctcg cagattttga tcttaagaag gccattgaac actaccatgg
      121 tcggggcggg ggtcactccg tccgccccga gggcaggatt gaagagcagg agcaggcgcg
      181 ccgggagcct gggggacctc gtgccgaatt aattcagttt ctcagggcat caggtcttca
      241 ccaaagtagg agaagccttt aaattcttcc tggttgatct gcttaatgat tgcttcatcc
      301 acaagtgtaa gtattggctc ttcccgcgta aagtcttggt caaagttatt gacatctctt
      361 ttggttttaa ttctcggctt gaaggggggc ttgattttct tctgctccag cagtacccag
      421 tcaatctcct tgaagaatgg atgttgcttg atggcgtcct ccccgttctg cgctgccaca
      481 cagcccaggc gcttgtgcgg gttcttggtc atgaaagctt tcaggatgct gacagcttcc
      541 ttgctaagcc agacaggata gagaacatca tcatgaagga tggattcgaa caagtcgtcc
      601 tcgttgtcag cttcaaaggg gggctgccca gccatcatct cgtacatcag cacccccagg
      661 gcccaccagt ccactgaggg gccgtactcc aactcctgta ggatctctgg agctatgtag
      721 tcaggagtcc cacagaaggt ggtagttgtc acaccattca taatcccttc cttgcacatc
      781 ccaaagtcag ccagcttgca gtggccttct gcatctagaa ggatgttgtc cagtttcaaa
      841 tccctgtaga tcactccgtg ctggtggaga aacatgaggg ctgatgtgac ctctgcggca
      901 tagaaccgag aacgaggctc atcaaatttt cgggaccgct gaatctggaa catgaggtct
      961 ccaccattta catattccat gacgaagaag aggcggtcct tggtctggaa gcagcaatag
     1021 agttgggtta gataagggtg tttccgagcc agagccaaaa tcctcttctc tgtcattgtg
     1081 cagtccacat catcgtcttg taggataacg tccttcttca agaccttcac agcgtagact
     1141 tcatctttgc ctttgagttc cgccaacatg accttgccaa agctgccttt gcccaacacc
     1201 ttgatgaagt tgaactcatc cagccccaag cgcttggcct ggcctggccg gacttccccg
     1261 ttctctccgg ggcttgccag ctggccatcg gtggccgacg acgctcggtg ctcctctcct
     1321 cggttgtcaa atgacaaggc cttccgaatg ttgttttcaa gttcttttag ttcctggtca
     1381 caaggggagg tgggcgctga cttggatcgg tcgtcttcag atggggagtt tccagaagcc
     1441 ggctgtgggg actcagcacc agcagcgagc tttttcctcc tttggccact gttggtgatt
     1501 ttgtctggag taacaccaag gtcagccagc actttggcaa ttcctctggc gtctacccca
     1561 cagttgggag ccacattggt ctcacatcgc cggtgaacat tcattttgca gactttacac
     1621 tgcaagccct gccgcaagag gccccagagc agggacccac agtggtcaca gaacgtgggg
     1681 accttgtagt tgtggatccc gaacttgtgg ggcatgttga cgctgaaccg ttgggagccc
     1741 acctcgtcag gggtttcctg tttcttcagc ccagcgcact ttgtaataat gagctcatga
     1801 catcgcttgt ggacaacgca agtgcaaact tgacattgat atccctgttt tcctatgaca
     1861 ccccagatga aatctctgca gtgggagcag taggtgggtt gccgcaagta ggtggccatg
     1921 aacttgtggc cattgacctg gtggaccctg cgcctgacag ccccttgccg cttccttggc
     1981 cgcatacgct ccctgaacac tcgttcttca ttgtctttag gggcttcacc cgatgatccc
     2041 gagagatcga tgatcacgta cacttttcct tctggctcca ggtcaatcca gtcctcgaag
     2101 tgacggctcc cattctgcag cagctcctcg aactggatgg tgcagttggc cacgaagtcg
     2161 tcgtagccga taggagcgtc gtgaaagaca gccagctcga tcttgcgccc attgcacaca
     2221 tcggtgacga actcatcgtg ccaggccggg ctgttggtct tttgcttggt ggctgtttgg
     2281 ccgatgcgcg agtcgtccac gttaagggca atgtaggggt ccaaaaggaa cgtctgtggc
     2341 cggggtccca ccgcatggcg cagcgaccag gctgtgggct tcaagctcac cgcctcgcag
     2401 attttgatct taagaaggcc attgaacact accatggtcg gggcgggggt cactccgtcc
     2461 gccccgaggg caggattgaa gagcaggagc aggcgcgccg ggagcctggg ggacctcgtg
     2521 ccgaattgat cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc
     2581 ttcttttcta tggttaagtt catgtcatag gaaggggaga agtaacaggg tacagtttag
     2641 aatgggaaac agacgaatga ttgcatcagt gtggaagtct caggatcgtt ttagtttctt
     2701 ttatttgctg ttcataacaa ttgttttctt ttgtttaatt cttgctttct ttttttttct
     2761 tctccgcaat ttttactatt atacttaatg ccttaacatt gtgtataaca aaaggaaata
     2821 tctctgagat acattaagta acttaaaaaa aaactttaca cagtctgcct agtacattac
     2881 tatttggaat atatgtgtgc ttatttgcat attcataatc tccctacttt attttctttt
     2941 atttttaatt gatacataat cattatacat atttatgggt taaagtgtaa tgttttaata
     3001 tgtgtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt
     3061 cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt
     3121 tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg
     3181 ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat
     3241 tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg
     3301 cttttatttt atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc
     3361 taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg
