LOCUS       sequence-469585-       10368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of hFGFR4 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi 
            VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, 
            Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova 
            K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, 
            Krejci P
  TITLE     Specific inhibition of fibroblast growth factor receptor 1 signaling
            by a DNA aptamer.
  JOURNAL   Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 
            10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11.
  PUBMED    39759879
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2024-11-28"; volume: "36(1):102405. doi"; 
            pages: " 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pTR01F/hFGFR4-V5
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1601..1722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1764..1784
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1838..2437)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1921..1941)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2418..2437
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2438..2491)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2561..3223)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      3244..3253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3282..3550
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3563..3774)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    4022..4143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4194..4211)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4221..4262)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(5564..5575)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6819..6838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6820..6838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6883..7086)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6912..6932)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7220..7239)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     repeat_region   7822..7856
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8347..8363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8360..8382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8371..8387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8395..8401,8402..8419,8420..8425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8440..8461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8578..8595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8749..9337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8829..8848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9508..10299,10300..10368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10131..10150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaagccata ccaatgggcc ctaaaaaagg aatccagtca
     1381 attccggggc taaacctggc tgccactgtt tctttaggga cttcgttcct gtgaggacag
     1441 gaaaccctgc aacctggttg accagagcaa gggatataag aacccagttt gggaacactg
     1501 ctgtttaaat attaaacagg gatgtcttgt cccccggaag ggagaccaga tgagcttctg
     1561 atagactaat tgacatcatt tgagtcaatt ccagacatga taagatacat tgatgagttt
     1621 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1681 attgctttat ttgtaaccat tataagctgc aataaacaag tttagttaac gcatgataca
     1741 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     1801 tcaatctttc acaaattttg taatccagag gttgatttca ggcaccgggc ttgcgggtca
     1861 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     1921 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     1981 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     2041 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     2101 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     2161 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     2221 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     2281 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     2341 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     2401 gggtggcgag gcgcaccgtg ggcttgtact cggtcatagg gccgggattc tcctccacgt
     2461 caccgcatgt tagaagactt cctctgccct cgcgagatcc ggtggagccg ggtccggcgg
     2521 tgccgtccac ggcagaattg gacgactgag cgcgggatct ggcgaaggcg atgggggtct
     2581 tgaaggcgtg ctggtactcc acgatgccca gctcggtgtt gctgtgcagc tcctccacgc
     2641 ggcggaaggc gaacatgggg cccccgttct gcaggatgct ggggtggatg gcgctcttga
     2701 agtgcatgtg gctgtccacc acgaagctgt agtagccgcc gtcgcgcagg ctgaaggtgc
     2761 gggcgaagct gcccaccagc acgttatcgc ccatggggtg caggtgctcc acggtggcgt
     2821 tgctgcggat gatcttgtcg gtgaagatca cgctgtcctc ggggaagccg gtgcccacca
     2881 ccttgaagtc gccgatcacg cggccggcct cgtagcggta gctgaagctc acgtgcagca
     2941 cgccgccgtc ctcgtacttc tcgatgcggg tgttggtgta gccgccgttg ttgatggcgt
     3001 gcaggaaggg gttctcgtag ccgctggggt aggtgccgaa gtggtagaag ccgtagccca
     3061 tcacgtggct cagcaggtag gggctgaagg tcagggcgcc tttggtgctc ttcatcttgt
     3121 tggtcatgcg gccctgcttg ggggtgccct ctccgccgcc caccagctcg aactccacgc
     3181 cgttcagggt gccggtgatg cggcactcga tctccatggc gggcaggccg ctctcgtcgc
     3241 tctccatggt ggcgtctagc gtaggcgccg gtcacagctt ggatctgtaa cggcgcagaa
     3301 cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc gtggagagcc
     3361 ggctgagtct aggtaggctc caagggagcg ccggacaaag gcccggtctc gacctgagct
     3421 ttaaacttac ctagacggcg gacgcagttc aggaggcacc acaggcggga ggcggcagaa
     3481 cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg gcgggcgcgt gaaggagaga
     3541 tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     3601 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     3661 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca cccgttcaat
