LOCUS       sequence-47-expo       10734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD18_49
FEATURES             Location/Qualifiers
     source          1..10734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3816..3836
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3924..6971
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(3926..3948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8308..8330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8430..8449
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8527..8545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8613..8717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8718..8786,8787..9578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8936..8955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9749..10337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10238..10257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10491..10508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10625..10646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10661..10666,10667..10684,10685..10691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10699..10715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10704..10726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(10723..10734,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10723..10734,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgcaagc ttgggctgca ggtcggctat aataagttct tgaataaaat
       61 aattttcccg acaaaacatg agtatttctt tcgaaaataa aagtgcaggc taattagaga
      121 ttattctgta attaactgca taatttgtca cgtgccatag ttttacattc cactacgtca
      181 tagttcttaa aatactaatc tcctgaaaat agaagtaggt gaagaaagtt taattatcag
      241 ttctaaaatg acaattgatc tttggaatat gttctgaaac taccgatcat tgaacagatg
      301 ctatttgaat gatatagaat tgtatatttg caatttctga aacgcgttct taaaggcaca
      361 cagattaatt caaaagggtc tggccgcaaa aaggtttatg gtggccgatt ttgagttttg
      421 tgtgtgattg ctttttcaca atcagtgttt tcaggattat gtgatgaact agatcttcaa
      481 gtttcgttac atttcatatg ttttcggaac tcacgaagta catattgggt attgtgctca
      541 aaaaattcag caatcagctt cgctccgctg actttagaac ccaaaaaaat agtatggcca
      601 aactgactgt gttacgatca tttcaatttt tcaatacata tttaagattt ctaagagtaa
      661 gaaggtcaaa aactgttctg gaatacatat atatttttca ggttacaatt agtcaaaaag
      721 tgcactgaaa tatacgtttt aatttcacga ataacccaat tagttcaatg tatttttggt
      781 caaccaacgt taaagtttgg cttccaacca attatcattt ctgatcaacc acaatgtttt
      841 ttctttatct gcaagttaat tttttatttt tatccagatg tttggcatat ttttcaattc
      901 ttcactagcg cccacttctt gcacttccgg cgccctgaat ctaatgcatc tgttgcaaga
      961 attgaaagac caatcaacac attgttttct tcacgagata ctgaagaaaa tgaataaaaa
     1021 cagagaaaaa gagccatgtg attagtgaca actgttgcta acagatacca tagcttggac
     1081 ttggtacgtg atggcaacgt atgggtcaac aaaaatgatt gcagaggggg tgcaaaacag
     1141 tcaagtcgag aaaatatgaa aaacagaaaa caaagaacag aaaaatgggt ttgagagtca
     1201 gtataattta taaaagaaaa attgtacata gaaattaacc atttttgtag aagaagttat
     1261 ttttcaagca tcgttaaaaa ttattcaaag caccttattt catatttaat tttaaacatg
     1321 gttaaatgaa caacacggtg cgcaatcagg aaaacttgaa atctgaaact gttgttgtga
     1381 tcttcttcgc aactgttcag atagcactag tgtaatgtta agagtgcgcg aatataatgg
     1441 aatataatgg atcacacctc ctgccatcag gtaaacgtct ctgttatcac atatttccaa
     1501 ctattaaatt tttacctttt acagttttac atttttttga aaaaagtaac tttttgtctt
     1561 caaaatccct gacgaaaata tcaaatattt taatcgagac tgcagaggaa ccgattgatg
     1621 atttggaaaa tccagcttta cctgtgtaag aactgaaaag tttcataacc ctagggtatt
     1681 cccagttaca ttccccactg gctaacaata gcacccagtt tttcatcacc tttcttcaaa
     1741 tttctcggcg atttgttaaa aacaaaattt gtgtcccttc tctgatatct ctatgtctct
     1801 aaacacaagt tcatcggaaa acgaaggagg gtaggtgttg gttgggctcc cgaagtgaaa
     1861 atagaagagc aagaatagaa tattagagag agagtgcaga gagggcggga tagctcccgg
     1921 gattccgttt tcttcttctt tatcttcaac gatgatgtgt gtgcgtgttg tatagattct
     1981 gttgctcccc cacaactcgc tccgaaggct caatacaatt caattgatat tggaggagag
     2041 cctaccggag tgggaggata agaagaaaca taagaagaag aagaagaaga agcatgcttc
     2101 tggtttttga tgctatgaaa acggcacaaa aagatgattg aggtcccttt tcaatacctt
     2161 ctctcatctt tcaaatccca ttgaaaccta aaacttctca ccacgcttta ccattgttct
     2221 ccaaaaactt atagcaatgt ctataacttt tttatctctg aaaagcagtg ttccattttt
     2281 ctttttccta ttttatttca attgtttctc acatttcgtt tggattcttt gcttgtcaac
     2341 cagcttcttc ttccactttt accgtctaat tttcagggca gggagccatc aaacccacga
     2401 ccactagatc catctagaaa tgtctggaaa tccagacgca ttcgaaaatg atccaggatt
     2461 cccattcttg ggaatctcga gagaggccag agctgccact gctgctcgtc cttttgattc
     2521 caagaagaac tgctggattc cggatccaga agatggtaag tcttgatttc gaattgaaaa
     2581 ttttaaagaa aaattttgaa ccacgtgctg taatatttgg caagaatatt ttcccataat
     2641 tttaatagta actgtgatat gttctaattt tagaaatcag ttgtcaacgc taaaaatgta
     2701 agggttacaa tagtggtatt gcaaagagtt atgatggtta agtttgtaaa tacttttttt
     2761 ctgttaatga aactaatttg gatacaaatt tttcatttga tctaaacaga acatcttaat
     2821 caaaaaacac tttaaaactc ttgctttgat attgtttgtt caatttccta attcttacca
     2881 aaccaattca agctaacttt cattatgtcc attccattaa caaaaactat tattacacct
     2941 tctctctcat cgccagtgtc cacattcatc ctactctcct acgctttcat ctgtgtctct
     3001 cagatctgca ttttatctat gatacatcac actttccacc gaccccgccg ggcaaccagt
     3061 ggtggcggat ttttgaacat ctgaggttct ctcatctctc ggtcatctga tgaagcacat
     3121 acacacatac acacaacggc actttgtaat ctttccggag aagcgggcag gcacacacgt
     3181 acgctatgaa gccggagcaa gagcttctcc tgccgtttga actgcggctt ctgctgttgg
     3241 caggaaagat gatgagattt cttggaattt aagattgaga tatttgacgt cccacatttt
     3301 atggatggac aacaatatgt acatgttttg aagatttaat attgaaatgt ggatatgaaa
     3361 tcggaattta aaaagtcaag gtcaataaaa tggtaattct gaccagatct attattcgag
     3421 gatgacaaat aaaactagga ttaaatcaaa aatactaaat ttgaaaatga aaaagaaaag
     3481 attgttctat aacttataaa tctcacaata atgatttttg tacagaaaca aaaactaaat
