LOCUS       sequence-470058-        8210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Library-scale IPTG-inducible single transcript dual-gRNA expression 
            for Type II dCas9 in Synechococcus sp. PCC 7002, genomically 
            integrated next to glpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Hren A, Lollini N, Carper DL, Abraham PE, Cameron JC, Fox JM, Eckert
            CA
  TITLE     High-density CRISPRi screens reveal diverse routes to improved 
            acclimation in cyanobacteria.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2412625122. doi: 
            10.1073/pnas.2412625122. Epub 2025 Mar 21.
  PUBMED    40117303
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-03-25"; volume: "122(12):e2412625122. 
            doi"; pages: " 10.1073/pnas.2412625122. Epub 2025 Mar 21"
COMMENT     Sequence Label: pAPH021
FEATURES             Location/Qualifiers
     source          1..8210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(156..172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(156..172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(204..210,211..228,229..234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(387..404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(558..1146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(638..657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1317..2108,2109..2177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1940..1959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2178..2282)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2350..2368
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2406..2428)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2528..2547
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2741..2763
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2755..2772
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2756..2772
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3211..3222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4122..5204)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDFSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5166..5185
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5234..5268)
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     protein_bind    5295..5314
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5399..5415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        5467..5542
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        5637..5712
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5733..5762
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5778..5795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5851..5868)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(6164..6183)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6175..6990
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6175..6990
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6248..6267)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actggggaca atcaatggtc aaacccaagt gattgagggc attactgatc cggctgatcc
       61 gttacaaatt aacgttgctt ttccgggcgg gtttgaacaa tacaccgccc ttctctagag
      121 tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcgtcc aacgcgcggg gagaggcggt
      361 ttgcgtattg ggcggtcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccagtaa ccgtaaaaag
      541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      721 tttctccctt cgggaatcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2341 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2401 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2461 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2581 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2641 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2701 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2761 acgacggcca gtgaattcga gctcggtacc cgggggacgg ctcccctctg tcaaaccctc
     2821 agtgcggcag gcaaagcggc agaaatttac gataaaacgg gcttggtgtt ggatgcttat
     2881 ttttcggcga cgaaactgaa ttggttatta agctgggcta aggaaaatag tgccattaac
     2941 cccaataatg tcctcgctgg aaccattgac acctgggcct tgtggaacct cacgggaggc
     3001 aaagtccacg ccacagatca cagtaatgcc agccggacaa tgttgctcaa tctcaaccaa
     3061 aaagattggg atccggattt gttggattta ttcgatattc cccgccagat gatgccaacg
     3121 gtgcagtcga gcctagggga atttggcaaa accgatccaa gtttattggg ggcggcaatt
     3181 ccgatcaccg ccatctttgg ggatcaacag gcggccctct atgcccatgg ttgcgatcgc
     3241 cccggcttat taaaatgcac ctatggcaca ggggcgtttt tggtggcgca cacgggcgac
     3301 gagattaaac gctccaaaca taagctccta acgaccatcg cctgcaccca agcccatcac
     3361 gatggcacaa atctcgatat tggctatgcc ctcgaaggga gtatgttcac cgctggggct
     3421 tgtatccaat ggttgcgcga tggcctacaa attatcgaaa cggcggcgga aacgaatgat
     3481 cttgcccaag gggtgaatga taatggcggc gcttattttg ttccggcttt aagtggtctg
     3541 ggagcgcccc actgggatat gagtgccagg ggcgcatttc tgggtttaac gagaggcgtc
     3601 aaaaaagaac acatggtacg ggctgtccta gaggcgatcg cctaccaagc caaagaagta
     3661 gtcgaagcga ttaaccaaga ttccggtacg ccgatccaag aattaaaagt cgatggcggc
     3721 gcgtgcaata acgactttct gatgcaattc caagctgacg tgttgggtat tcccgtggaa