     3421 tgctggccca tcactttggc aaagaattca ccccaccagt gcaggctgcc tatcagaaag
     3481 tggtggctgg tgtggctaat gccctggccc acaagtatca ctaagctcgc tttcttgctg
     3541 tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt
     3601 atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt cattgcaatg
     3661 atgtatttaa attatttctg aatattttac taaaaaggga atgtgggagg tcagtgcatt
     3721 taaaacataa agaaatgaag agctagttca aaccttggga aaatacacta tatcttaaac
     3781 tccatgaaag aaggtgaggc tgcaaacagc taatgcacat tggcaacagc ccctgatgcc
     3841 tatgccttat tcatccctca gaaaaggatt caagtagagg cttgatttgg aggttaaagt
     3901 tttgctatgc tgtattttac attacttatt gttttagctg tcctcatgaa tgtcttttca
     3961 ctacccattt gcttatcctg catctctcag ccttgactcc actcagttct cttgcttaga
     4021 gataccacct ttcccctgaa gtgttccttc catgttttac ggcgagatgg tttctcctcg
     4081 cctggccact cagccttagt tgtctctgtt gtcttataga ggtctacttg aagaaggaaa
     4141 aacagggggc atggtttgac tgtcctgtga gcccttcttc cctgcctccc ccactcacag
     4201 tgacccggaa tctgcagtgc tagtctcccg gaactatcac tctttcacag tctgctttgg
     4261 aaggactggg cttagtatga aaagttagga ctgagaagaa tttgaaaggg ggctttttgt
     4321 agcttgatat tcactactgt cttattaccc tatcataggc ccaccccaaa tggaagtccc
     4381 attcttcctc aggatgttta agattagcat tcaggaagag atcagaggtc tgctggctcc
     4441 cttatcatgt cccttatggt gcttctggct ctgcagttat tagcatagtg ttaccatcaa
     4501 ccaccttaac ttcatttttc ttattcaata cctaggtagg tagatgctag attctggaaa
     4561 taaaatatga gtctcaagtg gtccttgtcc tctctcccag tcaaattctg aatctagttg
     4621 gcaagattct gaaatcaagg catataatca gtaataagtg atgatagaag ggtatataga
     4681 agaattttat tatatgagag ggtgaaaccc tcaaaatgaa atgaaatcag acccttgtct
     4741 tacaccataa acaaaaataa atttgaatgg gttaaagaat taaactaaga cctaaaacca
     4801 taaaaatttt taaagaaatc aaaagaagaa aattctaata ttcatgttgc agccgttttt
     4861 tgaatttgat atgagaagca aaggcaacaa aaggaaaaat aaagaagtga ggctacatca
     4921 aactaaaaaa tttccacaca aaaaagaaaa caatgaacaa atgaaaggtg aaccatgaaa
     4981 tggcatattt gcaaaccaaa tatttcttaa atattttggt taatatccaa aatatataag
     5041 aaacacagat gattcaataa caaacaaaaa attaaaaata ggaaaataaa aaaattaaaa
     5101 agaagaaaat cctgccattt atggcagaat tgatgaacct ggaggatgta aaactaagaa
     5161 aaataagcct gacacaaaaa gacaaatact acacaacctt gctcatatgt gaaacataaa
     5221 aaagtcactc tcatggaaac agacagtaga ggtatggttt ccaggggttg ggggtgggag
     5281 aatcaggaaa ctattactca aagggtataa aatttcagtt atgtgggatg aataaattct
     5341 agatatctaa tgtacagcat cgtgactgta gttaattgta ctgtaagtat atttaaaatt
     5401 tgcaaagaga gtagattttt ttgttttttt agatggagtt ttgctcttgt tgtccaggct
     5461 ggagtgcaat ggcaagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     5521 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     5581 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     5641 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     5701 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     5761 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5821 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5881 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     5941 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctc
     6001 tagtcaaggc actatacatc aaatattcct tattaacccc tttacaaatt aaaaagctaa
     6061 aggtacacaa tttttgagca tagttattaa tagcagacac tctatgcctg tgtggagtaa
     6121 gaaaaaacag tatgttatga ttataactgt tatgcctact tataaaggtt acagaatatt
     6181 tttccataat tttcttgtat agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc
     6241 aagcaagagt tctattacta aacacagcat gactcaaaaa acttagcaat tctgaaggaa
     6301 agtccttggg gtcttctacc tttctcttct tttttggagg agtagaatgt tgagagtcag
     6361 cagtagcctc atcatcacta gatggcattt cttctgagca aaacaggttt tcctcattaa
     6421 aggcattcca ccactgctcc cattcatcag ttccataggt tggaatctaa aatacacaaa
     6481 caattagaat cagtagttta acacattata cacttaaaaa ttttatattt accttagagc
     6541 tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc acagaagtaa ggttccttca
     6601 caaagatccg gaccaaagcg gccatcgtgc ctccccactc ctgcagttcg ggggcatgga
     6661 tgcgcggata gccgctgctg gtttcctgga tgccgacgga tttgcactgc cggtagaact
     6721 ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact cgcgagggga tcgagcccgg