     3721 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     3781 gggcaccgga gcgatcgcag atccttcgcg gccgcggatc cgatttagaa ggtagctaac
     3841 caagttcctc tttcagaggt tatttcaggc catggtgctg cgcagatcag cggaagagcg
     3901 cccaatacgc aaacggatcc ttatcgattt taccacattt gtagaggttt tacttgcttt
     3961 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4021 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4141 ttatcatgtc tgctcgaagc ggccggccgc cccgactcta gggtttaaac tcaatggtga
     4201 tggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
     4261 ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat atctgcagaa
     4321 ttgtcttgac ccttggctgt ctgcacccca gacccgaagg ggaaggagct ggatcccaat
     4381 ggcagggggt cgtggctgaa gacagaatcg ctggaggagc aggtgctgct ggcgtcccca
     4441 ccagaggggg aatagggtcc gaaggtcagg cggaggtcga ggtactcctc agagacggcc
     4501 agcaggacct tgtccagcgc ctccaccagc tgcttgaagg taggcctctg ggagggcgct
     4561 gcgtgccagc actcacgcat cagcccgtac agctctgggg ggcagtgtgg gggtcggtcc
     4621 atccgatgtc cctcccgcag cagcgagaac agctcctcca ccgggatgcc aggatacggg
     4681 gagcccccga gggtgaagat ctcccatagc aggatcccaa aagaccacac gtcactctgg
     4741 tgtgtgtaca cccggtcaaa caaggcctcg ggcgccatcc acttcacagg caggcggccg
     4801 ttgctggttt tcttatagta gtcaatgtgg tggacgccgc gggccagccc aaagtcagca
     4861 atcttcatca cattgtcctc agtcaccagc acattgcggg cagccaggtc ccggtggata
     4921 cacttccggg actccagata ctgcatgcct cgggccacct ggtaggcgca ggagaccagg
     4981 actgggaagg agagcggccc ctcactgctc cgaggaccgt cggggctgag gtcggggcct
     5041 ggggggcgcc gggcccgcag gaactcccgc aggtttccct tggcggcgca ctccacgatc
     5101 acgtacaggg gcccttcctg ggtgcagaca ccaagcaggt tgatgatgtt cttgtgtcgg
     5161 ccgatcagct tcatcacctc catctccgag accaggtcgg ccaggtcctt gtcagaggcg
     5221 ttgtctttga gcatcttgac ggccacagtg ctggcttggt caggccgggc agggtccatg
     5281 ccaaaggcct ctgcacgtac tacctggcca aagcagccct cgcctagggg cttcccaagc
     5341 accagcctgt cccgggggaa ctcccatagt gggtcgagag gtagatctag actcacgagg
     5401 ccggcgagca aggcggggcc gctggaggag agacgcacgc ctcgtaccag ggatgagctt
     5461 gacttgccgg aagagcctga ctccagggag aactgtcggg ccagagggaa gcgggagagc
     5521 ttctgcacag tggcgggcgg gcgggggtgc cggccgtgga gcgcctgccc tcgatacagc
     5581 ccggccagca gcaggagcac agccaaggcc agggagcccg acgcgtacag gatgatgtcc
     5641 gtatacctgg cctcgggcgc tgctgcggtc catgtggggt cctcctctgg cagcaccgtg
     5701 agccaggcag actggtagga gaggccgatg gaattgcctg cgaggcaggt gtactcgcct
     5761 gcgtcctcgg ctgacacgtt ccgcaggtac aggacctcca cctctgagct attgatgtct
     5821 gcagtcttta ggacttgcac ataggggaaa ccgtcggctc cgaagctgct gccgttgatg
     5881 acgatgtgct tcagccactg gatgtggggc tgggcatcgc tgtacacctt gcacagcagc
     5941 tccacgtcgc tgcccaccac ggctgtggtg ttggccggga gcccggcctg caggatgggc
     6001 cggtgcgggg accgctccag cacatctagc aggtagttat agcggatgct gcccacagcg
     6061 ttctctacca ggcaggtgta tgtgccgcgg tccgagggca ccacgctctc catcacgaga
     6121 ctccagtgct gatggcgcag ccgaatgcct ccaatgcggt tctccccatg aaaggcctgt
     6181 ccatccttaa gccagcggat ggtgggcgtg gggttgcctg cagctggaca gcggaacttg
     6241 acggtgttcc ccgcaggtac tgcatgcagt ttcttctcca tgcgctgggg gtgtgtccag
     6301 tagggtgctt gctgggggta actgtgccta ttcgaggggt ccctatggga cttggggtcc
     6361 tcatcatcgt tgctggaggt caaggagtca cctgtaatca aggtgagatt ctgcaggacg
     6421 atcatggagc ctcgtgccag gcagaggtag cggccagcat cctcaggtag gaagctggca
     6481 atctctaggc ggcccctcca gccccgtaca cggccagcag gtgccaggcg actgccctcc
     6541 ttgtaccagt ggccaccacg ctcagcccgc ccacagcaca gccgcacagg ctgtccaagg
     6601 gctactgtca gctcctgctc ttgctgctcc aggctgggag ccaggcaggg ctcaagctcc
     6661 acttcctcag aggcctccag ggacaagact ggaggcccag gcacactcag caggaccccc
     6721 aacagggcca gcagcagccg catctccttc tcacagctct caggtgaagg gtactggatc
     6781 cgagctcggt accaagctta actagccagc ttgggtctcc ctatagtgag tcgtattaat
     6841 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
     6901 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt cacgacattt
     6961 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     7021 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     7081 catcaccatg gtaatagcga tctggaaatt cccggaaagt ccccggaaat tcccggaaag
     7141 tccccggaaa ttcccgctag cgagctcagg taccggccag ttaggccaga gaaatgttct
     7201 ggcacctgca cttgcactgg ggacagccta ttttgctagt ttacgtagat gtactgccaa
     7261 gtaggaaagt cccataaggt catgtactgg gcataatact agtggccgtg gaggcctttt
     7321 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     7381 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     7441 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     7501 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     7561 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     7621 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     7681 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     7741 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     7801 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     7861 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     7921 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     7981 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     8041 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     8101 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     8161 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     8221 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     8281 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     8341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcat
//