     3781 cgtcccgctg tcctcgatgc cacggcccag ggggcagcct ttgccgctgg attagctgtc
     3841 ggtttttggg atgattacca aaccctggtg caaaaccgca aaattgatta cgtctttaag
     3901 cccagtgcca acgcctccca agcccaagcc catttcaaag tctggcaaaa agccgttgaa
     3961 cgagcgaaaa actgggccta accccctctt gcctacagca tctcccccag gggagaattc
     4021 ttcctgtttc aactccccaa gcttggttga aggctctcaa gggcatcggt cgagatcccg
     4081 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     4141 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4201 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     4261 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     4321 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     4381 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     4441 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     4501 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactaaagtc gccttcccgt
     4561 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     4621 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     4681 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     4741 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     4801 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     4861 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     4921 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4981 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     5041 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     5101 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     5161 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccatgaat
     5221 tgaccctcct ccggctagca ttgtacctag gactgagcta gctgtaaaga ctcccctctg
     5281 gacatctcca aacgaattgt gagcgctcac aattcggaat tcttaacaaa aaagcaggaa
     5341 taaaattaac aagatgtaac agacataagt cccatcaccg ttgtataaat gtgtggaatt
     5401 gtgagcggat aacaatttca cacaatatgc aggtgcgtga tggcacttag aactgcacct
     5461 gctatagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     5521 aaaagtggca ccgagtcggt gctttttttg aacctgtctt attgagcttt ccggcgagag
     5581 ttcaatggga caggttcata tgcaggtgat tcatattata ccctatccca cctgcagttt
     5641 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     5701 ccgagtcggt gctttttttg aagcttgggc ccgaacaaaa actcatctca gaagaggatc
     5761 tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc tccagcttgg
     5821 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     5881 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     5941 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     6001 agtagggaac tgccaggcat caaataaaac gaaagactgg gcctttcgtt ttatctgttg
     6061 tttgtcggtg aactacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6121 tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     6181 catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat
     6241 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
     6301 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     6361 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     6421 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     6481 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     6541 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     6601 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     6661 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     6721 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     6781 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     6841 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     6901 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     6961 tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga
     7021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aatctaacgt
     7081 aaacccattg aatttaaaaa agactttatg actgctttac tgctccatga ccaacattat
     7141 tcccttgatc atgaagcctt tctctcaacc ctcagcaaca cagaaaattt actcattatt
     7201 caagatctag atggcgtttg catggggtta gtcaaagacc ccttaacccg caaaattgat
     7261 cctgactata tccgcgccac acgcaagttt agagaccact tctttgtcct caccaacggt
     7321 gaacatgaag gcagaagggg agtaaatcgc atcgttgaac gggcatttcg caatgttgaa
     7381 gccaaagagg aaacaagcta tttacctggt ttagcagcag ggggtgtgca atggcagaca
     7441 gataatggcc aaatttccca tcccggtgtt agccaagcag aactcgattt ccttgccaca
     7501 gtgccagatt taattggtca aagtttagga caatttttta ctaaatatgt tgatattttt
     7561 cccgctgagc ttcaacctga gctgatccat gcttctgttt tagataatct cgtttcaccg
     7621 acggcaaatt taaacgtcct ggccgaatat ttaggcgatc gccttgagat ttaccaagac
     7681 ctccagcgca ccatggaaac cctgatgaat gatttgctag aaaaagctgg ccaacagggt
     7741 ttagacaata gttttttcgt gcactatgcg cccaatttag gcagagataa tgtggggaaa
     7801 gaaattgtcc gctttgccac agccaaggat tctggcacca cagattttca gtttatggtg
     7861 tgtggtgccg tcaaagaagc gggggtttta gtgctgctga attattacta tgcccaacgc
     7921 acgggccact atcccctagg agaaaccttt aacgcccgcc aagcgcccca aaaccacgag
     7981 gaactattgc aactggtgca agacaatttc gatccgcaat tgatgccttt aatcgttggc
     8041 gtcggcgata cggtcacaag tcacaccgaa ggcgatcaag ttcgacgggg ggggagcgat
     8101 cgcctattcc tgcagctcgt ccaagacatt ggtaaatggg caaagagcgg caatctcgtc
     8161 gtgtatatcg acagctccca gggagaatta aaaaatcgga ttcccttaaa
//