     6781 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     6841 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     6901 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     6961 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     7021 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     7081 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     7141 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     7201 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     7261 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     7321 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     7381 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     7441 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     7501 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     7561 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     7621 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     7681 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     7741 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca
     7801 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     7861 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     7921 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     7981 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     8041 ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaagc
     8101 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     8161 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     8221 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     8281 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     8341 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     8401 cctggggact ttccacaccc taactgacac acattccaca gggtcgaccg atgcccttga
     8461 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8521 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     8581 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     8641 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     8701 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     8761 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     8821 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     8881 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     8941 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     9001 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     9061 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     9121 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     9181 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtccaggca
     9241 ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta
     9301 tccatctttg caaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     9361 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     9421 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     9481 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     9541 ggagcctgga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    10021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10621 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
    10681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10921 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
    10981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11341 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
    11401 caagaattct ggtttttttg tttgtttgtt tggttttggt ttttggtttt tcgagacagg
    11461 gtttctctat atagccctag ctgtcctgga actcactttg ttgaccaggc tggcctcgaa
    11521 ctcagaaatc cgcctgcctc tgcctcctga gtgctgggat caaaggcgtg cgccaccaac
    11581 acccggcttg tttttttttt ttttttttaa tctttaatca cttccttatt aacttttgaa
    11641 ataggatctc atttgtgtgg ccctggctgg ccttgaattt tgcagagatc atctgcctct
    11701 gacttctaag agctgagatt aaagagctac cctaaggcaa gaatttaact gtatagataa
    11761 agactcagtt ccaaatgaag gttttttttt ggatcttccc acgcagaggg tattgctgtg
    11821 ttgtctcctc caagaggaaa tatccggagg aaggagaaac gccctgggta atattatact
    11881 accaaaccag tgggttgata atagaaagac acttgtatct aaaccaccat gaaggaggag
    11941 gaagaggagg aggggggagg aggagaaaaa ggaagaagag aggagaaaga aggggagaag
    12001 aacggagaag aggaggaaga agaggaggaa gagggagagg gggaagagga gggggaagtg