     3541 gacataatcg aataaaacat tctgatttta atcagccgca tgcgacaaaa catatatttc
     3601 ttggaggcta atattaaatt tcaggattcg tcgccgccga gatccaatcc accaccggcg
     3661 aacaggtgac cgtggtaact gtgaagggaa accagattac cgtcaaaaaa gatcaatgcc
     3721 aagagatgaa cccgccaaaa ttcgacaaga ccgaggatat ggctaacttg acctttttga
     3781 atgagggggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     3841 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg
     3901 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3961 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4021 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg
     4081 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg
     4141 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca
     4201 ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg
     4261 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
     4321 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
     4381 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
     4441 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
     4501 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac
     4561 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg
     4621 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat
     4681 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
     4741 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt
     4801 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca
     4861 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc
     4921 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc
     4981 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
     5041 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc
     5101 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
     5161 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa
     5221 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
     5281 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt
     5341 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc
     5401 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
     5461 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
     5521 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc
     5581 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
     5641 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt
     5701 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa
     5761 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg
     5821 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta
     5881 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg
     5941 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag
     6001 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc
     6061 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga
     6121 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
     6181 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
     6241 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
     6301 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag
     6361 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg
     6421 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta
     6481 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc
     6541 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
     6601 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc
     6661 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
     6721 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
     6781 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
     6841 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
     6901 cgtcagtatc ggcggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt
     6961 gtcaaaaata ataggggccg ctgtcatcag atcgccatct cgcgcccgtg cctctgactt
     7021 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     7081 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     7141 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     7201 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     7261 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     7321 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
     7381 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
     7441 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
     7501 tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc
     7561 acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag
     7621 tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct
     7681 taaatgacag aatacattcc caatatacca aacataactg tttaaaatta aacatttttc
     7741 taaattttat atgatttctt ttaaatttgc aaaaattact taaatttgaa ttcccgcgca
     7801 aatgagtgac ttcattttct gcattattgt gttttccggc tatattaata ggtatttgtt
     7861 tgtgtttttc tttattttat gattcgaact ccaatttgta aattttcgaa catatttccc
     7921 taaagaaaaa atatgattaa tctggaaaaa ttggaaaatt atttttcaaa taaaaaacaa
     7981 agaaaaaaat gaagaaaaac ctattagttt ggccataaaa cgcaaaaatg tcgaaaatga
     8041 cgtcactcat ctgcgcggga aatcaagaat aattcggcct tttttatttt tttggaaaat
     8101 cgtaaaacat ttagaaaaat tttttaatag ttatagtggg actgtattct gtcatttagg
     8161 gcaaaagcca gagacgctac tccaccgtta acatgaatta tgaatattat tgcgacaaga
     8221 cccaaacatt gataaaccgc aaatctagcc tactagtcgg ccgtacgggc cctttcgtct
     8281 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8341 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8401 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8461 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     8521 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8581 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     9901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10141 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10501 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10621 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agct
//