    12061 tgtgtgtggg ggggggggag tgtcatagcc cagcacctgg tgccttctct gcctacagat
    12121 gaactttgta cagaatcaca gcaagagaac gtgggacaaa tacaggagcg ttagtgccag
    12181 gcagtgctgg ttcacagccg ttgtactact gactggatat atggcctggg caagtcactg
    12241 aacctctgaa tcctctgtcc ttgtgtgtaa atagaatttg catctctcta taggtattaa
    12301 atgagaaatt ggtttaactc ctggttctgg cataatagac atattgctgg tggtggttct
    12361 accacagtta agcaaaaacc tgcccaaagt cgtatagctc agaagtgatg gtatgcatga
    12421 gccaacagtg tagctgttct cttgcccagg acacaaggcc tgcctgacag cagactctaa
    12481 tgttactcaa aatcagaagc attaaaaaaa aaagaaagaa agaaatagct gactttattt
    12541 tttttatcta atgcagtccc cacctaaaat gatctctctg gagggagggt accttggttt
    12601 ttaaaaccag cctggagtag agcagatggg ttaaggtgag tgacccctca gccctggaca
    12661 ttcttagatg agccccctca ggagtagaga ataatgttga gatgagttct gttggctaaa
    12721 ataatcaagg ctagtcttta taaaactgtc tcctcttctc ctagcttcga tccagagaga
    12781 gacctgggcg gagctggtcg ctgctcagga actccaggaa aggagaagct gaggttacca
    12841 cgctgcgaat gggtttacgg agatagctgg ctttccgggg tgagttctcg taaactccag
    12901 agcagcgata ggccgtaata tcggggaaag cactataggg acatgatgtt ccacacgtca
    12961 catgggtcgt cctatccgag ccagtcgtgc caaaggggcg gtcccgctgt gcacactggc
    13021 gctccaggga gctctgcact ccgcccgaaa agtgcgctcg gctctgccaa ggacgcgggg
    13081 cgcgtgacta tgcgtgggct ggagcaaccg cctgctgggt gcaaaccctt tgcgcccgga
    13141 ctcgtccaac gactataaag agggcaggct gtcctctaag cgtcaccacg acttcaacgt
    13201 cctgagtacc ttctcctcac ttactccgta gctccagctt caccagatcc tctagagtcg
    13261 acctgcagcc caagctcgcg gatcaattca gtttctcagg gcatcaggtc ttcaccaaag
    13321 taggagaagc ctttaaattc ttcctggttg atctgcttaa tgattgcttc atccacaagt
    13381 gtaagtattg gctcttcccg cgtaaagtct tggtcaaagt tattgacatc tcttttggtt
    13441 ttaattctcg gcttgaaggg gggcttgatt ttcttctgct ccagcagtac ccagtcaatc
    13501 tccttgaaga atggatgttg cttgatggcg tcctccccgt tctgcgctgc cacacagccc
    13561 aggcgcttgt gcgggttctt ggtcatgaaa gctttcagga tgctgacagc ttccttgcta
    13621 agccagacag gatagagaac atcatcatga aggatggatt cgaacaagtc gtcctcgttg
    13681 tcagcttcaa aggggggctg cccagccatc atctcgtaca tcagcacccc cagggcccac
    13741 cagtccactg aggggccgta ctccaactcc tgtaggatct ctggagctat gtagtcagga
    13801 gtcccacaga aggtggtagt tgtcacacca ttcataatcc cttccttgca catcccaaag
    13861 tcagccagct tgcagtggcc ttctgcatct agaaggatgt tgtccagttt caaatccctg
    13921 tagatcactc cgtgctggtg gagaaacatg agggctgatg tgacctctgc ggcatagaac
    13981 cgagaacgag gctcatcaaa ttttcgggac cgctgaatct ggaacatgag gtctccacca
    14041 tttacatatt ccatgacgaa gaagaggcgg tccttggtct ggaagcagca atagagttgg
    14101 gttagataag ggtgtttccg agccagagcc aaaatcctct tctctgtcat tgtgcagtcc
    14161 acatcatcgt cttgtaggat aacgtccttc ttcaagacct tcacagcgta gacttcatct
    14221 ttgcctttga gttccgccaa catgaccttg ccaaagctgc ctttgcccaa caccttgatg
    14281 aagttgaact catccagccc caagcgcttg gcctggcctg gccggacttc cccgttctct
    14341 ccggggcttg ccagctggcc atcggtggcc gacgacgctc ggtgctcctc tcctcggttg
    14401 tcaaatgaca aggccttccg aatgttgttt tcaagttctt ttagttcctg gtcacaaggg
    14461 gaggtgggcg ctgacttgga tcggtcgtct tcagatgggg agtttccaga agccggctgt
    14521 ggggactcag caccagcagc gagctttttc ctcctttggc cactgttggt gattttgtct
    14581 ggagtaacac caaggtcagc cagcactttg gcaattcctc tggcgtctac cccacagttg
    14641 ggagccacat tggtctcaca tcgccggtga acattcattt tgcagacttt acactgcaag
    14701 ccctgccgca agaggcccca gagcagggac ccacagtggt cacagaacgt ggggaccttg
    14761 tagttgtgga tcccgaactt gtggggcatg ttgacgctga accgttggga gcccacctcg
    14821 tcaggggttt cctgtttctt cagcccagcg cactttgtaa taatgagctc atgacatcgc
    14881 ttgtggacaa cgcaagtgca aacttgacat tgatatccct gttttcctat gacaccccag
    14941 atgaaatctc tgcagtggga gcagtaggtg ggttgccgca agtaggtggc catgaacttg
    15001 tggccattga cctggtggac cctgcgcctg acagcccctt gccgcttcct tggccgcata
    15061 cgctccctga acactcgttc ttcattgtct ttaggggctt cacccgatga tcccgagaga
    15121 tcgatgatca cgtacacttt tccttctggc tccaggtcaa tccagtcctc gaagtgacgg
    15181 ctcccattct gcagcagctc ctcgaactgg atggtgcagt tggccacgaa gtcgtcgtag
    15241 ccgataggag cgtcgtgaaa gacagccagc tcgatcttgc gcccattgca cacatcggtg
    15301 acgaactcat cgtgccaggc cgggctgttg gtcttttgct tggtggctgt ttggccgatg
    15361 cgcgagtcgt ccacgttaag ggcaatgtag g
//
